Comparison of Different Clean-Up Procedures for the Analysis of Deoxynivalenol in Cereal-Based Food and Validation of a Reliable HPLC Method

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

“…2, Scheme SI1). This result is in agreement with a previous report, which demonstrated that acetonitrile/water extraction led to a lower co-extraction of interfering matrix compounds than methanol/water solvent (Klötzel et al, 2005). The applicability of this immunosensor in breakfast cereal, already demonstrated with water extraction, was also confirmed with acetonitrile/water extraction; moreover, the use of a clean-up step did not lead to any significant improvement in respect of what obtained with unpurified extracts.…”

“…The need to comply with this regulation has increased the interest in the development of robust and reliable instrumental analytical methods and rapid test systems for in-field application. Commonly used methods for trichothecene detection include separating techniques coupled to different detectors (Klötzel et al, 2005;Lattanzio et al, 2009).…”

A new solid phase extraction clean‐up method for the determination of 12 type A and B trichothecenes in cereals and cereal‐based food by LC‐MS/MS

“…To compare the IAC (DONPrepm, R-Biopharm) and the Mycosepm method, we analyzed ten naturally contaminated samples with both methods [15,38] and determined the DON content by LC-MS/MS (Table 2). In all cases, we obtained significantly higher values (f = 2, p = 0.05%) using the IAC method.…”

“…Nevertheless, Mycosepm columns are at the moment the best compromise for a rapid and effective clean-up of crude extracts in the analysis of trichothecenes. In the last few years, the application of immunoaffinity columns (IAC) has been a great success for several mycotoxins [8,15]. This purification is highly specific and yields very good recovery rates, but is only applicable to one toxin at a time.…”

“…Alternatively, HPLC methods with UV, fluorescence or MS detection are used. HPLC methods with UV detection are only applicable to B-trichothecenes and require very effective clean-up procedures such as IAC [15]. For fluorescence detection, different pre-and post-column strategies have been established after derivatization with anthracene-or cumarinecarbonyl chlorides for A-trichothecenes and methylacetoacetate for type B trichothecenes [26 -29].…”