Comparison of Different Extraction Solvent Mixtures for Characterization of Phenolic Compounds in Strawberries

Abstract

Eight different solvent mixtures containing acetone or methanol pure or combined with an acid (acetic, formic, hydrochloric) were tested for their efficiency for extraction of phenolic compounds from strawberries belonging to five groups of polyphenols: anthocyanins, flavonols, flavan-3-ols, hydroxycinnamic acid derivatives and conjugated forms of ellagic acid. Twenty-eight compounds from these five groups have been detected and quantified using HPLC-DAD-ESI-MS(n). The yield of each phenolic compound and group was evaluated with regard to the extraction solvent composition. Acetone containing extraction mixtures were superior to the ones containing methanol for extraction yield of total phenolic compounds, which was especially pronounced for the groups of flavan-3-ols and conjugated forms of ellagic acid. The mixture acetone/acetic acid (99:1, v/v) gave the best results for the qualitative and quantitative assay of the polyphenols present in strawberries since all 28 compounds were detected only in these extracts in quantities higher or comparable to the other extraction solvents tested.

Comparison of different extraction solvents for assay of the polyphenol content in the peel and pulp of 21 apple cultivars from Macedonia

“…On the other hand, poor water extraction results for the lyophilized apple samples, especially pulp, are most probably related to the content of sugars in the pulp, which causes difficulties in sample manipulation, especially in sample filtration (some of the sample is lost by sticking to filters and containers). In this manner, acetone, as a less polar solvent than water, was found to be more efficient, as has been demonstrated for the extraction of anthocyanins and fla-van-3-ols for strawberries by Kajdžanoska et al [17,18]. The charts also indicate more variable recovery of the water extracts in comparison with extracts obtained with methanol, acetone and the methanol/water mixture, which follow similar tendencies.…”

“…The results for total polyphenols obtained with acetone as the extraction agent show that the cultivars Cadel S3 (9.85 mg/g), Red Delicious S2 (11.85 mg/g), Kojce S14 (7.55 mg/g) and Mutsu S8 (8.22 mg/g) have a significantly higher concentration of polyphenols in the peel than in the pulp (Cadel 2.89 mg/g, Red Delicious 5.02 mg/g, Kojce 4.36 mg/g and Mutsu 4.46 mg/g). These cultivars are characterized by colored peel originating from anthocyanins, which are known to be extractable with acetone as solvent for higher molecular weight flavan-3-ols [17,18].…”

Solubility of Carvedilol in Ethanol + Propylene Glycol Mixtures at Various Temperatures

“…2 Solvent mixing is used in formulation of drugs, 3 crystal engineering and synthesis of cocrystals, 4 extraction, 5 and optimization of the mobile phase of highperformance liquid chromatography (HPLC) in pharmaceutical analysis. 6 In addition solvent mixing with temperature alteration is a common method to change the solubility of compounds in crystallization studies.…”

Phenolic Profile and Antioxidant Activity of Pulp and Peel from Peach and Nectarine Fruits

“…Within each plant species, the nature of those compounds can vary from organ to organ but is constant. These (Kajdžanoska et al, 2011). Various solvent systems have been used for the extraction of polyphenols from plant materials.…”