Comparison of different interpretation strategies for low template DNA mixtures

Pfeifer, Celine; Klein-Unseld, Rachel; Klintschar, Michael; Wiegand, Peter

doi:10.1016/j.fsigen.2012.06.006

Cited by 36 publications

(26 citation statements)

References 18 publications

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“…The four allele drop-ins were reexamined, but it was not possible to deduce whether the drop-ins were caused by a contamination or unusual high background noise in the electropherograms. The low drop-in rate of the sensitized SNPforID assay is in sharp contrast to the sensitized STR assays, where the drop-in rate may be 100 times higher [10][11][12][13][14][15][16][17][18]. Allele drop-ins are very problematic, because they may lead to false inclusions as well as false exclusions.…”

Section: Discussionmentioning

confidence: 99%

“…This indicated that the drop-ins were generated by the PCR and were not a result of contamination. By typing the samples two or more times and interpreting the combined results by various consensus models, the number of drop-outs and drop-ins in the generated sample profile were reduced, but not eliminated [10,12,15,16,18]. As a consequence, a ''statistical'' approach was developed to compensate for the stochastic phenomena of LtDNA analyses [2].…”

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confidence: 99%

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Forensic genetic SNP typing of low-template DNA and highly degraded DNA from crime case samples

Børsting¹,

Mogensen²,

Morling³

2013

Forensic Science International: Genetics

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Forensic genetic SNP typing of low-template DNA and highly degraded DNA from crime case samples

Børsting¹,

Mogensen²,

Morling³

2013

Forensic Science International: Genetics

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“…Two different statistical models were used aiming to obtain reliable results from the analysis of the Low Template-DNA (LT-DNA) from the swab samples: the "classical" biological model (Caragine et al 2009, Benschop et al 2011, Pfeifer et al 2012 and the statistical or probabilistic model (Curran et al 2005, Gill et al 2008, Gill and Buckleton 2009, Gill and Haned 2013.…”

Section: Discussionmentioning

confidence: 99%

Infanticide in brown bear: a case-study in the Italian Alps – Genetic identification of perpetrator and implications in small populations

Davoli¹,

Cozzo²,

Angeli³

et al. 2018

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Sexually Selected Infanticide (SSI) is thought of as a male reproductive strategy in social mammalian species, because females who lose cubs may quickly re-enter oestrus. SSI has rarely been documented in non-social mammals and, in brown bears, SSI has been studied mainly in an eco-ethological perspective. The authors examined the first genetically documented infanticide case which occurred in May 2015 in brown bears in Italy (Trentino, Central-Eastern Alps). The infanticide killed two cubs and their mother. Hair samples were collected from the corpses as well as saliva, through swabs on mother’s wounds, with the aim of identifying the genotype of the perpetrator. The samples were genotyped by PCR amplification of 15 autosomal microsatellite loci, following the protocol routinely used for individual bear identifications within the Interregional Action Plan for Brown Bear Conservation in the Central-Eastern Alps (PACOBACE). Reliable genotypes were obtained from the mother, cubs and putative perpetrator. The genotypes were matched with those populating the PACOBACE database and genealogies were reconstructed. Both mother and perpetrator genotypes were already present in the database. Kinship analyses confirmed mother-cubs relationships and identified the father of the cubs. In this study, for the first time, the authors used the open-source LRmix STUDIO software, designed to analyse human forensic genetic profiles, to solve a case in wildlife. Through LRmix STUDIO, those alleles that do not belong to the victims were isolated and, finally, the perpetrator was identified. This study presents a method that allows, through the application of different models, the genetic identification of the conspecific perpetrator with the highest probability. The identification of the infanticidal male is relevant for the better management and conservation of wild populations with small effective population size (Ne) and low population growth rate, especially in the case of recently established populations in human-dominated landscapes. This procedure will have predictably wide applications, supplying important data in the monitoring of small and isolated populations.

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“…It is well known that resolution of mixed samples after a-STR analysis is one of the main challenges which complicate routine and automated analysis of biological traces in forensics [11]. The main problems associated with mixtures are: the low amount of isolated DNA from forensic traces; inappropriate quality of isolated DNA; unknown number of contributors in the sample; contributor(s)' genotype composition; availability of reference samples; and proportion of minor contributor DNA [12][13][14][15][16]. In our study, fresh salivary or buccal swab samples of known contributors were analysed, thus some of the issues mentioned above were not applicable.…”

Section: Discussionmentioning

confidence: 99%

The Applicability of Autosomal Short Tandem Repeats Genotyping for Minor Contributor DNA Profiling from Mixed Saliva Samples

Repiská¹,

Durdiaková²,

Kamodyová³

et al. 2014

J Forensic

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Comparison of different interpretation strategies for low template DNA mixtures

Cited by 36 publications

References 18 publications

Forensic genetic SNP typing of low-template DNA and highly degraded DNA from crime case samples

Forensic genetic SNP typing of low-template DNA and highly degraded DNA from crime case samples

Infanticide in brown bear: a case-study in the Italian Alps – Genetic identification of perpetrator and implications in small populations

The Applicability of Autosomal Short Tandem Repeats Genotyping for Minor Contributor DNA Profiling from Mixed Saliva Samples

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