Comparison of Different Egg Albumen Fractions as Sources of Ovomucin

Hiidenhovi, Jaakko; Mäkinen, Joni; Huopalahti, Rainer; Ryhänen, Eeva-Liisa

doi:10.1021/jf011333y

Cited by 30 publications

(20 citation statements)

References 36 publications

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“…The purity of the prepared ovomucin was determined using a high-load 16/60 column (Superdex 200 preparative grade, GE Healthcare Bio-Sciences AB, Uppsala, Sweden) coupled with fast-performance liquid chromatography equipment (AKTAexplorer, GE Healthcare Bio-Sciences AB) using the method as described by Hiidenhovi et al (2002) with slight modifications. Instead of using 2 Superose 6 columns in series in the original method, we used a Superdex 200 GFC matrix.…”

Section: Gel Filtration Chromatographymentioning

confidence: 99%

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Effect of different concentrations of calcium chloride and potassium chloride on egg white proteins during isoelectric precipitation of ovomucin

Omana

2009

Poultry Science

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The effect of various concentrations of CaCl2 and KCl on egg white proteins during isoelectric precipitation of ovomucin was investigated in this study. At low concentrations of CaCl2 (<50 mM), lysozyme was the major contaminant in the precipitated ovomucin, whereas ovalbumin was the predominant one at high concentrations (>or=100 mM). At 50 mM CaCl2 concentration, the concentrations of both lysozyme and ovalbumin were moderate. Ovomucin with a purity of 97.3% was prepared using a 2-step method: egg white was first precipitated in the presence of 50 mM CaCl2 followed by a second 500 mM CaCl2 extraction. The concentrations of other proteins in the precipitate were 1.3% of ovalbumin, 1.1% of lysozyme, and 0.4% of ovomucoid. Unlike CaCl2-treated samples, ovotransferrin was found to be the second major contaminant in all KCl-treated precipitates. Compared with the control, adding KCl at the lowest concentration of 2.5 mM increased significantly the content of ovalbumin (from 7.6 to 68.0%) and reduced significantly the content of lysozyme (from 25.5 to 6.4%) in the precipitates; however, increasing the concentrations of KCl up to 500 mM did not affect the content of ovalbumin, but the content of lysozyme showed a general reduction trend. Although KCl was used widely in literature as the last step of ovomucin washing, our results show that KCl is not an efficient salt in purifying ovomucin.

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Section: Gel Filtration Chromatographymentioning

confidence: 99%

“…The concentration of co-precipitated proteins was calculated from standard protein curves and the concentration of ovomucin was calculated by subtracting the amount of co-precipitated proteins according to Hiidenhovi et al (2002). All the determinations were carried out in duplicate.…”

Section: Gel Filtration Chromatographymentioning

confidence: 99%

Effect of different concentrations of calcium chloride and potassium chloride on egg white proteins during isoelectric precipitation of ovomucin

Omana

2009

Poultry Science

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“…In the state of being harmed by bacterial populations, OTf adjusts the levels of iron ions in the body [6]. …”

Section: Introductionmentioning

confidence: 99%

PC2 Ovotransferrin: Characterization and Alternative Immunotherapeutic Activity

Chiurciu

Oporanu

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Characterization and evaluation of immunotherapeutic potential of ovotransferrin PC2 (OTf PC2) were performed in this study. The ovoprotein was obtained from egg white from hens immunized with bacterial antigens, pathogenic for humans. For the negative control samples, OTf was extracted from eggs collected from Specific Pathogen-Free (SPF) hens and purified by affinity chromatography on Protein G-agarose column with two eluting peaks: I, representing ovalbumin, and II, ovotransferrin. The final apo-OTf form was reached by successive precipitation with ammonium sulfate and citric acid and the holo-OTf form by saturating the apo-form with FeCl3. Multiple OTf PC2 samples were analyzed through Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and, based on the molecular marker migration model, the ovotransferrin (76.5 kDa) and ovalbumin (45 kDa) were detected. The agglutination reaction exhibited statistically significant high specificity of the multiple OTf PC2, by reacting with the antigens used for hens' immunization. Following ELISA, it was established that OTf PC2 from hyperimmune eggs has specificity for all antigens; the antibody titer was high, indicating that OTf PC2 possesses immunological properties similar to immunoglobulin Y (Ig Y). This study suggests that OTf PC2 immunological activity may play a crucial role in the prevention and treatment of infections resistant to antibiotics and OTf PC2 can also act as a valuable nutraceutical.

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“…At present, OVN is most often isolated from egg white by isoelectric precipitation (pH 5.0–6.0), followed by a simple purification method that involves washing with sodium chloride salt solution, to remove co‐precipitating proteins like ovalbumin and lysozyme . It can also be solubilized by enzymatic hydrolysis and sonication in a mild alkaline solution or in a reducing agent such as mercaptoethanol …”

Section: Introductionmentioning

confidence: 99%

Evaluation of egg white ovomucin-based porous scaffold as an implantable biomaterial for tissue engineering

Carpena

Abueva

Padalhin

et al. 2016

J. Biomed. Mater. Res.

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Studies have shown the technological and functional properties of ovomucin (OVN) in the food-agricultural industry. But research has yet to explore its potential as an implantable biomaterial for tissue engineering and regenerative medicine. In this study we isolated OVN from egg white by isoelectric precipitation and fabricated scaffolds with tunable porosity by utilizing its foaming property. Gelatin a known biocompatible material was introduced to stabilize the foams, wherein different ratios of OVN and gelatin had a significant effect on the degree of porosity, pore size and stability of the formed hydrogels. The porous scaffolds were crosslinked with EDC resulting in stable scaffolds with prolonged degradation. Improved cell proliferation and adhesion of rat bone marrow-derived mesenchymal stem cells were observed for OVN containing scaffolds. Although, scaffolds with 75% OVN showed decrease in cell proliferation for L929 fibroblast type of cells. Further biocompatibility assessment as implant material was determined by subcutaneous implantation in rats of selected scaffold. H&E staining showed reasonable vascularization over time and little evidence of severe fibrosis at the implant site. Persistent polarization of classically activated macrophage was not observed, potentially reducing inflammatory response, and showed increased expression of alternatively activated macrophage cells that is favorable for tissue repair. Analysis of IgE levels in rat serum after implantation indicated minimal and resolvable allergic response to the OVN implants. The results demonstrate OVN as an acceptable implant scaffold that could provide new opportunities as an alternative natural biocompatible and functional biomaterial in various biomedical applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2107-2117, 2017.

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Comparison of Different Egg Albumen Fractions as Sources of Ovomucin

Cited by 30 publications

References 36 publications

Effect of different concentrations of calcium chloride and potassium chloride on egg white proteins during isoelectric precipitation of ovomucin

Effect of different concentrations of calcium chloride and potassium chloride on egg white proteins during isoelectric precipitation of ovomucin

PC2 Ovotransferrin: Characterization and Alternative Immunotherapeutic Activity

Evaluation of egg white ovomucin-based porous scaffold as an implantable biomaterial for tissue engineering

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