Comparison of Direct Immunofluorescence, Immunoassays, and Fecal Flotation for Detection of <i>Cryptosporidium</i> spp. and <i>Giardia</i> spp. in Naturally Exposed Cats in 4 Northern California Animal Shelters

Mekaru, Sumiko R.; Marks, Stanley L.; Felley, Allison; Chouicha, Nadira; Kass, Philip H.

doi:10.1111/j.1939-1676.2007.tb03049.x

Cited by 77 publications

(4 citation statements)

References 27 publications

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“…Therefore, in the present study, cats with pasty stools (44%) were significantly ( p < 0.05) more likely to be infected than those that had firm stools (16%) ( Table 2 ). These findings agree with those of other studies in which G. duodenalis was detected more frequently from animals with diarrhea [ 53 , 54 , 55 ].…”

Section: Discussionsupporting

confidence: 93%

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Veyna-Salazar

Cantó-Alarcón

Olvera-Ramírez

et al. 2023

Animals

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Giardia is a protozoan that affects humans as well as a wide range of domestic species. It is distributed worldwide, and the highest frequency is seen in developing countries. Due to the potential for domestic cats to be carriers of this parasite and subsequently transmit the infection to humans, it is important to know the risk of transmission. For this reason, the objective of this study was to determine the frequency of this parasite in the cat population of the city of Santiago de Queretaro, Mexico, and identify the assemblages present to determine the role this host plays in public health, this being the first study of its type to be performed in the country. This was a cross-sectional study during which 200 fecal samples were collected from cats of both sexes and varying ages and strata of origin. The samples were analyzed by microscopy following the flotation technique, having obtained a general frequency of 25%. Giardia cysts were found at higher frequency in pasty stools. The assemblages found were zoonotic, specifically assemblage A, which suggests that the cat poses an important risk for the dissemination of the parasite to humans, making it an important public health problem.

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Section: Discussionsupporting

confidence: 93%

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Veyna-Salazar

Cantó-Alarcón

Olvera-Ramírez

et al. 2023

Animals

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“…The highest sensitivity and specificity for Giardia spp. were detected for the immunoassay we utilized in our trial, when three different methods were compared [14]. We can therefore conclude that Giardia spp.…”

Section: Discussionmentioning

confidence: 97%

Effect of tylosin on dogs with suspected tylosin-responsive diarrhea: a placebo-controlled, randomized, double-blinded, prospective clinical trial

et al. 2011

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BackgroundThe macrolid antibiotic tylosin has been widely used to treat canine chronic diarrhea, although its efficacy is based on anecdotal reports and experimental studies in dogs and not on strong scientific evidence. The term tylosin-responsive diarrhea (TRD) refers to diarrheal disorders responding to tylosin therapy within a few days. In TRD, the stool remains normal as long as tylosin treatment continues, but diarrhea reappears in many dogs within weeks after discontinuation. The aim of our trial was to assess the effect of tylosin on fecal consistency compared with a placebo treatment in dogs with suspected TRD and additionally to establish whether tylosin in dogs with recurrent diarrhea is as effective as empirical studies and anecdotal reports suggest.MethodsSubjects comprised 71 client-owned dogs that, according to the owners, had previously been treated successfully with tylosin due to recurrent diarrhea of unknown etiology. At the initial examination, where there were no signs of diarrhea, the dogs were randomly assigned in a 2:1 ratio to a tylosin or placebo group. During a two-month follow-up the owners evaluated the fecal consistency according to previously published guidelines. When diarrhea recurred, either tylosin (25 mg/kg q 24 h, 7 days) or placebo treatment was initiated orally. Treatment outcome was evaluated as the mean of fecal consistency scores assigned during the last three days of the treatment period. To test for differences between the tylosin and placebo group in the proportion of responders, Pearson's Chi-squared test and Fisher's exact test were applied.ResultsSixty-one dogs met the selection criteria and were followed for two months. During the follow-up 27 dogs developed diarrhea and either tylosin or placebo treatment was started. The proportion of dogs with normal fecal consistency at the end of treatment was 85% (17/20) in the tylosin group and 29% (2/7) in the placebo group (Pearson's Chi-squared test p = 0.0049 and Fisher's exact test two-sided, p = 0.0114).ConclusionsOur findings indicate that tylosin is effective in treating recurrent diarrhea in dogs. The dose of 25 mg/kg once daily appears sufficient. No changes specific to TRD were detected in the examinations.

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“…The absence of these parasites in our results could be related to the low sensitivity of the adopted techniques. Considering Giardia spp., the combination of rapid tests and fecal flotation could reduce the chances of false negatives [37][38][39][40]. On the other hand, the diagnosis of Cryptosporidium spp.…”

Section: Discussionmentioning

confidence: 99%

Parasitosis in Pet Dogs from Rondônia, Amazon Biome, and Human Perception of Zoonoses

Mendonça,

Perin,

Zanini

et al. 2024

IJERPH

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Zoonotic parasitic diseases in dogs are particularly concerning in regions with low human development indices due to inadequate sanitary services and insufficient environmental and health education. This study aimed to assess the parasitological status of dogs living in households and evaluate their owners’ knowledge about zoonoses. A total of 183 dogs from Rolim de Moura, Rondônia State, were screened for the presence of ectoparasites, and 163 fecal samples were collected for analysis. The results showed that 74.23% (112/163) of the animals had at least one species of endoparasite. The most identified pathogens were Ancylostoma spp. (68.71%, 112/163), Trichuris vulpis (11.66%, 19/163), Toxocara canis (6.75%, 11/163), Cystoisospora canis (4.91%, 8/163), Dipylidium caninum (1.23%, 2/163), and Hammondia/Neospora (0.61%, 1/163). Ectoparasites were observed in 43.17% (79/183) of the evaluated animals, with Rhipicephalus sanguineus found in 31.15% (57/183) and Ctenocephalides felis felis in 20.77% (38/183). Only 11.48% (7/61) of the owners were familiar with the term “Zoonoses.” However, a significant majority (83.61%, 51/61) believed that dogs can transmit diseases to humans. Our findings highlight the prevalence of parasites in the studied area and associated risk factors, underscoring the urgent need for educational interventions to raise awareness about these diseases and their risks to human health.

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Comparison of Direct Immunofluorescence, Immunoassays, and Fecal Flotation for Detection of Cryptosporidium spp. and Giardia spp. in Naturally Exposed Cats in 4 Northern California Animal Shelters

Cited by 77 publications

References 27 publications

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Effect of tylosin on dogs with suspected tylosin-responsive diarrhea: a placebo-controlled, randomized, double-blinded, prospective clinical trial

Parasitosis in Pet Dogs from Rondônia, Amazon Biome, and Human Perception of Zoonoses

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