Comparison of DNA probe, monoclonal antibody enzyme immunoassay, and cell culture for the detection of Chlamydia trachomatis

LeBar, William; Herschman, Barry R.; Jemal, Claudia; Pierzchala, J

doi:10.1128/jcm.27.5.826-828.1989

Cited by 51 publications

(14 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, diagnosis of chlamydial infections is based primarily on isolation of the organisms in either HeLa 229 or McCoy cells. The specificities of cell culture methods are high; however, the sensitivities of such assays are typically 90 to 95% compared with those of newer methods of detection (6,10,14,18). The sensitivity of culture depends on factors such as the status of patient symptomology, specimen collection techniques, the number of sites from which samples are obtained, the specific sites from which samples are obtained, specimen transport and storage, and cell culture methods.…”

mentioning

confidence: 99%

Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection

An¹,

Radcliffe²,

Vassallo³

et al. 1992

J Clin Microbiol

View full text Add to dashboard Cite

Clinical samples in transport media from 40 patients exhibiting pathologies potentially caused by Chlamydia trachomatis infection were analyzed for chlamydial nucleic acid, and the results were compared with those of culture. Chlamydial culture was performed by a shell vial centrifugation method with HeLa 229 host cells. Polymerase chain reaction (PCR) assays were used to detect either regions on a 7.5-kb plasmid characteristic of C. trachomatis (plasmid-PCR) or a segment of the 16S rRNA genes (rRNA-PCR). All PCR results were confirmed by hybridization with probes for the specific amplified products in either a Southern or a dot blot format. An RNase protection (RNP) assay was used to detect genus-specific chlamydial 16S rRNA directly from the clinical samples. The PCR assays detected C. trachomatis but not other bacteria, including Chlamydia spp. C. trachomatis was isolated from six samples which were positive by the rDNA-PCR and plasmid-PCR assays. Five of the culture-positive specimens were positive by the RNP assay. Twenty-two samples were negative by all criteria. Surprisingly, nine samples were positive by rRNA-PCR and RNP assays only. Nucleic acid sequencing of the rRNA-PCR-amplified products indicated a close relationship between the variants and C. trachomatis. The data may indicate an unrecognized process in C. trachomatis infection or that these patients were infected by a variant strain of C. trachomatis which lacks the C. trachomatis-specific plasmid.

show abstract

mentioning

confidence: 99%

Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection

An¹,

Radcliffe²,

Vassallo³

et al. 1992

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…1 However, the sensitivity of a single endocervical specimen may be only 70-90. [2][3] Unfortunately, cell culture is labor intensive and requires 48 hours for completion. These problems fostered the development of simplified rapid diagnostic tests which bypassed the issue of organism viability.…”

mentioning

confidence: 99%

The Potential Misuse of DNA Probe for the Detection of Neisseria gonorrhoeae and Chlamydía trachomatis When Used for Test of Cure

Monif¹

1997

Infectious Diseases in Obstetrics and Gynecology

View full text Add to dashboard Cite

show abstract

“…These nonculture methods offer several advantages over conventional culture procedures in that the diagnosis is not dependent on the presence of viable microorganisms for microbial isolation and that turnaround times can be significantly reduced. However, none of these methods is completely reliable, and discrepancies with culture may occur (6,7). Even culture does not detect all active infections (13), but remains the "gold standard" with which nonculture methods are compared.…”

mentioning

confidence: 99%

Value of a DNA probe assay (Gen-Probe) compared with that of culture for diagnosis of gonococcal infection

et al. 1993

View full text Add to dashboard Cite

The Gen-Probe PACE 2 system for Neisseria gonorrhoeae (GP), which uses a chemiluminescently labeled DNA probe, was compared with conventional culture as the method of reference. A total of 1,750 specimens were collected from 496 females and 623 males visiting the outpatient clinic of the Sexually Transmitted Diseases Department of the Westeinde Hospital, The Hague, The Netherlands, during the year 1991. The prevalences of gonorrhea culture-positive men and women were 14.9 and 7.7%o, respectively. The overall positive rate was 8.7%. Sensitivity, specificity, and positive and negative predictive values of GP were 97.1, 99.1, 90.6, and 99.8%, respectively. A total of 12 of 13 patients with positive GP results and negative cultures may have had a gonococcal infection, a conclusion based on clinical symptoms, positive methylene blue smears, and high relative light unit ratios. The DNA probe test can be useful as a suitable screening and diagnostic test for gonorrheal infection in men and women. An advantage of using this DNA probe technique is that simultaneous testing for Chlamydia trachomatis of the same specimen is possible. We also examined whether (all) rRNA had disappeared after adequate treatment for gonococcal and/or chlamydial infection in 30 patients. None of those positive patients showed a positive result in the DNA probe assay after treatment.

show abstract

Comparison of DNA probe, monoclonal antibody enzyme immunoassay, and cell culture for the detection of Chlamydia trachomatis

Cited by 51 publications

References 10 publications

Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection

Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection

The Potential Misuse of DNA Probe for the Detection of Neisseria gonorrhoeae and Chlamydía trachomatis When Used for Test of Cure

Value of a DNA probe assay (Gen-Probe) compared with that of culture for diagnosis of gonococcal infection

Contact Info

Product

Resources

About