Comparison of dienogest and progesterone effects on uterine contractility in the extracorporeal perfusion model of swine uteri

Abstract

These results confirm the known inhibitory effect of progesterone on uterine contractility (relative to amplitude of contractions and overall contractility), affecting the whole organ. Perfusion of the uterus with dienogest also led to a general decrease in uterine contractility similar to the effect of progesterone.

The development of an extended normothermic ex vivo reperfusion model of the sheep uterus to evaluate organ quality after cold ischemia in relation to uterus transplantation

“…Earlier ex vivo uterus perfusion models have led to a significant contribution to our understanding of physiological events affecting fertility and gynecological disorders. 8,10,[16][17][18] These studies have also F I G U R E 6 No significant differences were seen between the groups at each time-point. However, there was a significant reduction in overall glucose and pH levels during the perfusion.…”

“…However, we did not perform any functional assessment of the perfused uteri, such as a contractility response following hormone stimulation. 13,14,18,20 This would have provided additional important information and should be considered in future experiments to aid in establishing a range of acceptable parameters/quality indicators in uteri undergoing reperfusion events. However, the primary goal of this study was to establish a highly reproducible ex vivo perfusion system and evaluate potential organ quality variances following different organ preservation settings.…”

“…Therefore, the development of the more reproducible ex vivo swine uterus perfusion model was less controversial, and has since been used extensively to study uterine physiological responses to hormones, [12][13][14][15] human semen 16 or various pharmacological agents. 17,18 For the majority of these generally short ex vivo experiments (<12 hours), the perfusate consisted of carbogen-oxygenated (95% oxygen and 5% carbon dioxide) Krebs-Ringer bicarbonate-glucose buffer. However, a modified Krebs-solution was used for the extended perfusion of the pig uterus (for times over 24 hours) with few negative metabolic events observed.…”

Normothermic Machine Perfusion Systems: Where Do We Go From Here?

“…124 Uterine NMP has been studied since the 1980s when Bulletti et al 125 attempted to perform embryo implantation after a total perfusion time of 52 h; however, ethical controversies prompted subsequent NMP studies to focus on uterine contractility and increased the usage of swine models. [126][127][128][129][130][131][132] These studies employed 4 to 27 h of perfusion and shared characteristics such as free drainage of the uterine veins and a perfusate without oxygen carriers. Perfused uteri presented modified contractibility profiles in response to hormones but increased lactate levels in the perfusate, probably due to inadequate oxygenation.…”

Uterus bioengineering as a future alternative to uterus transplantation

“…Additionally, novel normothermic organ perfusion systems using blood-based perfusion medium have proved better than conventional organ preservation methods for liver and kidney transplantation [60,61]. With modifications, these and other perfusion systems specifically developed for ex vivo pig, sheep and human uterus perfusion [62][63][64][65], will likely prove to be of significant value for future whole uterus recellularization protocols. During this extended in vitro recellularization time, multiple cell types will need to be applied in sequence, including endothelial cells for the vasculature reconstruction [66,67].…”