Comparison of direct sequencing of the NS5B region with the Versant HCV genotype 2.0 assay for genotyping of viral isolates in Mexico

Simultaneous Hepatitis C Virus Genotyping and Variant Detection in Patients with Thalassemia: A Single-Center Phylogenetic Study

“…There were eight RASs to DAAs in nine isolates obtained from patients with thalassemia, of which five were in 1a, 3 in 1b, and 1 in 3a subtypes based on previous studies. 10 - 15 …”

“…8 Mutation analysis was performed based on the previous studies. [10][11][12][13][14][15] DISCUSSION: Global HCV subtypes prevalence in Iran included 1a as the predominant, followed by 3a. 16 There are different strategies for HCV genotyping.…”

“…There were eight RASs to DAAs in nine isolates obtained from patients with thalassemia, of which five were in 1a, 3 in 1b, and 1 in 3a subtypes based on previous studies. [10][11][12][13][14][15] The phylogenetic analysis could be used as a main tool for the study of rapidly-evolving RNA viruses such as HCV. HCV genetic evolution could be monitored as a useful tool for global surveillance and to develop efficient preventive and therapeutic strategies.…”

Full-genome next-generation sequencing of hepatitis C virus to assess the accuracy of genotyping by the commercial assay LiPA and the prevalence of resistance-associated substitutions in a Belgian cohort

“…Commercial tools in genotyping are generally benchmarked for their accuracy against Sanger sequencing of a small fragment of the NS5B gene (~350 bp) [5] due to its relatively high level of conservation across genotypes, simplifying primer design. While only 0.5% of LiPA analyses yield indeterminate results [6], studies estimating the concordance in interpretable genotypes of LiPA and NS5B sequencing, vary from as low as 80.4% to 100% [7][8][9][10][11][12]. Increasing sequence information for a sample beyond a single, conserved NS5B fragment of the HCV genome can contribute to more accurate genotyping using phylogenetic methods.…”