Comparison of different forms of dietary selenium supplementation on gene expression of cytoplasmic thioredoxin reductase, selenoprotein P, and selenoprotein W in broilers

Zhan, Xinqiao; Wang, H. F.; Yuan, Dong; Wang, Y. X.; Zhu, Fuge

doi:10.17221/7797-cjas

Cited by 14 publications

(8 citation statements)

References 52 publications

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“…A recent trial on the toxicity of Pb revealed that Se might alleviate the downregulation of GPX4, 2 and 1, DIO1, DIO2, TXNRD2-3, selenoprotein U, I, O, M, K, W, T, S 15 Sepx1, and Sepn1 expression in chicken cartilage tissue 81,82 . Similar results with Se-yeast and SeMet as organic Se sources upregulate GSH-Px1 and TXNDR1 mRNA expression in broiler breeders compared with sodium selenite 28,83 . Furthermore, SELW1 may participate in the protective role against H 2 O 2 , oxidative stress, and metabolic pathways 84,74,82 .…”

Section: Discussionsupporting

confidence: 69%

Effect of Organic and Inorganic Dietary Selenium Supplementation on Gene Expression in Oviduct Tissues and, Antioxidant Capacity of Laying Hens

Muhammad

Dalia

Loh

et al. 2020

Preprint

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Some functional genes were investigated for their involvement in egg (eggshell biomineralization) formation and selenoproteins in the oviduct and liver of laying hens fed different organic and inorganic selenium source. A total of 24 hens were selected randomly from the four treatments and slaughter. Uteri and liver tissue samples were collected from hens during the active growth phase of calcification (15 - 20 h post-ovulation) for RT-PCR. The basal diets supplemented with 0.3mg/kg of different organic Se sources and sodium selenite upregulate uterine and selenoproteins mRNA levels. This research reaps the advantage of tissue sampling from specialized segments of the oviduct that consecutively form different egg components. Expression of OC-17 and OC-116, and OC-17 were significantly higher in the uterus and magnum of laying hens, respectively. Their higher expression was observed with organic Se (bacterial selenoprotein or Se-yeast) fed-hens. The results may postulate the efficacy of organic Se in enhancing the expression of functional genes involved in the egg (eggshell biomineralization) formation and selenoproteins compared to inorganic and non-Se supplemented hens. This study proposed the efficacy of bacterial selenoprotein in the upregulation of the uterine genes and hepatic selenoproteins in laying hens.

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Section: Discussionsupporting

confidence: 69%

Effect of Organic and Inorganic Dietary Selenium Supplementation on Gene Expression in Oviduct Tissues and, Antioxidant Capacity of Laying Hens

Muhammad

Dalia

Loh

et al. 2020

Preprint

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show abstract

“…A recent trial on the toxicity of Pb revealed that Se might alleviate the downregulation of GPX4, 2 and 1, DIO1, DIO2, TXNRD2–3, selenoprotein U, I, O, M, K, W, T, S 15 SEPX1, and SEPP1 expression in chicken cartilage tissue [ 90 , 91 ]. Similar results with Se-yeast and SeMet as organic Se sources upregulate GPX1 and TXNRD1 mRNA expression in broiler breeders compared with sodium selenite [ 35 , 92 ]. Furthermore, SELW1 may participate in the protective role against H 2 O 2 , oxidative stress, and metabolic pathways [ 83 , 91 , 93 ], as comparable data were published in rat testes [ 94 ] and pig liver [ 67 ].…”

Section: Discussionsupporting

confidence: 53%

Effect of organic and inorganic dietary selenium supplementation on gene expression in oviduct tissues and Selenoproteins gene expression in Lohman Brown-classic laying hens

et al. 2021

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Background The oviduct of a hen provides a conducive environment for egg formation, which needs a large amount of mineral elements from the blood via trans-epithelial permeability. Eggshell is the calcified layer on the outside of an egg that provides protection and is critical for egg quality. However, little is known about the genes or proteins involved in eggshell formation, and their relationship to dietary microminerals. We hypothesized that dietary selenium supplementation in chickens will influence genes involved in eggshell biomineralization, and improve laying hen antioxidant capacity. The objective of this research was to investigate how organic and inorganic dietary selenium supplementation affected mRNA expression of shell gland genes involved in eggshell biomineralization, and selenoproteins gene expression in Lohman Brown-Classic laying hens. Results Shell gland (Uterus) and liver tissue samples were collected from hens during the active growth phase of calcification (15–20 h post-ovulation) for RT-PCR analysis. In the oviduct (shell gland and magnum) and liver of laying hens, the relative expression of functional eggshell and hepatic selenoproteins genes was investigated. Results of qPCR confirmed the higher (p < 0.05) mRNA expression of OC-17 and OC-116 in shell gland of organic Se hen compared to inorganic and basal diet treatments. Similarly, dietary Se treatments affected the mRNA expression of OCX-32 and OCX-36 in the shell gland of laying hens. In the magnum, mRNA expression of OC-17 was significantly (p < 0.05) higher in hens fed-bacterial organic, while OC-116 mRNA expression was down-regulated in dietary Se supplemented groups compared to non-Se supplemented hens. Moreover, when compared to sodium selenite, only ADS18 bacterial Se showed significantly (p < 0.05) higher mRNA levels in GPX1, GPX4, DIO1, DIO2 and SELW1, while Se-yeast showed significantly (p < 0.05) higher mRNA levels in TXNRD1 than the non-Se group. Conclusions Dietary Se supplementation especially that from a bacterial organic source, improved shell gland and hepatic selenoproteins gene expression in laying hens, indicating that it could be used as a viable alternative source of Se in laying hens. The findings could suggest that organic Se upregulation of shell gland genes and hepatic selenoproteins in laying hens is efficient.

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“…Our findings were also in agreement with those of [32] who reported that increased selenium levels had improved average daily gain in the same time there was no differences on average daily gain between nano-selenium and organic selenium in broiler chickens. The function of Se on growth rate may relate to its role in the selenoprotein P and selenoenzymes type I iodothyronine deiodinase expression, which have critical roles in the synthesis of thyroid hormones and Se transport [33]. Moreover, our results of increased growth performance with selenium methionine and nano-Se could possibly due to an increased thyroid hormone regulating the body’s energy metabolism and increased digestibility of protein [34].…”

Section: Resultsmentioning

confidence: 99%

Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens

Ibrahim

Kishawy

Khater

et al. 2019

Animals

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This study compares between different selenium forms (sodium selenite; SeS, selenomethionine; Met-Se or nano-Se) and levels on growth performance, Se retention, antioxidative potential of fresh and frozen meat, and genes related to oxidative stress in Ross broilers. Birds (n = 450) were randomly divided into nine experimental groups with five replicates in each and were fed diets supplemented with 0.3, 0.45, and 0.6 mg Se/kg as (SeS, Met-Se), or nano-Se. For overall growth performance, dietary inclusion of Met-Se or nano-Se significantly increased (p < 0.05) body weight gain and improved the feed conversion ratio of Ross broiler chicks at the level of 0.45 and 0.6 mg/kg when compared with the group fed the same level of SeS. Se sources and levels significantly affected (p < 0.05) its concentrations in breast muscle, liver, and serum. Moreover, Se retention in muscle was higher (p < 0.05) after feeding of broiler chicks on a diet supplemented with Met-Se or nano-Se compared to the SeS group, especially at 0.6 mg/kg. Additionally, higher dietary levels from Met-Se or nano-Se significantly reduced oxidative changes in breast and thigh meat in the fresh state and after a four-week storage period and increased muscular pH after 24 h of slaughter. Also, broiler’s meat in the Met-Se and nano-Se groups showed cooking loss and lower drip compared to the SeS group (p < 0.05). In the liver, the mRNA expression levels of glutathione peroxidase, superoxide dismutase, and catalase were elevated by increasing dietary Se levels from Met-Se and nano-Se groups up to 0.6 mg/kg when compared with SeS. Therefore, dietary supplementation with 0.6 mg/kg Met-Se and nano-Se improved growth performance and were more efficiently retained than with SeS. Both sources of selenium (Met-Se and nano-Se) downregulated the oxidation processes of meat during the first four weeks of frozen storage, especially in thigh meat, compared with an inorganic source. Finally, dietary supplementation of Met-Se and nano-Se produced acceptable Se levels in chicken meat offered for consumers.

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Comparison of different forms of dietary selenium supplementation on gene expression of cytoplasmic thioredoxin reductase, selenoprotein P, and selenoprotein W in broilers

Cited by 14 publications

References 52 publications

Effect of Organic and Inorganic Dietary Selenium Supplementation on Gene Expression in Oviduct Tissues and, Antioxidant Capacity of Laying Hens

Effect of Organic and Inorganic Dietary Selenium Supplementation on Gene Expression in Oviduct Tissues and, Antioxidant Capacity of Laying Hens

Effect of organic and inorganic dietary selenium supplementation on gene expression in oviduct tissues and Selenoproteins gene expression in Lohman Brown-classic laying hens

Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens

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