Comparison of DNA damage and proliferative capacities in smear samples of HPV positive and negative patients by micronucleus counting and AgNOR staining

Sancer, Okan; Şirin, Mümtaz Cem; Arıdoğan, Buket Cicioğlu; Çetin, Emel Sesli; Yiğit, Ahmet; Tepebaşı, Muhammet Yusuf; Koşar, Pınar Aslan

doi:10.1080/10520295.2020.1800091

Cited by 5 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that the number and size of NOR‐related proteins reflect RNA activity. It has been determined that NOR numbers reflect cell activity increase in malignant events 15,23 . The NORs of metaphase chromosomes are associated with a group of acidic proteins called the argyrophilic NOR region (AgNOR) proteins.…”

Section: Discussionmentioning

confidence: 99%

“…The NORs of metaphase chromosomes are associated with a group of acidic proteins called the argyrophilic NOR region (AgNOR) proteins. When viewed with a light microscope, silver‐stained AgNOR areas appear as black dots that can be measured to quantify rRNA transcription activity 23 . The size and number of AgNOR correlate with the proliferation capacity of cells 24,25 .…”

Section: Discussionmentioning

confidence: 99%

“…When viewed with a light microscope, silver-stained AgNOR areas appear as black dots that can be measured to quantify rRNA transcription activity. 23 The size and number of AgNOR correlate with the proliferation capacity of cells. 24,25 In our study, we used the AgNOR staining method to determine the amount of tissue damage in liver and kidney tissues in the LPS-induced sepsis model.…”

Section: Kidneymentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the protective role of resveratrol against sepsis caused by LPS via TLR4/NF‐κB/TNF‐α signaling pathways: Experimental study

Okan

Doğanyiğit

Yılmaz

et al. 2023

Cell Biochemistry & Function

View full text Add to dashboard Cite

The development and progression of sepsis are multifactorial and influence the immunological, endocrine, and cardiovascular systems of the body. Our knowledge of the key mechanisms involved in the pathogenesis of sepsis has expanded exponentially, yet this still needs to be translated into effective targeted therapeutic regimes. In the present study, we aimed to determine whether resveratrol has positive effects in the experimental sepsis rat model. Twenty‐eight male Spraque–Dawley rats were randomly divided into four groups (n = 7) as follows: control, lipopolysaccharide (LPS) (30 mg/kg dose), resveratrol, and LPS and resveratrol. After the experiment, liver and kidney tissues were collected for histopathological evaluation, blood serums were collected to measure malondialdehyde levels with enyzme‐linked immunosorbent assay, and Toll‐like receptor‐4 (TLR4), tumor necrosis factor‐α (TNF‐α), nuclear factor‐κB (NF‐κB) immunoreactivity density was evaluated immunohistochemically. In addition, messenger RNA expression levels for TLR4, TNF‐α, NF‐κB, interleukin‐1β, and interleukin 6 were measured. In addition, the damage observed in liver and kidney tissue was determined by AgNOR (argyrophilic nucleolar organizer regions) staining. LPS application caused severe tissue damage, oxidative stress, and increased the expressions of proinflammatory proteins and genes we evaluated, while resveratrol application eliminated these negativities. Resveratrol has been proven to suppress the TLR4/NF‐κB/TNF‐α pathway, a possible therapeutic signaling pathway that is important in initiating the inflammatory response in an animal model of sepsis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Kidneymentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the protective role of resveratrol against sepsis caused by LPS via TLR4/NF‐κB/TNF‐α signaling pathways: Experimental study

Okan

Doğanyiğit

Yılmaz

et al. 2023

Cell Biochemistry & Function

View full text Add to dashboard Cite

show abstract

“…As can be seen in these papers, the MN rates are much higher than that presented by the authors of this study. As striking examples, the rates of cervical cells with MN in the same population (Turkish women) is 8.2% (82‰; n = 11) 5 and 1.19‰ (n = 11) 6 in recent publications. The first result is also unusual, that is, very high.…”

mentioning

confidence: 94%