Comparison of different techniques for gene transfer into mature and immature tobacco pollen

Stöger, Eva; Moreno, Rosa María Benito; Ylstra, Bauke; Vicente, Óscar; Heberle‐Bors, Erwin

doi:10.1007/bf02513024

Cited by 35 publications

(12 citation statements)

References 26 publications

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“…In general, the values in our present study are 1 order of magnitude higher than those reported with a pollen-specific PA2 promoter in tobacco (Stoger et al, 1992) and N. glutinosa (Plegt et al, 1992) pollen.…”

Section: Resultscontrasting

confidence: 54%

“…Note that the observed GUS activity with the CaMV 355 promoter did not differ greatly between lily and tobacco pollen. These values of GUS activity with the CaMV 35s promoter were smaller than those (1.4-1.9 pmol 4-MU h-' pg-' protein) reported for pollen grains of tobacco (Stoger et al, 1992) and Tradescantia (Hamilton et al, 1992). GUS activity in tobacco pollen observed here with the LAT52 promoter is about one-tenth of that observed with this promoter in pollen of transgenic tobacco plants (Twell et al, 1989a).…”

Section: Resultscontrasting

confidence: 40%

“…A similar difference between transient and stable expression of the GUS gene has been reported with the pollen-specific promoter PA2 in tobacco pollen; GUS activity (approximately 18 pmol 4-MU h" 1 ^g~' protein) detected by a transient expression assay in pollen into which the gene driven by the PA2 promoter was introduced (Stoger et al, 1992) is 5 to 10 times lower than the values (90-190 pmol 4-MU h" 1 /ig" 1 protein) in pollen of transgenic plants having the same construct (Plegt et al, 1992;Stoger et al, 1992). On the other hand, in the case of the CaMV 35S promoter the GUS activities in tobacco pollen as determined by transient assay (Stoger et al, 1992) and in transgenic plants (Twell et al, 1989a;Plegt et al, 1992;Stoger et al, 1992) were similar (1.5-4.2 pmol 4-MU h" 1 ^g~' protein). As reported previously (Yamashita et al, 1991), intracellular localization of gold particles introduced by bombardment is directly detected in cultured tobacco cells.…”

Section: Resultsmentioning

confidence: 96%

“…It has been reported that transient expression of the GUS gene driven by the CaMV 35s promoter is not detected histochemically in pollen grains of lily (Plegt et al, 1992) and N. glutinosa (Plegt et al, 1992) and is detected only rarely in those of tobacco (Twell et al, 1989a;Stoger et al, 1992) and Tradescantia (Hamilton et al, 1992). Also, in pollen of various transgenic plants including tobacco (Guerrero et al, 1990;Plegt et al, 1992), tomato (Plegt et al, 1992), and petunia (Mascarenhas and Hamilton, 1992), the expression of the GUS gene driven by the CaMV 35s promoter has not been detected histochemically.…”

Section: Resultsmentioning

confidence: 99%

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Expression of the [beta]-Glucuronidase Gene in Pollen of Lily (Lilium longiflorum), Tobacco (Nicotiana tabacum), Nicotiana rustica, and Peony (Paeonia lactiflora) by Particle Bombardment

et al. 1993

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Section: Resultscontrasting

confidence: 54%

Section: Resultscontrasting

confidence: 40%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Expression of the [beta]-Glucuronidase Gene in Pollen of Lily (Lilium longiflorum), Tobacco (Nicotiana tabacum), Nicotiana rustica, and Peony (Paeonia lactiflora) by Particle Bombardment

et al. 1993

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“…Fluorimetrical and histochemical GUS assays were carried out as described by Jefferson (22) and Stoger et al. (23). Under the conditions used, no endogenous GUS activity was detected in either assay (ref.…”

Section: Methodsmentioning

confidence: 99%

Pollen selection: a transgenic reconstruction approach.

Touraev

Fink²,

Stöger³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

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A transgenic reconstruction experiment has been performed to determine the feasibility of male gametophytic selection to enhance transmission of genes to the next sporophytic generation. For tobacco pollen from a transgenic plant containing a single hygromycin-resistance (hygromycin phosphotransferase, hpt-) gene under control of the dc3 promoter, which is active in both sporophytic and gametophytic tissues, 3 days of in vitro maturation in hygromycincontaining medium was sufficient to result in a 50% reduction of germinating pollen, as expected for meiotic segregation of a single locus insert. Pollination of wild-type plants with the selected pollen yielded 100%o transgenic offspring, as determined by the activity of the linked kanamycin-resistance gene-present within the same transferred T-DNA bordersunder control of the nos promoter. This is direct proof that selection acting on male gametophytes can be a means to alter the frequency of genes in the progeny.The plant life cycle consists of two distinct phases, the diploid sporophytic"and the haploid gametophytic phase. In angiosperms, the haploid phase is reduced, and the male and female gametophytes are enclosed in the respective sex organs of the sporophyte. Male and female gametophytes (i.e., pollen and embryo sacs) are formed from haploid spores, the direct products of meiosis, and the sporophytic phase starts again after the fusion of the two pollen-contained sperms with the egg and polar cells of the embryo sac in a double fertilization event. In spite of the large morphological and physiological differences, a large overlap of gene expression has been demonstrated between the sporophytic and gametophytic phase on the protein and mRNA level (for reviews, see refs. 1 and 2).These data provided the theoretical basis for the early pollen selection experiments (for review, see ref.2). The idea was that if such an overlap exists, it should be possible to exert selection pressures on pollen to increase the frequency of responsive genes in the subsequent sporophytic generation. Angiosperm pollen has some features that makes pollen selection a very attractive method of plant breeding: large population size, haploid nature of the cells, and independence from the maternal plant (3).A variety of selection pressures such as drugs, heat, water, cold temperature, and other stresses have been applied to mature pollen just before pollination, and nonstressed plants have been pollinated with the selected pollen in anticipation of obtaining progeny in which an increased frequency of resistant individuals can be found (for review, see ref. 4). Despite a few positive reports (5-9), the feasibility of pollen selection is still under question. This is mainly due to the problem of discriminating between gametophytic and sporophytic effects on pollen development (resulting from pollen-and antherexpressed genes, respectively; refs. 2 and 9) and a lack of appropriate selection schemes. The developmental windowThe publication costs of this article were defrayed in part by...

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