Comparison of different methods for determining lignin concentration and quality in herbaceous and woody plant residues

Faust, Sibylle; Kaiser, Klaus; Wiedner, Katja; Glaser, Bruno; Joergensen, Rainer Georg

doi:10.1007/s11104-018-3817-0

Cited by 9 publications

(9 citation statements)

References 54 publications

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“…Lignin links with cellulose, hemicellulose and pectin, providing mechanical strength, especially in woody species. Moreover, lignin impedes digestion by herbivores, protects plants against pathogens and reduces lodging as well as the speed of decomposition (Faust et al ., 2018 ). It also plays a role in the stress response of plants to low temperature, drought, and salinity (Q. Liu et al ., 2018 ).…”

Section: Root Chemistrymentioning

confidence: 99%

“…Despite being relatively simple and reproducible, these methods generally lead to overestimation of the true lignin fraction in plant samples (Preston & Trofymow, 2015 ). The residue referred to as Klason lignin, ADL‐lignin and CHN‐lignin may contain proteins precipitated during earlier extraction steps (this is corrected for in the CHN‐lignin method) and other polymeric compounds such as waxes, suberin or condensed tannins, all of which resist acid hydrolysis, as well as nonsoluble compounds such as silicates (Faust et al ., 2018 ). In all of these gravimetric approaches, it is good to estimate and correct for the mineral fraction (including silicates) in the residue by determining the ash content, especially for roots as contamination by soil minerals is likely.…”

Section: Root Chemistrymentioning

confidence: 99%

“…Here, we present two proximate methods (ADL, CHN), as well as a method to determine more directly the monolignols (CuO). Since these methods yield contrasting results (Faust et al ., 2018 ), we advise to explicitly refer to either of ADL‐lignin, CHN‐lignin and CuO lignin.…”

Section: Root Chemistrymentioning

confidence: 99%

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A starting guide to root ecology: strengthening ecological concepts and standardising root classification, sampling, processing and trait measurements

et al. 2021

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Section: Root Chemistrymentioning

confidence: 99%

Section: Root Chemistrymentioning

confidence: 99%

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A starting guide to root ecology: strengthening ecological concepts and standardising root classification, sampling, processing and trait measurements

et al. 2021

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“…The RD-N/root-N and RD-C/root-C ratios suggest a higher contribution of fine root residues to pea RD in comparison with treatments AD and A. More root residues must be combined with a higher fibre concentration 34 , 35 . This would explain why the presence of triticale roots also decreased the incorporation of RD-N and RD-C into the soil microbial biomass, due to increased recalcitrance.…”

Section: Discussionmentioning

confidence: 90%

Evidence of considerable C and N transfer from peas to cereals via direct root contact but not via mycorrhiza

Hupe

Naether

Haase

et al. 2021

Sci Rep

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Intercropping of legumes and cereals is an important management method for improving yield stability, especially in organic farming systems. However, knowledge is restricted on the relevance of different nutrient transfer pathways. The objective of the study was to quantify nitrogen (N) and carbon (C) transfer from peas to triticale by (1) direct root contact (= R), (2) arbuscular mycorrhizal fungi (AMF; = A), and (3) diffusion (= D). Pea (Pisum sativum cv. Frisson and P2) and triticale (Triticum × Secale cv. Benetto) plants as intercrop were grown for 105 days. Treatment ADR enabled all transfer paths between the two crops. Treatment AD with root exclusion enabled AMF and diffusion transfer between peas and triticale. Treatment A with a diffusion gap barrier only allowed AMF transfer. Pea plants were labelled every 14 days with a 13C glucose and 15N urea solution, using the cotton wick technique. Direct root contact resulted in the highest pea rhizodeposition and thus the largest absolute amounts of N and C transfer to triticale. Root exclusion generally changed composition of rhizodeposits from fine root residues towards root exudates. Pea plant-N consisted of 17% N derived from rhizodeposition (NdfR) in treatment ADR but only 8% in the treatments AD and A, independently of pea variety, whereas pea plant-C consisted of 13% C derived from rhizodeposition (CdfR), without pea variety and transfer path treatment effects. Averaging all transfer path treatments, 6.7% of NdfR and 2.7% of CdfR was transferred from Frisson and P2 to triticale plants. Approximately 90% of this NdfR was transferred by direct root contact from Frisson to triticale and only 10% by AMF, whereas only 55% of CdfR was transferred to triticale by direct root contact, 40% by AMF and 5% by diffusion. Similar percentages were transferred from mutant P2 to triticale. Root exclusion generally changed RD composition from fine root residues towards root exudates.

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“…2 The analytical methodology employed here does not capture this feature; however, lignin can be measured using several well-established protocols. 39 The leaves also tended to have a greater abundance of 3,4-xylose relative to the stalk, indicating a greater degree of arabinose branching. If these hypotheses are true, the more branched xylan structures in the top portions of the plant and leaves could be more easily extracted or digested due to their weaker association with the cellulose framework, thus also resulting in less cellulosic recalcitrance.…”

Section: ■ Results and Discussionmentioning

confidence: 94%

Glycomic Mapping of the Maize Plant Points to Greater Utilization of the Entire Plant

Couture

Castillo

et al. 2021

ACS Food Sci. Technol.

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The goal of food sustainability is possible if greater utilization of plants is achieved. In corn, only the kernels are currently used for human consumption; however, edible carbohydrates that may function as dietary fiber are present throughout the plant. A glycomic map of the maize plant was obtained providing a broad structural view of the carbohydrate distribution revealing that non-cellulosic material was present throughout. Newly developed rapid throughput liquid chromatography tandem mass spectrometry-based methods for analyzing monosaccharide and linkage compositions show unique structural features in the respective segments and parts of the plants from the roots to the tassel. The most abundant monosaccharides of the 14 that were monitored included glucose, xylose, and arabinose. Additionally, galactose, fructose, rhamnose, mannose, galacturonic acid, and glucuronic acid were found in lower abundances. The relative abundances of each monosaccharide varied with the parts of the plants. Linkage compositions also varied and provided further structural information that included the presence of polysaccharides such as xylans, starch, pectins, xyloglucans, arabinans, galactans, and β-glucans. The nonstructural carbohydrate components including the free mono-and disaccharides were also measured to provide a unique geographical map of their abundances. The glycomic map of corn would guide traditional plant breeding methods and new genome editing tools toward tissue-specific enhancements of carbohydrate polymers that have unique and specific functional utility.

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Comparison of different methods for determining lignin concentration and quality in herbaceous and woody plant residues

Cited by 9 publications

References 54 publications

A starting guide to root ecology: strengthening ecological concepts and standardising root classification, sampling, processing and trait measurements

A starting guide to root ecology: strengthening ecological concepts and standardising root classification, sampling, processing and trait measurements

Evidence of considerable C and N transfer from peas to cereals via direct root contact but not via mycorrhiza

Glycomic Mapping of the Maize Plant Points to Greater Utilization of the Entire Plant

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