Comparison of denitrification rate estimation techniques in a large, shallow lake

Messer, Jay J.; Brezonik, Patrick L.

doi:10.1016/0043-1354(83)90232-4

Cited by 58 publications

(19 citation statements)

References 14 publications

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“…These methods provide information on controlling environmental factors (Messer & Brezonik 1984), on spatial variation in rates within a lake, or on short-term temporal variation in rates (Keeney et al 1971;Chan & Campbell 1980;Messer & Brezonik 1983;Seitzinger 1988). Lake denitrification has also been estimated using the mass-balance approach, i.e.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen mass balances and denitrification rates in central Ontario Lakes

1993

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Abstract. Nitrogen mass balances for seven unproductive lakes and 20 forested catchments in central Ontario were measured between 1977 and 1989. Average annual lake denitrification rates calculated with the N/P ratio method were strongly correlated with summer anoxic factor (extent of surficial sediment anoxia) whereas denitrification rates calculated with a 210 Pb sediment N accumulation method were poorly correlated with the anoxic factor suggesting that the N/P method is superior. Substantial denitrification occurred in all lakes -an average of 36% of TN inputs or 75% of the net gain. On a regional area-weighted basis, 67% of bulk atmospheric TN deposition was stored or denitrified terrestrially, 12% was denitrified in lakes, 4% was stored in lake sediments, and 17% was exported from lakes. N/P ratios were generally less in streams than in precipitation suggesting preferential N retention in catchments, whereas the N/P ratios in lake outputs were slightly higher than lake input ratios, suggesting preferential P retention in lakes. This is consistent with the notion that P-limited lakes can exist adjacent to N-limited forests.

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Section: Introductionmentioning

confidence: 99%

Nitrogen mass balances and denitrification rates in central Ontario Lakes

1993

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“…Denitrification occurs in sediments in lakes (e.g. Andersen 1977; Keeney et al 1971;Messer & Brezonik 1983;Gardner et al 1987), rivers and streams (e.g. Chatarpaul et al 1980;Hill 1981;Billen et al 1985;Christensen & Sorensen 1988), estuaries and coastal bays (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…A few studies have concurrently used two or more techniques in an aquatic sediment and compared the resultant denitrification rates (Messer & Brezonik 1983;Smith & DeLaune 1983;Kemp et al 1990) but these studies have not generally been designed to specifically identify or clarify problems or limitations. Some studies in homogenized agricultural soil samples have compared denitrification methods (e.g.…”

Section: Introductionmentioning

confidence: 99%

Denitrification measurements in aquatic sediments: A comparison of three methods

Seitzinger¹,

et al. 1993

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Measurements of denitrification using the acetylene inhibition, 15N isotope tracer, and N, flux methods were carried out concurrently using sediment cores from Vilhehnsborg so, Denmark, in an attempt to clarify some of the limitations of each technique. Three experimental treatments of overlying water were used: control, nitrate enriched, and ammonia enriched water. The N, flux and 15N tracer experiments showed high rates of coupled nitrification/denitrification in the sediments. The acetylene inhibition method did not capture any coupled nitrification/denitrification. This could be explained by acetylene inhibition of nitrification. A combined 15N tracer/acetylene inhibition experiment demonstrated that acetylene inhibition of NzO reduction was incomplete and the method, therefore, only measured approximately 50% of the denitrification due to nitrate from the overlying water. Similar rates of denitrification due to nitrate in the overlying water were measured by the N, flux method and the acetylene inhibition method, after correcting for the 50% efficiency of acetylene inhibition. Rates of denitrification due to nitrate from the overlying water measured by the 15N tracer method, however, were only approximately 35% or less of those measured by the acetylene inhibition or N, flux methods.

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“…Techniques used in studies of nitrogen transformations include mass balance calculations (Messer & Brezonik 1983), flux measurements of relevant chemical species in whole core microcosms (Andersen et al 1984, tracer techniques (Rysgaard et al 1993, and microsensors , Jensen et al 1993, 1994. Studies of fluxes provide overall information about exchange of inorganic nutrients over the sediment surface, and by using isotopic tracers in such studies, it is possible to obtain detailed information about the individual processes taking place within the sediment.…”

Section: Introductionmentioning

confidence: 99%

Use of NOx- microsensors to estimate the activity of sediment nitrification and NOx- consumption along an estuarine salinity, nitrate, and light gradient

Kjær¹,

Revsbech²

2001

Aquat. Microb. Ecol.

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Microprofiles of nitrate plus nitrite (NO x -) were measured in sediment cores sampled at 6 stations along a nitrate and salinity gradient and a depth transect in Randers Fjord, Denmark. Rates of NO x -production and consumption were calculated from the concentration profiles to describe the variation of these processes under in situ conditions. A microscale biosensor for NO x -was used to obtain NO x -profiles in darkness and under in situ light conditions. This new tool for nitrate plus nitrite measurements in saline environments made it possible to measure NO x -profiles with submicromolar resolution and without interference from chemical species other than nitrous oxide. The NO x -concentration in the inner and middle part of the estuary was very high (55 to 220 µM). The range of NO x -consumption rates found here (47 to 577 µmol N m -2 h -1) were within the range of denitrification rates found in similar environments (measured by 15 N isotope techniques). The NO x -concentration in the water was low at the outermost part of the estuary, and all NO x -diffusing into the sediment was consumed in the oxic zone (top 1 mm) of the sediment. In this study, NO x -consumption was not closely coupled to nitrification and depended mostly on NO x -(mainly nitrate) from the overlying water. The NO x -consumption rates were correlated with NO x -concentration in the overlying water, but the sediment was a sink for NO x -only at the highest NO x -concentrations. The effect of changing light conditions on NO x -consumption was significant at the station with the highest NO x -level but not at other stations. KEY WORDS: Nitrate biosensor · Nitrogen transformation Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 26: [181][182][183][184][185][186][187][188][189][190][191][192][193] 2001 Studies using Liquid Ion eXchange-type (LIX) nitrate microsensors have provided detailed information about the distribution of nitrate in lake sediments and how this is affected by changes in light or oxygen conditions (De Beer & Sweerts 1989, Jensen et al. 1993, 1994. These studies, however, are restricted to freshwater systems because the LIX sensors are susceptible to interference from other ions such as bicarbonate and chloride (Jensen et al. 1993, Verschuren et al. 1999). The NO x -biosensor used in this study is highly sensitive, and the only interfering substance is nitrous oxide. It thus provides us with a new tool to investigate the microscale distribution of NO x -in marine environments.The aim of this study is to describe variations in the microscale distribution of NO x -and NO x -production/consumption at various light intensities along a gradient in water phase salinity and nitrate concentrations. We stress that the present study describes the net NO x -production/consumption rates. A number of biological processes such as denitrification, dissimilative reduction of NO x -to ammonia, oxidation of ammonia with nitrite (anammox), and NO x -assimilation may contribute to...

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Comparison of denitrification rate estimation techniques in a large, shallow lake

Cited by 58 publications

References 14 publications

Nitrogen mass balances and denitrification rates in central Ontario Lakes

Nitrogen mass balances and denitrification rates in central Ontario Lakes

Denitrification measurements in aquatic sediments: A comparison of three methods

Use of NOx- microsensors to estimate the activity of sediment nitrification and NOx- consumption along an estuarine salinity, nitrate, and light gradient

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