Comparison of detection methods for trichothecenes produced by fusarium sporotrichioides on fodder and grains at different air humidities

Abstract

Growth and toxin production of a highly toxic strain of Fusarium sporotrichioides Sherb were studied on oat and wheat grains and on straw under experimental conditions, in which relative humidity (RH) of air was regulated. The materials were incubated at three different RH levels at a range of 84-100%. F. sporotrichioides grew well on oat and wheat grains at RH 97-100% but grew less well at RH 84-88% and on straw. Toxin production was measured with three biological toxicity tests (cytotoxicity test, dermotoxicity test, and yeast cell toxicity test), with chemical analysis, and T-2 ELISA assay. Cytotoxicity and production of trichothecene mycotoxins were detected in all the samples incubated at all three RH levels. On oat and wheat grains, T-2 toxin, neosolaniol, and diacetoxyscirpenol were found, and on straw T-2 toxin, HT-2 toxin, neosolaniol, and T-2 tetraol were determined. In the T-2 ELISA assay, all material samples were found to contain T-2 toxin. The cytotoxicity test was the most sensitive method for detecting biological toxicity of samples inoculated with fungus. The T-2 ELISA assay and chemical analysis were about equally sensitive to detect T-2 toxin in samples.

Trichothecene Mycotoxins Activate Inflammatory Response in Human Macrophages

“…They were grown on rice flour agar as previously described (35,36). When grown on rice agar, S. chartarum strain 29 is not capable of satratoxin production (only minor amounts of stachybotrylactam and stachybotrytlactone are produced, Ͻ1 ng per 10 5 spores), whereas strain 72 produces satratoxin G and H, stachybotrylactone, and stachybotrylactam in the respective amounts of 4 ng, 10 ng, 8 g, and 2 g per 10 5 spores.…”

Indoor Mold, Toxigenic Fungi, andStachybotrys chartarum: Infectious Disease Perspective

“…Many purported fungal volatiles are in fact common and are not unequivocally fungal in origin (267). While some true mycotoxins have been detected in indoor air, this has usually been in the context of heavy industrial contamination (240,295). Although it is occasionally possible to collect mycotoxins by using air filters followed by extraction (318,388), they are usually isolated from inert dust or building materials (9, 79,111,267,294).…”

A rapid method with ultra‐high‐performance liquid chromatography–tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines

“…For quantitative analysis of type B trichothecenes in food and feedstuff, methods based on TLC, ELISA, GC and HPLC have been developed 16–22. However, poor separation and unsatisfying accuracy limit the application of TLC.…”