Comparison of different extraction techniques for the evaluation of polyphenols content in Summer savory extracts

Abstract

ABSTRACT. Efficiency of conventional solvent extraction (maceration and normal refluxing) and novel extraction techniques (ultrasound-assisted extraction-UAE and microwave-assisted extraction-MAE) were compared in order to obtain an enhanced content of total flavonoid, total phenolic compounds and rosmarinic acid from the Satureja hortensis L. (Summer savory) herb. Different mixtures of ethanol-water ratio were used for extraction of these compounds from dry plant material. High level of total flavonoid content was determined for a solvent system consisted of 40% respectively 50% ethanol (extraction systems ES6 and ES5) while the most efficient techniques were normal refluxing (R) and ultrasound-assisted extraction when sweep mode (UAE1) was selected. For the same solvent systems (ES6 and ES5), a high content of total phenolic compounds was determined when the normal refluxing (R) and microwave-assisted extraction (MAE1, with a duty coefficient of 40% and microwave action time1 min) were used for the extraction procedure. For the rosmarinic acid extraction, the maceration technique (M) combined with an ethanol-water system consisted of 60% ethanol (ES4) was found as the most efficient procedure.

Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff

“…Derivatives of HCA are common for plants belonging to the family Lamiaceae. Rosmarinic acid, is often one of the major polyphenol constituents in aerial parts polar extracts of these plants, including plants of the genus Satureja [28][29][30][31][32] . Besides rosmarinic acid, Lamiaceae species produce higher HCA oligomers which contain up to 8 monomer units 33,27 .…”

“…The majority (14) of eighteen identi ed avonoids, were avones, i.e. aglycons luteolin (29), apigenin (32), and genkwanin (34), as well as glycosides of luteolin (5, 8, 10, 11, 13, and 16) and apigenin (6 and 14) and their methylated derivatives (17, 23, and 26) (Table 1). The MS data obtained in the analysis of extracts and available standards, indicated that the sugar moieties of glycosides were either monosaccharide (hexose or hexuronic acid) or disaccharide units (composed of two hexuronic acids or deoxyhexose and hexose).…”

Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction

“…Derivatives of HCA are common for plants belonging to the family Lamiaceae. Rosmarinic acid is often one of the major polyphenol constituents in aerial parts of polar extracts of these plants, including plants of the genus Satureja [34][35][36][37][38] . Besides rosmarinic acid, Lamiaceae species produce higher HCA oligomers which contain up to 8 monomer units 33,39 .…”