Comparison of different signal peptides for secretion of heterologous proteins in fission yeast

Kjærulff, Søren; Jensen, Michael

doi:10.1016/j.bbrc.2005.08.195

Cited by 38 publications

(18 citation statements)

References 34 publications

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“…However, yeasts do not secrete proteins in the same range of titers as filamentous fungi do, and it has been estimated that yeast’s secretory expression is 100‐ to 1000‐fold lower than the theoretical protein yield . Several strategies have been employed to increase the protein product available for secretion in S. cerevisiae including: (i) engineering of the peptide leader sequence, (ii) optimization of the gene copy number, (iii) manipulation of promoter strength, and (iv) engineering of the heterologous protein or gene of interest including codon bias optimization . These approaches have been combined with non‐rational strategies, such as (i) fermentation optimization and (ii) strain breeding approaches or with rational secretory pathway optimization strategies to circumvent protein processing and transit bottlenecks.…”

Section: Rational Engineering Of the Secretory Pathway For Enhanced Smentioning

confidence: 99%

Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

Kroukamp

Haan

Zyl

et al. 2017

Biofuels Bioprod Bioref

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Lignocellulosic biomass remains an attractive feedstock for the production of fuels if a technology can be developed to overcome its recalcitrance. Consolidated bioprocessing (CBP) is one technology under development that aims to make this conversion process economically feasible. While no ideal CBP organism has been developed, several options have been pursued including engineering of the ethanologenic yeast Saccharomyces cerevisiae. Considering the genetic malleability of this model organism, a variety of chemicals and chemical precursors could also be produced directly from cellulosic feedstocks, assuming an enzymatic system for the hydrolysis of the feedstock sugar polymers can be established. While there have been several accounts of the secretion of cellulases by strains of S. cerevisiae and the successful conversion of limited amounts of amorphous and model crystalline cellulose feedstocks to ethanol, substantial conversion of crystalline cellulose by these strains in the absence of exogenous cellulases has not been reported. The most cited reasons for this were the low secretion titer of cellulases in general and of cellobiohydrolases in particular. This review will compare the efforts that have been made to enhance heterologous protein secretion in the yeast S. cerevisiae through rational strain engineering with a focus on cellulases and will investigate important factors in developing successful CBP-yeast strains.

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Section: Rational Engineering Of the Secretory Pathway For Enhanced Smentioning

confidence: 99%

Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

Kroukamp

Haan

Zyl

et al. 2017

Biofuels Bioprod Bioref

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show abstract

“…Interestingly, we found that although the SP hlyA and SP vah3 delivery systems could not function well in secretion of GAPDH, they showed outstanding abilities to support secretion of another protective antigen (AopD of A. hydrophila) in V. anguillarum MVAV6203 (data unpublished). Similar phenomenon also occurred in the heterologous proteins secretion or display in different bacterial hosts (Kjarulff and Jensen, 2005;Xu et al, 2008;Yang et al, 2008a,b) and the mechanistic basis for this variation is largely unknown. All these results implied that the capability of a certain delivery system to direct secretion of different proteins might vary greatly.…”

Section: Discussionmentioning

confidence: 85%

A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila

Zhou

Wang

Liu

et al. 2010

Journal of Biotechnology

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“…Therefore, in order to further boost the export of BSH, it is necessary to test many other signal peptides of Tat pathway to find a good combination between the signal peptide and target protein [28,29]. Increasing the positive charge of the n-region and/or hydrophobicity of the signal peptide will be another effective method for enhancing the secretion efficiency [27,30,31].…”

Section: Discussionmentioning

confidence: 99%

Periplasmic Export of Bile Salt Hydrolase in Escherichia coli by the Twin-Arginine Signal Peptides

Dong

Zhang

et al. 2015

Appl Biochem Biotechnol

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Bile salt hydrolase (BSH, EC 3.5.1.24) is considered as an ideal way with lower cost and less side effects to release the risk of coronary heart disease caused by hypercholesterolemia. As bile salt hydrolase from Lactobacillus plantarum BBE7 could not be efficiently exported by PelB signal peptide of the general secretory (Sec) pathway, three twin-arginine signal peptides from twin-arginine translocation (Tat) pathway were synthesized, fused with bsh gene, inserted into expression vectors pET-20b(+) and pET-22b(+), and transformed into four different Escherichia coli hosts, respectively. Among the 24 recombinant bacteria obtained, E. coli BL21 (DE3) pLysS (pET-20b(+)-dmsA-bsh) showed the highest BSH activity in periplasmic fraction, which was further increased to 1.21 ± 0.03 U/mL by orthogonal experimental design. And, signal peptide dimethyl sulfoxide reductase subunit DmsA (DMSA) had the best activity of exported BSH. More importantly, the presence of BSH in the periplasm had proven to be caused by the export rather than cell leakage. For the first time, we report the periplasmic expression of BSH by signal peptides from the Tat pathway. This will lay a solid foundation for the purification and biochemical characterization of BSH from the supernatant, and strategies adopted here could be used for the periplasmic expression of other proteins in E. coli.

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Comparison of different signal peptides for secretion of heterologous proteins in fission yeast

Cited by 38 publications

References 34 publications

Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila

Periplasmic Export of Bile Salt Hydrolase in Escherichia coli by the Twin-Arginine Signal Peptides

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