Comparison of Different Nisin Separation and Concentration Methods: Industrial and Cost-Effective Perspectives

Tafreshi, Say-yed Hesameddin; Mirdamadi, Saeed; Khatami, Shohreh

doi:10.1007/s12602-019-09607-9

Cited by 8 publications

(12 citation statements)

References 38 publications

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“…Three different ASS levels were tested: 20, 40 and 60%. These ASS levels of ammonium sulfate salts were chosen according to results already reported in the literature in terms of nisin purification and yields [ 20 , 26 , 31 ]. A volume of 2 L of CFS containing nisin was produced by fermentation of L. lactis UL 719 in whey culture medium as described earlier and was aliquoted into three solutions with a 200 mL volume disposed in 500 mL Erlenmeyer flasks.…”

Section: Methodsmentioning

confidence: 99%

“…However, these methods display a high cost and complex applications at larger scale and introduce compounds of regulatory concern and large volumes of effluents that make them not suitable for widespread nisin applications [ 19 ]. The purification of nisin by salting out is also another method reported in the literature [ 20 , 21 ]. Ammonium sulfate and sodium chloride are the most often used salts for nisin precipitation after culture media clarification (removing the cell) into a cell-free supernatant (CFS) [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…They are generally applied before chromatographic purification techniques such as expanded bed ion exchange, immunoaffinity chromatography or hydrophobic interaction chromatography [ 9 , 23 , 24 ]. Salting out is based on protein solubility in water, where proteins need a low concentration of salt ions in order to remain soluble in a solution [ 20 , 24 , 25 , 26 ]. The use of salting out for nisin purification was reported in multiple studies which used sodium chloride [ 27 , 28 , 29 , 30 ] and ammonium sulfate precipitation at different optimal salt saturation levels for nisin recovery [ 20 , 24 , 25 , 26 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…Salting out is based on protein solubility in water, where proteins need a low concentration of salt ions in order to remain soluble in a solution [ 20 , 24 , 25 , 26 ]. The use of salting out for nisin purification was reported in multiple studies which used sodium chloride [ 27 , 28 , 29 , 30 ] and ammonium sulfate precipitation at different optimal salt saturation levels for nisin recovery [ 20 , 24 , 25 , 26 , 31 ]. However, one of the main hurdles of salting out processes are the generation of significant amounts of saline effluents which represent an ecological and economical issue regarding their further treatment and disposal [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nisin Purification from a Cell-Free Supernatant by Electrodialysis in a Circular Economy Framework

Rulence,

Perreault,

Thibodeau

et al. 2023

Membranes

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Nisin, an antimicrobial peptide produced by Lactococcus lactis strains, is a promising natural preservative for the food industry and an alternative to antibiotics for the pharmaceutical industry against Gram-positive bacteria. Nisin purification is commonly performed using salting out and chromatographic techniques, which are characterized by their low yields, the use of solvents and the production of large volumes of effluents. In the present work, the purification of nisin from a cell-free supernatant (CFS), after the production of nisin by fermentation on a whey permeate medium, was studied using ammonium sulfate precipitation and electrodialysis (ED) as a promising eco-friendly process for nisin purification. Results showed an increase in nisin precipitation using a 40% ammonium sulfate saturation (ASS) level with a purification fold of 73.8 compared with 34.5 and no purification fold for a 60% and 20% ASS level, respectively. The results regarding nisin purification using ED showed an increase in nisin purification and concentration fold, respectively, of 21.8 and 156 when comparing the final product to the initial CFS. Nisin-specific activity increased from 75.9 ± 4.4 to 1652.7 ± 236.8 AU/mg of protein. These results demonstrated the effectiveness of ED coupled with salting out for nisin purification compared with common techniques. Furthermore, the process was noteworthy for its relevance in a circular economy scheme, as it does not require any solvents and avoids generating polluting effluents. It can be employed for the purification of nisin and the recovery of salts from salting out, facilitating their reuse in a circular economy.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nisin Purification from a Cell-Free Supernatant by Electrodialysis in a Circular Economy Framework

Rulence,

Perreault,

Thibodeau

et al. 2023

Membranes

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show abstract

“…After extraction, the next step involves saturation, purification, and concentration, which are crucial in the purification stage of downstream processing [ 101 ]. The typical purification methods include chemical extraction, salt precipitation, membrane separation and chromatography [ 65 ].…”

Section: Application Of Ms In Amps Studymentioning

confidence: 99%

Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications

Fang,

Yu,

et al. 2024

Heliyon

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Comparison of Different Nisin Separation and Concentration Methods: Industrial and Cost-Effective Perspectives

Cited by 8 publications

References 38 publications

Nisin Purification from a Cell-Free Supernatant by Electrodialysis in a Circular Economy Framework

Nisin Purification from a Cell-Free Supernatant by Electrodialysis in a Circular Economy Framework

Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications

Purification, characterization, and mode of action of Sakacin ZFM225, a novel bacteriocin from Lactobacillus sakei ZFM225

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