Comparison of Different Laboratory Methods in the Detection of Anti-dsDNA Antibodies and Their Diagnostic Utility

Dalgıç, Ceyda Tunakan; Gökmen, Emine Nihal Mete; Sin, Aytül

doi:10.25002/tji.2020.1157

Cited by 1 publication

(2 citation statements)

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“…95%).Therefore, the results of CLIFT and ANA-IB had a high correlation coefficient (p=0.002, r=0.90). 8 The Korean team of "Yang JY et al" evaluated the diagnostic performance of 6 commercial kits for anti-dsDNA detection including CLIFT and Immunoblot IB on 142 sera. The results of these two techniques were concordant in 53.8% of cases.…”

Section: Discussionmentioning

confidence: 99%

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Performance evaluation of immunoblot for the detection of anti-DNA antibodies compared to indirect immunofluorescence on Crithidia luciliae

MAHIR¹,

HSAI²,

BOURHANBOUR³

et al. 2023

JHVRV

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Systemic lupus erythematosus is a severe autoimmune disease preferentially affecting the female sex. Because of the great polymorphism of the disease, the diagnosis of lupus must first be confirmed on the basis of several clinico-biological criteria. Among the biological criteria is the presence of anti-dsDNA (double stranded Deoxyribonucleic acid) antibodies. The detection of these autoantibodies requires the use of techniques with high sensitivity and specificity. We evaluated the performance of the immunoblot technique (ANA-IB) in comparison with the indirect immunofluorescence technique (CLIFT) in the detection of anti-dsDNA antibodies in 100 sera. This comparison led to the conclusion that CLIFT remains the reference method for the detection of these antibodies and cannot be replaced by ANA-IB. However, their concomitant use increases the specificity of the test.

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Section: Discussionmentioning

confidence: 99%

“…ELISA and immunoblot techniques are also used for verification and quantification. 8 In our study, we sought to compare CLIFT with Immunoblot to evaluate its performance in the detection of anti-dsDNA antibodies.…”

Section: Introductionmentioning

confidence: 99%