Comparison of different vaccines and induced immune response againstCampylobacter jejunicolonization in the infant mouse

Abstract

The degree of protection conferred by vaccinated dams on infant mice against colonization by Campylobacter jejuni depended on the bacterial strain, preparation, and route of administration of the vaccine. In some instances of homologous protection, serum bactericidal titres correlated well with protection. However, boiled C. jejuni vaccine, which was non-protective, also elicited a strong bactericidal antibody response. Conversely, bactericidal activity could not be demonstrated against strains capable of cross-protection. There was a good correlation between high campylobacter-specific IgG response and bactericidal activity.

Chronic Atrophic Gastritis in SCID Mice Experimentally Infected withCampylobacter fetus

“…In fact, when a normal fecal microbiota was introduced to ex-germfree mice monoassociated with Campylobacter, the Campylobacter species could no longer be cultured from the feces (33). This is in contrast to the majority of studies, in which mice with a normal fecal microbiota are persistently colonized with C. jejuni (1,7,25). In the present study we extended the previous studies of Campylobacter infection in immunocompromised mice by challenging outbred SCID mice colonized with a normal fecal microbiota with fresh clinical isolates of Campylobacter species.…”

“…They have been used to demonstrate the importance of several colonization factors in establishment of mucosal colonization (10,25). In addition, colonization models have been used to aid in the development of potential vaccines against C. jejuni (1,4,6). In other models, pretreatment of mice with iron gives lethality as a measurable end point following C. fetus infection (24).…”

Campylobacter jejuniinfection of infant mice: Acute enterocolitis is followed by asymptomatic intestinal and extra-intestinal immune responses

“…In the early days of Campylobacter research about three decades ago, neonatal mice had been used for investigating the role of flagella in the colonization of the intestine, the efficacy of bismuth subsalicylate in the prevention of colonization, the bacteriostatic effects of endotoxin and for vaccination strategies [20][21][22][23][24][25][26][27]. However, intestinal and extra-intestinal disease manifestations including clinical symptoms such as bloody diarrhea, histopathological changes, specific immune responses, and the complex microbiota have not been investigated so far.…”

“…Most importantly, weaned mice were shown earlier to be susceptible to C. jejuni infection. The corresponding models have been used successfully to study the impact of C. jejuni flagella, bismuth subsalicylate, and endotoxin in intestinal C. jejuni colonization, including potential vaccination strategies [20][21][22][23][24][25][26][27]. However, neither intestinal and extra-intestinal disease manifestation, nor specific immune responses and intestinal microbiota composition have been investigated in C. jejuni infected weaned mice so far.…”

Role of two flagellin genes in Campylobacter motility

“…While little is understood about the mechanisms by which campylobacters cause enteric disease, it has been established that the motility imparted by the polar flagellum is required for the establishment of infection in both experimental animal systems and in human volunteer studies (6,8,30). Moreover, flagellin is an immunodominant antigen of campylobacters, and data are accumulating suggesting that it may be a protective antigen (1,2,41).…”