Comparison of DNA extraction and amplification from ancient human bone and mummified soft tissue

Lassen, Cadja; Hummel, Susanne; Herrmann, Bernd

doi:10.1007/bf01225603

Cited by 41 publications

(13 citation statements)

References 10 publications

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“…Light, moisture and temperature fluctuations are known to be particularly detrimental to DNA. Studies on ancient human DNA have shown that bones and teeth are the specimens most likely to yield amplifiable DNA and mummified tissue has been shown to be an extremely poor source (Lassen et al, 1994). However, MTB DNA was isolated consistently from the mummified tissues examined here.…”

Section: Discussionmentioning

confidence: 75%

Molecular analysis of Mycobacterium tuberculosis DNA from a family of 18th century Hungarians

et al. 2003

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The naturally mummified remains of a mother and two daughters found in an 18th century Hungarian crypt were analysed, using multiple molecular genetic techniques to examine the epidemiology and evolution of tuberculosis. DNA was amplified from a number of targets on the Mycobacterium tuberculosis genome, including DNA from IS6110, gyrA, katG codon 463, oxyR, dnaA-dnaN, mtp40, plcD and the direct repeat (DR) region. The strains present in the mummified remains were identified as M. tuberculosis and not Mycobacterium bovis, from katG and gyrA genotyping, PCR from the oxyR and mtp40 loci, and spoligotyping. Spoligotyping divided the samples into two strain types, and screening for a deletion in the MT1801-plcD region initially divided the strains into three types. Further investigation showed, however, that an apparent deletion was due to poor DNA preservation. By comparing the effect of PCR target size on the yield of amplicon, a clear difference was shown between 18th century and modern M. tuberculosis DNA. A two-centre system was used to confirm the findings of this study, which clearly demonstrate the value of using molecular genetic techniques to study historical cases of tuberculosis and the care required in drawing conclusions. The genotyping and spoligotyping results are consistent with the most recent theory of the evolution and spread of the modern tuberculosis epidemic.

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Section: Discussionmentioning

confidence: 75%

Molecular analysis of Mycobacterium tuberculosis DNA from a family of 18th century Hungarians

et al. 2003

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“…Species determination was successfully performed with DNA extracts from bones, whereas muscle fibres did not yield similar outcomes. This has already been proven by Lassen et al (1994). They compared DNA extracts from ancient bones and mummified soft tissues by PCR analysis and came to the result that skeletal material should be given the preference for DNA investigations.…”

Section: Resultsmentioning

confidence: 95%

Species identification of processed animal proteins (PAPs) in animal feed containing feed materials from animal origin

Axmann

Adler

Brandstettner

et al. 2015

Food Additives & Contaminants: Part A

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Since June 2013 the total feed ban of processed animal proteins (PAPs) was partially lifted. Now it is possible to mix fish feed with PAPs from non-ruminants (pig and poultry). To guarantee that fish feed, which contains non-ruminant PAPs, is free of ruminant PAPs, it has to be analysed with a ruminant PCR assay to comply with the total ban of feeding PAPs from ruminants. However, PCR analysis cannot distinguish between ruminant DNA, which originates from proteins such as muscle and bones, and ruminant DNA, which comes from feed materials of animal origin such as milk products or fat. Thus, there is the risk of obtaining positive ruminant PCR signals based on these materials. The paper describes the development of the combination of two analysis methods, micro-dissection and PCR, to eliminate the problem of 'false-positive' PCR signals. With micro-dissection, single particles can be isolated and subsequently analysed with PCR.

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“…The results of molecular analysis provided evidence for an equivalent balance of both sexes (Lassen et al, 1997(Lassen et al, , 2000, and even revealed a disproportion of male individuals that died during the last trimester of pregnancy, similar to observations found in modern populations (e.g., Bartels et al, 1990). For the molecular sex determination performed in those studies, the amelogenin gene (Nakahori et al, 1991;Mannucci et al, 1994;Bailey et al, 1992;Lassen et al, 1995;Stone et al, 1996) was amplified, exhibiting a length dimorphism for the Xand Y-chromosome (106 and 112 bp). Here, the advantage over earlier attempts to determine sex from archaeological samples focusing on Y-specific markers (e.g., Hummel and Herrmann, 1991;Honda et al, 2001) was in the simultaneous detection of both an X-and Y-chromosomal sequence.…”

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confidence: 73%

Brief communication: Multiplex X/Y‐PCR improves sex identification in aDNA analysis

Hummel¹,

Herrmann²

2003

American J Phys Anthropol

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This study introduces a polymerase chain reaction (PCR)-based multiplex approach to improve the certainty of molecular sex identification on archaeological skeletal material. We coamplified amelogenin, two X-chromosomal short tandem repeats (STRs) (DXS6789 and DXS9898), and two Y-specific STRs (DYS391 and DYS392). The amplification results of this multiplex approach back each other up, and enable a reliable sex identification. This coamplification of X- and Y-specific markers in a multiplex assay combines the added advantage of positive identification of both female and male individuals with raising the validity of the diagnosis by obtaining multiple data simultaneously. This multiplex system was successfully applied to 3,000-year-old bone material.

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Comparison of DNA extraction and amplification from ancient human bone and mummified soft tissue

Cited by 41 publications

References 10 publications

Molecular analysis of Mycobacterium tuberculosis DNA from a family of 18th century Hungarians

Molecular analysis of Mycobacterium tuberculosis DNA from a family of 18th century Hungarians

Species identification of processed animal proteins (PAPs) in animal feed containing feed materials from animal origin

Brief communication: Multiplex X/Y‐PCR improves sex identification in aDNA analysis

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