Comparison of Digestive Enzyme Activities and Expression of Small Intestinal Transporter Genes in Jinhua and Landrace Pigs

Liu, Xiuting; Lyu, Wentao; Liu, Lei; Lv, Kaikai; Zheng, Fuqiang; Wang, Yuanxia; Chen, Jinggang; Dai, Bing; Yang, Hua; Xiao, Yingping

doi:10.3389/fphys.2021.669238

Cited by 3 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The gastrointestinal digestive enzymes are involved in the regulation of growth and development of animals, because digestive enzymes can improve the feed efficiency by digestion and in turn to modulate the process of nutrient metabolism ( 46 ). Therefore, the activities and secretion of digestive enzymes in the small intestine are important indicators to evaluate intestinal development and digestive capacity in weaned pigs ( 38 , 47 ).…”

Section: Weaning Stress and Intestinal Developmentmentioning

confidence: 99%

Weaning stress and intestinal health of piglets: A review

Tang

Xiong²,

Fang³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Weaning is considered to be one of the most critical periods in pig production, which is related to the economic benefits of pig farms. However, in actual production, many piglets are often subjected to weaning stress due to the sudden separation from the sow, the changes in diet and living environment, and other social challenges. Weaning stress often causes changes in the morphology and function of the small intestine of piglets, disrupts digestion and absorption capacity, destroys intestinal barrier function, and ultimately leads to reduced feed intake, increased diarrhea rate, and growth retardation. Therefore, correctly understanding the effects of weaning stress on intestinal health have important guiding significance for nutritional regulation of intestinal injury caused by weaning stress. In this review, we mainly reviewed the effects of weaning stress on the intestinal health of piglets, from the aspects of intestinal development, and intestinal barrier function, thereby providing a theoretical basis for nutritional strategies to alleviate weaning stress in mammals in future studies.

show abstract

Section: Weaning Stress and Intestinal Developmentmentioning

confidence: 99%

Weaning stress and intestinal health of piglets: A review

Tang

Xiong²,

Fang³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…Intact intestinal barrier is necessary for animals to maintain nutrient absorption capacity [ 28 ], which is highly dependent on the length of the intestinal villus [ 29 ], the depth of the intestinal crypt [ 30 ], and the activity of digestive enzymes [ 31 ]. In this study, purslane significantly increased the villus height, crypt depth, and activity of intestinal digestive enzymes (α-amylase, lipase, and pepsin) in Chinese pond turtles infected with A. hydrophila .…”

Section: Discussionmentioning

confidence: 99%

Effect of Purslane (Portulaca oleracea L.) on Intestinal Morphology, Digestion Activity and Microbiome of Chinese Pond Turtle (Mauremys reevesii) during Aeromonas hydrophila Infection

Yang

Lin

Zhang

et al. 2023

IJMS

View full text Add to dashboard Cite

Large-scale mortality due to Aeromonas hydrophila (A. hydrophila) infection has considerably decreased the yield of the Chinese pond turtle (Mauremys reevesii). Purslane is a naturally active substance with a wide range of pharmacological functions, but its antibacterial effect on Chinese pond turtles infected by A. hydrophila infection is still unknown. In this study, we investigated the effect of purslane on intestinal morphology, digestion activity, and microbiome of Chinese pond turtles during A. hydrophila infection. The results showed that purslane promoted epidermal neogenesis of the limbs and increased the survival and feeding rates of Chinese pond turtles during A. hydrophila infection. Histopathological observation and enzyme activity assay indicated that purslane improved the intestinal morphology and digestive enzyme (α-amylase, lipase and pepsin) activities of Chinese pond turtle during A. hydrophila infection. Microbiome analysis revealed that purslane increased the diversity of intestinal microbiota with a significant decrease in the proportion of potentially pathogenic bacteria (such as Citrobacter freundii, Eimeria praecox, and Salmonella enterica) and an increase in the abundance of probiotics (such as uncultured Lactobacillus). In conclusion, our study uncovers that purslane improves intestinal health to protect Chinese pond turtles against A. hydrophila infection.

show abstract

“…A total of 60 newly born Jinhua pigs were fed in 6 pens, 10 pigs per pen, in a commercial pig facility with a standard corn-soybean-based diet and water ad libitum as described previously [ 12 ]. Within two months before slaughter, each pig was healthy and had not been treated with antibiotics.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Possible Link between the Gut Microbiome and Fat Deposition in Pigs

Zhao

Xiang

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Excessive lipid accumulation and high oxidative stress have become a serious health and economic problem in the pig industry. Fatness characteristics are crucial in pig production since they are closely related to meat quality. The gut microbiome is well acknowledged as a key element in fat deposition. But the link between gut microbiota and fat accumulation in pigs remains elusive. To examine whether there is a link between pigs’ gut microbiome, lipogenic properties, and oxidative stress, we selected 5 high-fat pigs and 5 low-fat pigs from 60 250-day-old Jinhua pigs in the present study and collected the colon content, serum sample, and liver and abdominal fat segments from each pig for metagenomic analysis, the oxidative stress assay, and RT-qPCR analysis, respectively. The backfat thickness and fat content of the longissimus dorsi muscle were considerably higher in the high-fat pigs than in the low-fat pigs ( P < 0.05 ). An obvious difference in GSH-Px and MDA in the serum between the high- and low-fat pigs was observed. After RT-qPCR analysis, we found the gene expression of ACC1 and SREBP1 in the liver and FAS, PPARγ, and LPL in the abdominal fat were significantly higher in high-fat pigs than in low-fat pigs ( P < 0.05 ). Additionally, metagenomic sequencing revealed that high-fat pigs had a higher abundance of Archaeal species with methanogenesis functions, leading to more-efficient fat deposition, while low-fat pigs had higher abundances of butyrate-producing bacteria species that improved the formation of SCFAs, especially butyrate, thus alleviating fat deposition in pigs. Furthermore, a total of 17 CAZyme families were identified to give significant enrichments in different fat phenotypes of pigs. This study would provide a detailed understanding of how the gut microbiome influences fat deposition in pigs, as well as a hint for improving growth performance and fatness traits by manipulating the gut microbiome.

show abstract

Comparison of Digestive Enzyme Activities and Expression of Small Intestinal Transporter Genes in Jinhua and Landrace Pigs

Cited by 3 publications

References 54 publications

Weaning stress and intestinal health of piglets: A review

Weaning stress and intestinal health of piglets: A review

Effect of Purslane (Portulaca oleracea L.) on Intestinal Morphology, Digestion Activity and Microbiome of Chinese Pond Turtle (Mauremys reevesii) during Aeromonas hydrophila Infection

Exploring the Possible Link between the Gut Microbiome and Fat Deposition in Pigs

Contact Info

Product

Resources

About