Comparison of Direct and Indirect cold atmospheric-pressure plasma methods in the B16F10 melanoma cancer cells treatment

Abstract

In this study a novel method was implemented and investigated in order to destroy cancer cells inside the mouse body on a clinical level. In the case of in-vitro study, MTT assay was employed to discover an effective dose of applied plasma and distinguish the plasma effect in direct and in indirect treatments. Tumor growth was also measured in in-vivo section so that the effectiveness of direct and indirect treatments could be compared. Furthermore, an investigation was conducted to study the interferences between a conventional method (chemotherapy) and plasma treatment so as to increase the effectiveness of treatment inside the body. Hematoxylin and Eosin, Flow Cytometry, TUNEL and Western Blot assay were used to investigate any cell alteration and the impact of various treatment methods on cancer cell and amount of their apoptosis and protein levels. Radiology and CT scan images were taken to determine the final tumor volume. The results showed a significant cell death and substantial reduction in tumor growth in direct plasma treatment in comparison with indirect plasma treatment. Eventually, dramatic destruction of cancer cells was observed while using of indirect plasma-chemotherapy combination, thus introducing an effective method for deep tissue tumors can be introduced.

Plasma-activated medium promotes autophagic cell death along with alteration of the mTOR pathway

“…Xiang L et al reported that PAM could selectively reduce the cell viability of triple negative breast cancer cells 23 . Cell death was also induced by treatment with PAM in other types of cancers, including pancreatic cancer, glioblastoma, squamous head and neck cancer, and melanoma [24][25][26] . These authors presumed that the underlying mechanism of cell death was related to increased apoptosis through the accumulation of intracellular reactive oxygen species (ROS).…”

Combination of Gas Plasma and Radiotherapy Has Immunostimulatory Potential and Additive Toxicity in Murine Melanoma Cells in Vitro

“…The half-life of many types of ROS is short, and the current concept is that it is not necessarily the plasma-derived ROS that are directly interacting with cells and tissues but rather secondary molecules derived from primary ROS as well as oxidative modifications on organic molecules [12]. When applied at higher doses, these ROS overcome the capacity of tumor cells and activate different cell signaling pathways that culminate in cell death [13,14]. Besides its cytotoxic effect, cold plasma has been described as a potent inducer of the immunogenic cancer cell death (ICD) [15,16].…”

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

“…1 The invention of the LASER (light amplification with stimulated emission of radiation) provided the opportunity to obtain high-power light at specific wavelengths and facilitated its application in biology and allowed a new field of research into the biological effects of radiation on animals and humans. 2 Low-level laser therapy (LLLT) was discovered in the 1960s and was first used by the National Aeronautics and Space Administration (NASA) in order to accelerate wound healing in the space. 3 PBM, or phot-modulation or a low-power laser is a non-invasive and non-toxic phototherapy that its wavelength is within the region of the red to near-infrared spectrum in the range of 600 to 1000 nm.…”