Comparison of Different Extraction and Detection Methods for Sugars Using Amino-Bonded Phase HPLC

Karkacıer, Mustafa; Erbaş, Mustafa; Uslu, Mustafa Kemal; Aksu, Mehmet

doi:10.1093/chromsci/41.6.331

Cited by 103 publications

(53 citation statements)

References 10 publications

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“…After cooling to 25 °C, the chromophore was measured at 520 nm by spectrophotometer (HACH DR/4000; Model 48000, HACH Company, Loveland, Colorado, USA) using L-proline as a calibration standard. Soluble sugar (sucrose, glucose and fructose) in the root, leaf and storage root tissues were assayed following the method of Karkacier et al (2003). In brief, fiftymilligram of plant sample was ground in a mortar with liquid nitrogen.…”

Section: Biochemical Assaymentioning

confidence: 99%

Physiological, Morphological Changes and Storage Root Yield of Sweetpotato [Ipomoea batatas (L.) Lam.] under PEG-Induced Water Stress

Yooyongwech

Samphumphung

Tisaram

et al. 2017

Not Bot Horti Agrobo

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Sweetpotato is an important tuberous root crop rich in nutrients such as vitamins and carbohydrates, and can grow well in arid regions with less water consuming crop. The aim of this research was to evaluate the storage root yields, physiological, biochemical and morphological traits in sweetpotato cv. 'Japanese Yellow' subjected to polyethylene glycol (PEG)-induced water deficit. At harvest (4 months after planting) the number of storage roots per plant and storage root fresh weight in sweetpotato treated with 5% PEG (−0.54 MPa) in nutrient solution of hydroponic culture declined by 20.0% and 47.4% compared to the control without PEG, respectively. Leaf area and leaf dry weight significantly decreased by 85.6% and 95.3%, respectively when exposed to water deficit stress. Sucrose content (114.7 mg g -1 dry weight; DW) in storage roots of sweetpotato grown under PEG-induced water deficit conditions was enriched by 2.2 fold of control (52.5 mg g -1 DW) and was greater than in storage roots derived from soil culture (70.3 mg g -1 DW). Total soluble sugar in the root and storage root tissues was enriched and may play a key role as osmotic adjustment (OA) in PEG-induced water stressed plants. Free proline and sucrose contents were also dominated in the leaf tissues to maintain the leaf osmotic potential in water stressed plants. In addition, chlorophyll degradation, chlorophyll fluorescence diminution and stomatal closure were found in plants grown under PEG-induced water deficit conditions, leading to reduction in net photosynthetic rate (Pn) and subsequently lesser amounts of glucose and fructose contents in the leaf tissues. Sucrose and free proline in the roots of sweetpotato play a key role as major osmotic adjustment when subjected to PEG-induced water deficit condition. Basic knowledge gained from this research will further be investigated the drought defense mechanism in sweetpotato via osmoregulation system.

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Section: Biochemical Assaymentioning

confidence: 99%

Physiological, Morphological Changes and Storage Root Yield of Sweetpotato [Ipomoea batatas (L.) Lam.] under PEG-Induced Water Stress

Yooyongwech

Samphumphung

Tisaram

et al. 2017

Not Bot Horti Agrobo

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“…Concentrations of soluble and insoluble starch were determined by colourimetric measurement of glucose residue after digestion using amyloglucosidase and α-amylase. Glucose and fructose in leaf blade tissues were analysed according to the modified method of Karkacier et al (2003). In a pre-cooled mortar, 100 mg fresh weight tissue was ground with liquid nitrogen, extracted with 1 mL nanopure water, vigorously shaken for 15 s, sonicated for 15 min and then centrifuged at 12,000 rpm for 15 min.…”

Section: Starch and Sugars Determinationmentioning

confidence: 99%

Transcriptional regulations of the genes of starch metabolism and physiological changes in response to salt stress rice (Oryza sativa L.) seedlings

Theerawitaya

Boriboonkaset

Cha–um

et al. 2012

Physiol Mol Biol Plants

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The aim of this investigation was to compare the transcriptional expression of starch metabolism, involving genes and physiological characters, in seedlings of two contrasting salt-tolerant rice genotypes, in response to saltstress. The soluble sugar content in rice seedlings of both salt-tolerant and salt-sensitive genotypes was enriched, relating to starch degradation, in plants subjected to 200 mM NaCl. In the salt-tolerant cultivar Pokkali, a major source of carbon may be that derived from the photosynthetic system and starch degradation. In starch degradation, only Pho and PWD genes in Pokkali were upregulated in plants subjected to salt stress. In contrast, the photosynthetic abilities of IR29 salt-susceptible cultivar dropped significantly, relating to growth reduction. The major source of carbohydrate in salt-stressed seedlings of the IR29 cultivar may be gained from starch metabolism, regulated by ADP-glucose pyrophosphorylase (AGP), starch synthase (SS), starch branching enzyme (SBE), starch debranching enzyme (ISA), glucan-water dikinase (GWD), dispropotionating enzyme (DPE), phospho glucan-water dikinase (PWD) and starch phosphorylase (Pho). Also, the major route of soluble sugar in salt-stressed Pokkali seedlings was derived from photosynthesis and starch metabolism. This was identified as novel information in the present study.

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“…Soluble sugars; sucrose, glucose and fructose, in both root and leaf tissues, were analyzed according to the modified Karkacier et al (2003) method. In a pre-cooled mortar, 100 mg of fresh weight tissues were ground with liquid nitrogen, extracted with 1 mL nanopure water, vigorously shaken for 15 s, sonicated for 15 min and then centrifuged at 12,000 rpm for 15 min.…”

Section: Biochemical Measurementsmentioning

confidence: 99%

Sugar accumulation, photosynthesis and growth of two indica rice varieties in response to salt stress

et al. 2008

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Sugar, a final product of photosynthesis, is reported to be involved in the defense mechanisms of plants against abiotic stresses such as salinity, water deficiency, extreme temperature and mineral toxicity. Elements involved in photosynthesis, sugar content, water oxidation, net photosynthetic rate, activity of enzyme and gene expression have therefore been studied in Homjan (HJ), salt-tolerant, and Pathumthani 1 (PT1), salt-sensitive, varieties of rice. Fructose-1,6-biphosphatase (FBP) and fructokinase (FK) genes were rapidly expressed in HJ rice when exposed to salt stress for 1-6 h and to a greater degree than in PT1 rice. An increase in FBP enzyme activity was found in both roots and leaves of the salt-tolerant variety after exposure to salt stress. A high level of sugar and a delay in chlorophyll degradation were found in salt-tolerant rice. The total sugar content in leaf and root tissues of salttolerant rice was 2.47 and 2.85 times higher, respectively, than in the salt-sensitive variety. Meanwhile, less chlorophyll degradation was detected. Salt stress may promote sugar accumulation, thus preventing the degradation of chlorophyll. Water oxidation by the light reaction of photosynthesis in the salt-tolerant variety was greater than that in the salt-sensitive variety, indicated by a high maximum quantum yield of PSII (F v /F m ) and quantum efficiency of PSII (U PSII ) with low nonphotochemical quenching (NPQ), leading to a high net photosynthetic rate. In addition, the overall growth performances in the salt-tolerant variety were higher than those in the salt-sensitive variety. The FBP gene expression and enzyme activity, sugar accumulation, pigment stabilization, water oxidation and net photosynthetic rate parameters in HJ rice should be further investigated as multivariate salt-tolerant indices for the classification of salt tolerance in rice breeding programs.

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Comparison of Different Extraction and Detection Methods for Sugars Using Amino-Bonded Phase HPLC

Cited by 103 publications

References 10 publications

Physiological, Morphological Changes and Storage Root Yield of Sweetpotato [Ipomoea batatas (L.) Lam.] under PEG-Induced Water Stress

Physiological, Morphological Changes and Storage Root Yield of Sweetpotato [Ipomoea batatas (L.) Lam.] under PEG-Induced Water Stress

Transcriptional regulations of the genes of starch metabolism and physiological changes in response to salt stress rice (Oryza sativa L.) seedlings

Sugar accumulation, photosynthesis and growth of two indica rice varieties in response to salt stress

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