Comparison of desorption ionization methods for the analysis of neutral seven-coordinate technetium radiopharmaceuticals

Unger, Steve; McCormick, Terry J.; Treher, Elizabeth; Nunn, Adrian D.

doi:10.1021/ac00135a017

Cited by 13 publications

(2 citation statements)

References 15 publications

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“…show strong molecular ion peaks at m/z 668/670 (M + H) + or 666/668 (M -H)" in the positive and negative modes, respectively. Losses observed from these ions parallel fragmentation patterns observed previously in the spectra of several other BATO complexes (17). The infrared spectrum of TcC1(DMG)3CPITC contains a pronounced peak at about 2100 cm-1 due to the NCS group; strong carboxylate absorptions are seen at 3440 (vs) and 1720 cm-1 (m).…”

Section: Resultssupporting

confidence: 77%

Technetium-labeling of monoclonal antibodies with functionalized BATOs: 2. TcCl(DMG)3CPITC (CPITC=carboxyphenylisothiocyanate) labeling of B72.3 and NP-4 whole antibodies and NP-4 F(ab')2

Linder¹,

Wen²,

Nowotnik³

et al. 1991

Bioconjugate Chem.

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BATO (boronic acid adduct of technetium dioximes) complexes, TcCl(dioxime)3BR, were prepared in which the boron substituent (R) was the protein-reactive 2-carboxy-4-phenyl isothiocyanate (CPITC). The 99Tc complexes, where the dioxime was either dimethylglyoxime (DMG) or cyclohexanedione dioxime (CDO), were prepared and characterized. The 99mTc complex TcCl(DMG)3CPITC was prepared from a freeze-dried kit and used to label B72.3 (anti-TAG.72) and NP-4 (anti-CEA) whole antibodies, and the NP-4 F(ab')2 fragment. SDS-PAGE electrophoresis indicated that the labeling reagent was strongly bound to antibody. The labeled antibodies displayed high binding to affinity columns and good tumor uptake in GW39 tumor-bearing mice.

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Section: Resultssupporting

confidence: 77%

Technetium-labeling of monoclonal antibodies with functionalized BATOs: 2. TcCl(DMG)3CPITC (CPITC=carboxyphenylisothiocyanate) labeling of B72.3 and NP-4 whole antibodies and NP-4 F(ab')2

Linder¹,

Wen²,

Nowotnik³

et al. 1991

Bioconjugate Chem.

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“…Fast atom bombardment mass spectral (FAB-MS) fragmentation patterns for the nitroimidazole BATOs were similar to those observed for other BATOs (34). A molecular ion and protonated molecular ion were seen for all of the chloro compounds in the positive mode.…”

Section: Resultssupporting

confidence: 56%

Synthesis, characterization, and in vitro evaluation of nitroimidazole-BATO complexes: New technetium compounds designed for imaging hypoxic tissue

Ke¹,

Yw²,

Je³

et al. 1993

Bioconjugate Chem.

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Several technetium-99 BATO (boronic acid adduct of technetium dioximes) complexes TcX(dioxime)3BR (X = Cl) that contain a boron cap R which bears a 2- or 4-nitroimidazole moiety have been prepared from either TcCl(dioxime)3 or from Tc(dioxime)3(mu-OH)SnCl3 [dioxime = dimethyl glyoxime (DMG) or cyclohexanedione dioxime (CDO)]. Two hydroxy analogs (X = OH) were isolated by treatment of the corresponding chloro complexes with aqueous NaOH. The complexes have been characterized by elemental analysis, mass spectrometry, NMR, UV/vis spectroscopy, and high-performance liquid chromatography. These complexes have the potential for selective retention in hypoxic tissue, by a mechanism believed to be the result of nitro reduction. The electrochemistry and enzymatic reduction of these complexes was studied to assess the potential for reduction in vivo. The nitroreductase enzyme xanthine oxidase was shown to reduce the nitroimidazole group on the complexes 99TcOH(DMG)3BBNO2 and 99TcOH(DMG)3BprenNO2 under anaerobic conditions in the presence of hypoxanthine. However, the results indicated that the rate of reduction might be slow in vivo, limiting the suitability of these compounds for imaging of regions of hypoxia.

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Complex chemistry of technetium‐99

Schwochau

2000

Technetium

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Comparison of desorption ionization methods for the analysis of neutral seven-coordinate technetium radiopharmaceuticals

Cited by 13 publications

References 15 publications

Technetium-labeling of monoclonal antibodies with functionalized BATOs: 2. TcCl(DMG)3CPITC (CPITC=carboxyphenylisothiocyanate) labeling of B72.3 and NP-4 whole antibodies and NP-4 F(ab')2

Technetium-labeling of monoclonal antibodies with functionalized BATOs: 2. TcCl(DMG)3CPITC (CPITC=carboxyphenylisothiocyanate) labeling of B72.3 and NP-4 whole antibodies and NP-4 F(ab')2

Synthesis, characterization, and in vitro evaluation of nitroimidazole-BATO complexes: New technetium compounds designed for imaging hypoxic tissue

Complex chemistry of technetium‐99

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