Comparison of Different Methods for Separation of Haploid Embryo Induced through Irradiated Pollen and Their Economic Analysis in Melon (Cucumis melovar.inodorus)

Abstract

Irradiated pollen technique is the most successful haploidization technique within Cucurbitaceae. After harvesting of fruits pollinated with irradiated pollen, classical method called as “inspecting the seeds one by one” is used to find haploid embryos in the seeds. In this study, different methods were used to extract the embryos more easily, quickly, economically, and effectively. “Inspecting the seeds one by one” was used as control treatment. Other four methods tested were “sowing seeds direct nutrient media,” “inspecting seeds in the light source,” “floating seeds on liquid media,” and “floating seeds on liquid media after surface sterilization.” Y2 and Y3 melon genotypes selected from the third backcross population of Yuva were used as plant material. Results of this study show that there is no statistically significant difference among methods “inspecting the seeds one by one,” “sowing seeds direct CP nutrient media,” and “inspecting seeds in the light source,” although the average number of embryos per fruit is slightly different. No embryo production was obtained from liquid culture because of infection. When considered together with labor costs and time required for embryo rescue, the best methods were “sowing seeds directly in the CP nutrient media“ and ”inspecting seeds in the light source.”

Use of chemical mutagens for production of inactive pollen grains,embryo rescue, and morphological changes in cucumber

“…While it seems impossible to produce 100% homozygote plants by open pollination, in vitro techniques have marked a period of reform in obtaining pure lines over short spans of time. One of the fastest and most effective methods is the production of doublehaploid plants (Baktemur et al, 2013), for which two laboratory procedures have been developed and are now used commonly. The first consists of using either an ovary or an ovule culture.…”

“…However, these factors may affect ovule and ovary structure. The use of irradiated pollen has proved to be the most successful haploidization technique in Cucurbitaceae (Baktemur et al, 2013). After harvesting of fruits that were pollinated with irradiated pollen, the classical method of "inspecting the seeds one by one" is used to find haploid embryos in the seeds (Baktemur et al, 2013).…”

“…This technique resulted in haploid embryo production. Baktemur et al (2013) compared the various techniques of separation of haploid embryos (inspecting the seeds one by one, sowing seeds directly in nutrient media, and inspecting seeds in a light source), which were subsequently optimized for cucurbits by Navratilova et al (2011). Despite its limitations, parthenogenesis is routinely used in cucumber breeding procedures to achieve complete homozygosity in one generation (Claveria et al, 2005).…”

Production of Doubled Haploids in cucumber

“…В этой работе были вскрыты основные проблемы, характерные для этого семейства и предложены некоторые пути их возможного решения. Способ отделения гаплоидов из флотирующих на поверхности воды семян, предложенный Aalders, до сих пор успешно используется с различными модификациями, как у огурца, так и у других культур семейства Тыквенные (Lofti, Salehi, 2008;Baktemur et al, 2013).…”

“…Lofti M. и Salehi S. в 2008 году провели исследование на образцах дыни, в котором сравнили трудозатраты по четырем основным методам, использующимся для обнаружения зародышей: «проверка серии семян один за другим под стереомикроскопом», «прямой посев семян в питательные среды», «осмотр семян под флуоресцентным источником света», «плавающие семена на жидких средах» и «плавающие семена на жидких средах после поверхностной стерилизации». При рассмотрении экономической эффективности (трудозатраты, затраченное время, микробные загрязнения, сохранность эмбриоидов) наилучшими методами оказались «посев семян непосредственно в питательную среду CP (Chee et al, 1992)» и «осмотр семян под флуоресцентным источником света» (Baktemur et al, 2013).…”