Comparison of different antigen preparations in an evaluation of the immune response toCampylobacter pylori

Hirschl, Alexander M.; Pletschette, M.; Hirschl, M. H.; Berger, Johannes; Stanek, Gerold; Rotter, M.

doi:10.1007/bf01962618

Cited by 59 publications

(29 citation statements)

References 26 publications

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“…The profile of the immune IgG response observed was different in each patient which agrees with previous reports on the existence of several major antigenic proteins in Helicobacter pylori (28)(29)(30)(31). In all six patients the immunoblot profiles remained similar, no qualitative changes being seen in the protein band patterns in sequential serum samples obtained after bacterial eradication.…”

Section: Immunoblot Analysis Using the Homologoussupporting

confidence: 92%

Effect of antimicrobial therapy on the specific serological response toHelicobacter pylori infection

Glupczynski

Burette

Goossens

et al. 1992

Eur. J. Clin. Microbiol. Infect. Dis.

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The systemic immune response to Helicobacter pylori was studied in 247 infected adult patients before antimicrobial therapy and at different intervals following therapy. Endoscopy with simultaneous collection of biopsies was performed in all patients immediately before treatment, 4 to 6 weeks after the end of therapy and 6 to 12 months later. A 14C-urea breath test was performed 3 to 6 months after the end of treatment. Biopsy specimens were cultured and examined histologically using Giemsa stain. Sera were tested for Helicobacter pylori IgG antibodies with a commercial enzyme immunoassay using species-specific antigens. Overall, Helicobacter pylori was eradicated in 120 patients while the other 127 remained infected with the organism. The follow-up period ranged from 4 weeks to 33 months (mean 10.2 months). Pretreatment IgG levels did not differ significantly between the two groups of patients. Six weeks after the end of treatment a slight but definite decrease in the IgG antibody levels was seen irrespective of treatment success. In the 127 patients who remained Helicobacter pylori-positive, the level of IgG antibodies remained stable or increased with time. A continuous fall in antibody levels was observed following bacterial eradication in the other 120 patients, but the difference in antibody levels between treatment responders and nonresponders became significant only more than six months after the end of treatment (p = 0.001). Serological testing may be useful for monitoring the outcome of long-term treatment of Helicobacter pylori infection and obviate the need for endoscopy.

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Section: Immunoblot Analysis Using the Homologoussupporting

confidence: 92%

Effect of antimicrobial therapy on the specific serological response toHelicobacter pylori infection

Glupczynski

Burette

Goossens

et al. 1992

Eur. J. Clin. Microbiol. Infect. Dis.

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“…Better discrimination was achieved with high-molecular-weight fractioned anti gen preparations. Among the crude antigen preparations, the sonicated whole cells and the ultracentrifuged sonicate discriminated better than the acid glycin extract [66]. A combination of different antigen preparations appeared to be superior in terms of sensitivity and specificity compared to the use of one antigen preparation alone [67],…”

Section: Imon-invasive Techniquesmentioning

confidence: 99%

A Review of Diagnostic Techniques for <i>Helicobacter pylori </i>Infection

Loffeld¹,

Stobberingh²,

Arends

1993

Dig Dis

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The description of Helicobacter pylori and an understanding of its pathogenetic significance has been one of the major breakthroughs in gastroenterology in the past decade. Because of the possible implications, a correct diagnosis of the infection is mandatory. Diagnostic techniques can be divided into invasive and non-invasive methods. The first require upper gastrointestinal endoscopy with a biopsy taken from the stomach. These biopsy specimens can be studied for the presence of H. pylori using several histological staining techniques, or culture. In addition, urease tests are possible. The non-invasive techniques do not require endoscopy. They comprise testing for the presence of antibodies against H. pylori in the patient’s serum, or breath tests with ¹³C- or ¹⁴C-labelled urea. In experienced hands every test has good sensitivity and specificity, and the test to be applied depends on local preference and experience.

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“…Early serological tests for H. pylori detection used intact cells or whole-cell lysates as antigens in the EIAs. Although the sensitivities of these tests were remarkably high, they lacked high specificity due to the presence of cross-reactivity with other bacteria (5,10,21). On the other hand, glycine-extracted cell surface proteins were reported to give less cross-reactivity with Campylobacter jejuni (18).…”

mentioning

confidence: 99%

Utilization of Proteinase K‐Treated Cells as Lipopolysaccharide Antigens for the Serodiagnosis of Helicobacter pylori Infections

Amano

Yokota

Ishioka

et al. 1998

Microbiology and Immunology

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We have evaluated the use of proteinase K (PK)-treated cells isolated from Helicobacter pylori as lipopolysaccharide (LPS) antigens in an immunoblot assay and an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of H. pylori infection. The sera from patients with chronic gastritis, gastric ulcer, duodenal ulcer or gastric cancer, and from healthy adults with or without H. pylori infection were assayed with three commercial serodiagnostic kits (HM-CAP, Helico-G, and G.A.P. II) and novel methods relying on the use of PK-treated cells. The PK-treated cells used in these assays were selected on the basis of their possibility to possess a common epitope in the 0-polysaccharides of H. pylori, which is known to be highly immunogenic in humans. Of the sera from these patients, 71-94% were positive with the commercial kits, 97 % with immunoblot assay, and 90% with ELISA. On the other hand, of the healthy adults infected with H. pylori, 72-97% were positive with the commercial kits, 86% with immunoblot assay, and 72% with ELISA. PK-treated cells that did not contain the common epitope were unsuitable as an antigen for immunoblot assay or ELISA. Furthermore, the reactivity of these sera reacted specifically with H. pylori PK-treated cells but not with LPSs from other gram-negative bacteria, such as Campylobacter, Proteus, Bordetella, and Salmonella. These results demonstrate that the serological assays relying on the use of H. pylori PK-treated cells possessing a highly antigenic epitope are potentially useful as a serodiagnostic test for H. pylori infection.

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Comparison of different antigen preparations in an evaluation of the immune response toCampylobacter pylori

Cited by 59 publications

References 26 publications

Effect of antimicrobial therapy on the specific serological response toHelicobacter pylori infection

Effect of antimicrobial therapy on the specific serological response toHelicobacter pylori infection

A Review of Diagnostic Techniques for <i>Helicobacter pylori </i>Infection

Utilization of Proteinase K‐Treated Cells as Lipopolysaccharide Antigens for the Serodiagnosis of Helicobacter pylori Infections

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