Comparison of Dot Blot, ELISA, and RT-PCR Assays for Detection of Two Cucumber Mosaic Virus Isolates Infecting Banana in Hawaii

Hu, John; Li, H. P.; Barry, Kerrie; Wang, M.; Jordan, Russell T.

doi:10.1094/pd-79-0902

Cited by 62 publications

(49 citation statements)

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“…CMV, which has a spherical particle of 28-30 nm in diameter containing a ssRNA, is naturally transmitted by aphid vectors or by seed. Hu et al (1995) have identified banana viral isolates as belonging to the subgroup I, DTL serotype. Symptoms depend on the strain of the virus pathogen and temperature.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.)

Helliot

Panis

Poumay³

et al. 2002

Plant Cell Rep

135

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The utilisation of cryopreservation for the eradication of cucumber mosaic virus (CMV) or banana streak virus (BSV) from Musa spp. was investigated. Banana plants, cv. Williams (AAA, Cavendish subgroup), were mechanically infected with CMV or naturally infected with BSV, and proliferating meristems were produced from the infected plants. Excised meristematic clumps were cryopreserved through vitrification using PVS-2 solution. The health status of regenerated in vitro plants was first checked by means of ELISA. The putative virus-free material was subsequently tested a second time following greenhouse acclimatisation. The frequency of virus eradication for CMV and BSV was 30% and 90%, respectively, following cryopreservation. In comparison, the frequency of virus-free plants regenerated directly from highly proliferating meristems, corresponding to a spontaneous eradication rate, reached 0% and 52% for CMV and BSV, respectively. The conventional meristem culture resulted in 0% CMV-free plants and 76% BSV-free plants, while the cryoprotective treatment resulted in 2% CMV-free plants and 87% BSV-free plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the structure of the meristem tips by light microscopy. The cryopreservation method used only allowed survival of small areas of cells located in the meristematic dome and at the base of the primordia.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.)

Helliot

Panis

Poumay³

et al. 2002

Plant Cell Rep

135

View full text Add to dashboard Cite

show abstract

“…The distribution of CMV in all diseased plants tested by RT-PCR was uneven and erratic, again in agreement with serological data from Tonga . Similarly, Hu et al (1995) demonstrated that CMV distribution within banana plants also varies greatly.…”

Section: Discussionmentioning

confidence: 93%

“…ELISA has been shown to be a relatively crude CMV detection method compared with PCR (Hu et al 1995) and it may be that the virus was present throughout the plants studied in Tonga in the early 1990s, but only at a titre high enough for ELISA detection in certain plant parts. The first part of the study reported here is an intensive re-examination, using RT-PCR, of the CMV distribution in a small number of large multistemmed kava plants infected with CMV at two locations.…”

Section: Introductionmentioning

confidence: 99%

Cucumber mosaic virusinfection of kava (Piper methysticum) and implications for cultural control of kava dieback disease

Davis

Lomavatu-Fong

McMichael³

et al. 2005

Austral. Plant Pathol.

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Abstract. Cucumber mosaic virus (CMV) was found by reverse transcription polymerase chain reaction (RT-PCR) to be not fully systemic in naturally infected kava (Piper methysticum) plants in Fiji. Twenty-six of 48 samples (54%) from various tissues of three recently infected plants were CMV-positive compared with 7/51 samples (14%) from three long-term infections (plants affected by dieback for more than 1 year). The virus was also found to have a limited ability to move into newly formed stems. CMV was detected in only 2/23 samples taken from re-growth stems arising from known CMV infected/dieback affected plants. Mechanical inoculation experiments conducted in Fiji indicate that the known kava intercrop plants banana (Musa spp.), pineapple (Ananas comosus), peanut (Arachis hypogaea) and the common weed Mikania micrantha are potential hosts for a dieback-causing strain of CMV. It was not possible to transmit the virus mechanically to the common kava intercrop plants taro (Colocasia esculenta), Xanthosoma sp., sweet potato (Ipomoea batatas), yam (Dioscorea alata), papaya (Carica papaya) or the weed Momordica charantia. Implications of the results of this research on a possible integrated disease management strategy are discussed.

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“…Indeed, the RT-PCR method has proven to be more sensitive than ELISA and dot-blot hybridization (Hu et al, 1995). Also cucumber mosaic virus (CMV) can be effectively detected with different sets of degenerate primers (Choi, et al 1999;Hu et al, 1995;Singh et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Alstroemeria-Infecting Cucumber Mosaic Virus Isolates Contain Additional Sequences in the Rna 3 Segment

Chen¹,

Prins²,

Derks³

et al. 2002

Acta Hortic.

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The coat protein (CP) genes and flanking regions of three alstroemeriainfecting cucumber mosaic virus isolates (CMV-ALS), denoted ALS-LBO, ALS-IPO, and ALS-NAK, were cloned and their nucleotide sequence determined and compared at both nucleic acid and deduced protein level with the published sequences of CMV RNA 3. The sequences of these isolates showed more than 95% nucleotide and peptide sequence homology to each other and to other members of subgroup II CMV. Strikingly, an additional sequence of 218 nt was found in the central region of the 3'-non-translated region (NTR) of RNA 3 of two out of three isolates. The additional sequence appeared to have arisen from RNAs 1 or 2. A subgroup-specific DIG-labeled probe has been developed from this additional sequence and applied to detect subgroup II CMV strains in dot blot hybridization and to differentiate the Alstroemeria isolates containing additional sequences in Northern blot hybridization.

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Comparison of Dot Blot, ELISA, and RT-PCR Assays for Detection of Two Cucumber Mosaic Virus Isolates Infecting Banana in Hawaii

Cited by 62 publications

References 0 publications

Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.)

Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.)

Cucumber mosaic virusinfection of kava (Piper methysticum) and implications for cultural control of kava dieback disease

Alstroemeria-Infecting Cucumber Mosaic Virus Isolates Contain Additional Sequences in the Rna 3 Segment

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