Comparison of disinfective power according to application order of 70% isopropyl alcohol and 10% povidone-iodine

Abstract

BackgroundMany disinfectants have been used clinically in both single and combination applications, but there have been few studies on disinfective power according to sterilization sequence when using a combination of disinfectants. The purpose of this study was to evaluate the disinfective power of a combination of 70% isopropyl alcohol and 10% povidone-iodine (PVP-I) according to sterilization sequence.MethodsTwo hundred healthy volunteers were recruited. Subjects were disinfected with a combination of 70% isopropyl alcohol and 10% PVP-I on both forearms, in varying sequence. The AP group included disinfections on the left forearm with isopropyl alcohol first followed by 10% PVP-I, while the PA group included disinfections on the right forearm with same disinfectants in reverse order. Skin cultures were obtained using cotton swabs 3 min after application of each disinfectant, and then were inoculated on blood agar plates for bacterial culture. Cultures were incubated at 37℃ under aerobic conditions for 48 hours.ResultsThere was no significant difference in the number of positive cultures after the 1st disinfection (AP, 45; PA, 36, P = 0.262) or the 2nd disinfection (AP, 6; PA, 13, P = 0.157), suggesting that there is no relationship between disinfective power and the sequence of the disinfectants used. The number of positive cultures significantly decreased after the 2nd disinfection (P < 0.01), however.ConclusionsThere was no significant difference in disinfective power according to sterilization sequence with 70% isopropyl alcohol and 10% PVP-I in healthy volunteers. The combination of 70% isopropyl alcohol and 10% PVP-I was more effective than disinfection with a single agent regardless of sterilization sequence.

Antimicrobial performance of two preoperative skin preparation solutions containing iodine and isopropyl alcohol

“…IPA provides antimicrobial action through coagulation and/or denaturing of soluble proteins, which has been associated with disruption of cytoplasmic integrity, cell lysis, and interference with cellular metabolism [11]. Based on their respective mechanisms of action, the combination of PVP-I and IPA has been shown to be more efficacious than PVP-I alone [12,13].…”

Kombi̇ne Sali̇si̇li̇k Asi̇t Ve Povi̇don-İyot İçeren Nanoemüljelleri̇n Hazirlanmasi Ve Karakteri̇zasyonu: Ön Çalişma

“…The images of the nanoemulgels are given in Figure 2 below. When the PI studies in the literature are examined, it is seen that PI is generally used at the rate of 0.5% to 10% [12,32,33]. Therefore, in our study, the dose of PI was chosen as 2% in nanoemulgels.…”

Povidon-iodine (betadine) in modern obstetric-gynecological practice

“…Хотя добавление спиртов существенно не увеличивает антисептических свойств повидон-йода [9,13], результаты исследования на 200 здоровых добровольцах показали, что использование 70%-ного раствора изопропилового спирта до или после обработки кожной поверхности 10%ным раствором повидон-йода (Бетадин) более эффективно снижает бактериальную обсемененность кожи по сравнению с применением какого-либо одного антисептика [5].…”