Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

Reis, Alexanda Dias; Fink, Maria Cristina Domingues da Silva; Machado, Clarisse Martins; Paz, José de Paula; Oliveira, Renato Reis; Tateno, Adriana Fumie; Machado, Adriana Freire; Cardoso, Maria Regina Alves; Pannuti, Cláudio Sérgio

doi:10.1590/s0036-46652008000100008

Cited by 30 publications

(23 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies comparing techniques of identifying RSV in samples in bronchiolitis have found that the most commonly used method of immunofluorescence is less sensitive (69–93%) than PCR (97–100%) 4 , 5. RSV negative bronchiolitis is therefore a diagnosis that is commonly accepted.…”

Section: Discussionmentioning

confidence: 99%

Not every cough in bronchiolitis season is bronchiolitis

Naidoo

Bryant²

2009

Case Reports

View full text Add to dashboard Cite

A 2-month-old male infant presented to hospital for the third time in late autumn with a 4 week history of cough and respiratory distress. He had presented to hospital on two occasions during the previous two weeks, and had twice been discharged with a diagnosis of bronchiolitis, based on clinical findings and the season. That the nasopharyngeal aspirate (NPA) was negative for respiratory viruses did not alter the diagnosis as this was felt to be a common false negative finding. However his cough worsened and on his third presentation he had respiratory distress with bilateral crackles and wheeze. The C reactive protein was 121 mg/l but the NPA was again negative for viruses. He was investigated for atypical causes of lower respiratory tract infection and the NPA was positive for Chlamydia trachomatis by polymerase chain reaction. He was treated with a 5 day course of azithromycin and made a full recovery.

show abstract

Section: Discussionmentioning

confidence: 99%

Not every cough in bronchiolitis season is bronchiolitis

Naidoo

Bryant²

2009

Case Reports

View full text Add to dashboard Cite

show abstract

“…Thus, the availability of a rapid and sensitive viral diagnostic assay is very important to establish appropriated therapeutic intervention, enabling physicians to make more accurate treatment decisions and reducing the use of unnecessary antibiotic therapy. Furthermore, rapid diagnosis favors the institution of specific precautions to minimize the risk of nosocomial infections 16 . In the last two decades, molecular techniques are improving, such as PCR (Polymerase Chain Reaction) or other amplification techniques of nucleic acid 11 .…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have evaluated the performance of in-house molecular techniques for diagnosis of respiratory viruses (RV) 2,16,19 . Commercial kits have also been evaluated and are considered rapid, sensitive and specific 12 .…”

Section: Introductionmentioning

confidence: 99%

Identification of respiratory virus in infants with congenital heart disease by comparison of different methods

Kanashiro

Boas

Thomaz

et al. 2011

Rev. Inst. Med. trop. S. Paulo

Self Cite

View full text Add to dashboard Cite

Respiratory virus infections are the main cause of infant hospitalization and are potentially severe in children with congenital heart disease (CHD). Rapid and sensitive diagnosis is very important to early introduction of antiviral treatment and implementation of precautions to control transmission, reducing the risk of nosocomial infections. In the present study we compare different techniques in the diagnosis of respiratory viruses in CHD infants. Thirty-nine samples of nasopharyngeal aspirate were obtained from CHD infants with symptoms of respiratory infection. The Multiplex PCR (Seeplex® RV 12 ACE Detection) driven to the detection of 12 respiratory viruses was compared with the direct immunofluorescence assay (DFA) and PCR, both targeting seven respiratory viruses. The positivity found by DFA, Multiplex and PCR was 33.3%, 51.3% and 48.7%, respectively. Kappa index comparing DFA and Multiplex, DFA and PCR and PCR and Multiplex PCR was 0.542, 0.483 and 0.539, respectively. The concordance between techniques was considered moderate. Both Multiplex PCR (p = 0.001) and PCR (p = 0.002) detected significantly more respiratory virus than DFA. As the performance of the tests may vary, the combination of two or more techniques may increase diagnostic sensitivity favoring the diagnosis of co-infections, early introduction of antiviral therapy and implementation of appropriate measures.

show abstract

“…To overcome this limitation, rapid techniques based on antigen detection by Direct Immuno Fluorescence (DIF) employing monoclonal antibodies is widely used for the diagnosis and subgroup of RSV into A and B [12]. Also Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) has been used increasingly to detect the virus in clinical samples [13]. The sensitivity of RT-PCR can equal or exceed that of cell culture or antigen-based assays [14].…”

mentioning

confidence: 99%

Respiratory Syncytial Virus Infection in Children

Zaghloul¹

2013

Air Water Borne Diseases

View full text Add to dashboard Cite

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

Cited by 30 publications

References 23 publications

Not every cough in bronchiolitis season is bronchiolitis

Not every cough in bronchiolitis season is bronchiolitis

Identification of respiratory virus in infants with congenital heart disease by comparison of different methods

Respiratory Syncytial Virus Infection in Children

Contact Info

Product

Resources

About