Comparison of different lipid extraction procedures applied to three microalgal species

Gorgich, Malihe; Mata, Teresa M.; Martins, António A.; Branco-Vieira, Monique; Caetano, Nídia S.

doi:10.1016/j.egyr.2019.09.011

Cited by 42 publications

(15 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many mechanic and non-mechanic disruption methods have been developed and applied to rupture cell walls of various microalgae; the former include bead beating [ 125 ], grinding [ 126 ], ultrasonication [ 127 ], high-pressure homogenization [ 128 ] and expeller pressing [ 129 ], and the latter include repeated freeze–thaw [ 130 ], osmotic shock [ 131 ], microwave radiation [ 132 ] and enzymatic digestion [ 133 ]. These methods should also work for cell wall disruption of C. zofingiensis , though modifications may be needed due to differences in cell wall composition and rigidity between C. zofingiensis and other algae [ 134 ].…”

Section: Zofingiensis As a Promising Producer Of Lipids And Carotenoidsmentioning

confidence: 99%

The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis

Bai

Liu

2021

Biotechnol Biofuels

View full text Add to dashboard Cite

The algal lipids-based biodiesel, albeit having advantages over plant oils, still remains high in the production cost. Co-production of value-added products with lipids has the potential to add benefits and is thus believed to be a promising strategy to improve the production economics of algal biodiesel. Chromochloris zofingiensis, a unicellular green alga, has been considered as a promising feedstock for biodiesel production because of its robust growth and ability of accumulating high levels of triacylglycerol under multiple trophic conditions. This alga is also able to synthesize high-value keto-carotenoids and has been cited as a candidate producer of astaxanthin, the strongest antioxidant found in nature. The concurrent accumulation of triacylglycerol and astaxanthin enables C. zofingiensis an ideal cell factory for integrated production of the two compounds and has potential to improve algae-based production economics. Furthermore, with the advent of chromosome-level whole genome sequence and genetic tools, C. zofingiensis becomes an emerging model for studying lipid metabolism and carotenogenesis. In this review, we summarize recent progress on the production of triacylglycerol and astaxanthin by C. zofingiensis. We also update our understanding in the distinctive molecular mechanisms underlying lipid metabolism and carotenogenesis, with an emphasis on triacylglycerol and astaxanthin biosynthesis and crosstalk between the two pathways. Furthermore, strategies for trait improvements are discussed regarding triacylglycerol and astaxanthin synthesis in C. zofingiensis.

show abstract

Section: Zofingiensis As a Promising Producer Of Lipids And Carotenoidsmentioning

confidence: 99%

The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis

Bai

Liu

2021

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…However, different types of extractives hinder the cellulosic pulp production in different intensities. Polar extractives are easier to remove, but non-polar extractives lead to higher removal costs [72][73][74][75]. Therefore, the focus of clone selection for the production of cellulosic pulp must be taken into account for woods that have lower concentrations of extractives.…”

Section: Composition Of Wood Extractivesmentioning

confidence: 99%

Determination of the Chemical Composition of Eucalyptus spp. for Cellulosic Pulp Production

Vieira

Arriel

Zanuncio

et al. 2021

Forests

View full text Add to dashboard Cite

The chemical composition of wood is important to assess the quality of this raw material for the industry of cellulosic pulp production. The purpose of this work was to determine the chemical composition of Eucalyptus spp. grown for cellulosic pulp production. Ten Eucalyptus spp. clones with six years of age, located in the municipality of Itamarandiba, Minas Gerais, Brazil, were used. Quantification was obtained for extractives, monosaccharides, uronic acids, acetates, lignin, ash and the phenolic composition of the extracts. In average, clones showed around 2.7% extractives, with a predominance of polar compounds soluble in ethanol and water; 27.7% lignin and 0.3% ash. Glucose was the main sugar detected (64.2%), followed by xylose (19.3%). The main components of the extractives were steroids, fatty acids and aromatic acids, followed by smaller amounts of substituted alkanoic acids, fatty alcohols, glycerol derivatives and triterpenes. The ethanol–water extracts presented total phenol contents ranging from 321.4 to 586.6 mg EAG/g of extract, tannins from 28.1 to 65.1 mg catechin/g of extract and flavonoids from 73.6 to 256.9 mg catechin/g of extract. Clones with a higher holocellulose amount and a lower lignin content showed a higher potential for cellulosic pulp production. These findings are important for the development of high-quality wood based on important traits for the pulp and paper sector.

show abstract

“…Depending on the starting material and the desired product, we can choose from a long list of biomass extraction protocols and very often they start from a known procedure such as e.g., the Bligh & Dyer protocol for lipid extraction which has been in use for more than half a century. This and other chemical extraction methods are often adapted to very small samples obtained from experimental lab work where the application of chemicals is limited and easily controlled (Fiset et al, 2017; Gorgich et al, 2020; Ryckebosch et al, 2012). Such protocols are not suitable for extraction of lipids for feed or food purposes due to the use of solvents such as methanol and chloroform, and for large‐scale extraction we need alternatives which are both environmentally and economically sustainable.…”

Section: Advancing Biobased Resource Production In the Nordic Regionmentioning

confidence: 99%

Sustainable resource production for manufacturing bioactives from micro‐ and macroalgae: Examples from harvesting and cultivation in the Nordic region

Chauton

Forbord

Mäkinen

et al. 2021

Physiologia Plantarum

View full text Add to dashboard Cite

Micro-and macroalgae are a great and important source of raw material for manufacturing of bioactives and ingredients for food, feed, cosmetics, or pharmaceuticals. Macroalgae (or seaweeds) have been harvested locally from wild stocks in smaller volumes for a long time, and a production chain based on cultivated seaweed for the harvest of considerably larger amounts is in progress for several species.Microalgae and cyanobacteria such as Spirulina have been produced in "backyard ponds" for use in food and feed also for a long time, and now we see the establishment of large production plants to control the cultivation process and increase the production yields. There is also a shift from harvesting or cultivation centered in

show abstract

Comparison of different lipid extraction procedures applied to three microalgal species

Cited by 42 publications

References 18 publications

The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis

The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis

Determination of the Chemical Composition of Eucalyptus spp. for Cellulosic Pulp Production

Sustainable resource production for manufacturing bioactives from micro‐ and macroalgae: Examples from harvesting and cultivation in the Nordic region

Contact Info

Product

Resources

About