Comparison of cytotoxic extracts from fruiting bodies, infected insects and cultured mycelia of Cordyceps formosana

Lu, Ruili; Bao, Guan‐Hu; Hu, Fenglin; Huang, Bo; Li, Chunru; Li, Zengzhi

doi:10.1016/j.foodchem.2013.09.001

Cited by 12 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…were detected in the fruiting body of O. formosana (He et al 2007, 2010). Also, some reports on the comparison of cytotoxic extracts from fruiting bodies, infected insects and cultured mycelia of O. formosana revealed that rugulosin was cytotoxic against Chinese hamster ovary (CHO) cells, while skyrin was non-cytotoxic against CHO cells (He et al 2007, 2008; Lu et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Analysis of radical scavenging active components in the fermented mycelia of Ophiocordyceps formosana

Cheng

Da³

et al. 2017

Mycology

Self Cite

View full text Add to dashboard Cite

The rates of 1,1-diphenyl-2-picryhydrazyl (DPPH)-free radical scavenging and hydroxyl radical eliminating were employed as indexes to qualitatively and quantitatively analyse the radical scavenging activity of the extracts with different solvents from fermented mycelia of Hirsutella huangshanensis RCEF0868, the anamorph of Ophiocordyceps formosana (FMOF). The results showed that both the aqueous extract and the methanol extract had significant radical scavenging activity. The half maximal inhibitory concentrations (IC50) of the aqueous extract on DPPH-free radical and hydroxyl radical are 0.85 and 1.37 mg/mL, respectively, while the IC50 of methanol extract is 1.23 and 2.91 mg/mL, respectively. The combined liquid chromatography-diode array detector-high resolution mass spectrometer(HRMS) analysis and activity determination revealed that the molecular formulas of three radical scavengers in aqueous extract were C6H14O6(1), C14H17N5O8(2) and C20H33N5O9(3). In addition to the compound 1, the methanol extract had another three radical scavengers as C30H22O11(4), C30H22O10(5) and C30H18O10(6). All the six kinds of components exhibited the DPPH radical scavenging activity, and compounds 1, 4 and 5 also showed the hydroxyl radical eliminating activity at the same time. By querying the database of natural products and reference to the relevant reports, compound 3 may be a new entity. The others were respectively cordycepic acid, succinoadenosine, 4α-oxyrugulosin, rugulosin and skyrin and, among them, succinoadenosine and 4α-oxyrugulosin were found in entomogenous fungi for the first time.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of radical scavenging active components in the fermented mycelia of Ophiocordyceps formosana

Cheng

Da³

et al. 2017

Mycology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, the artificial mycelial biomass shows a close chemical composition [5,6] and has a similar pharmacological efficacy to natural products of Cordyceps sp. [7,8]. So, the cultivated mycelia of Cordyceps fungi have become a main substitute of the natural species for commercial health food or drug formulation.…”

Section: Introductionmentioning

confidence: 99%

Composition and Antitumor Activity of the Mycelia and Fruiting Bodies of Cordyceps militaris

Liu¹,

Huang²,

Zhou³

2014

JFNR

View full text Add to dashboard Cite

Cordyceps militaris contains many kinds of bioactive components with the various physiological activities, but has less natural sources of fruiting bodies. Compared with the fruiting bodies from the same origin, the fermented mycelia contained significantly higher amount of protein, crude fat and polysaccharides. The mycelia had more amount of the total amino acids, with proline (3.84 ± 0.12 g 100g -1 ), leucine (2.96 ± 0.10 g 100g -1 ), and aspartic acid (2.68 ± 0.06 g 100g -1 ) as the top three aboundant amino acids, while glutamic acid (3.56 ± 0.07 g 100g - 1), aspartic acid (2.52 ± 0.13 g 100g -1 ), and valine (2.35 ± 0.11 g 100g -1 ) in fruiting bodies. The tested minerals in the mycelia were much more than those in fruiting bodies, especially Selenium which was nondetectable in fruiting bodies. However, the mycelia had lower level of cordycepin and mannitol. The mycelia also exhibited higher antitumor activity against six tested human cancer cell lines, with the range of IC 50 (μg ml -1 ) from 25.03 ± 1.37 to 39.81 ± 0.54. In the animal test for liver cancer cell Hep G2, even high-dose treatment (250 mg kg-1 BW/day) of the extract of the mycelia inhibited the growth of tumor by only 27.6%, significantly lower than the positive control, CTX (30 mg kg -1 BW/day), but had no influence on body weight. The results confirm the potential of cultivated mycelia to be a good substitute of natural products.

show abstract

“…The possibly devastating effects of collection on biodiversity is also an important and controversial concern . Thus, mycelial culture has been suggested as an alternative solution for biological and chemical studies of macro-fungi, although the chemical profiles of fruiting bodies and mycelial cultures are often different. , …”

mentioning

confidence: 99%

“…3 Thus, mycelial culture has been suggested as an alternative solution for biological and chemical studies of macro-fungi, although the chemical profiles of fruiting bodies and mycelial cultures are often different. 4,5 As structure-based discovery has risen as an alternative approach for natural product discovery, 6 collections of source organisms are becoming increasingly important. In structurebased workflows, the targeting of organisms for isolation is prioritized based on data related to their metabolic phenotypes and potential, which are typically provided by untargeted metabolomics or genome mining efforts.…”

mentioning

confidence: 99%

Species Prioritization Based on Spectral Dissimilarity: A Case Study of Polyporoid Fungal Species

Jeong

Woo

et al. 2021

J. Nat. Prod.

View full text Add to dashboard Cite

Biological species collections are critical for natural product drug discovery programs. However, prioritization of target species in massive collections remains difficult. Here, we introduce an untargeted metabolomics-based prioritization workflow that uses MS/MS molecular networking to estimate scaffold-level distribution. As a demonstration, we applied the workflow to 40 polyporoid fungal species. Nine species were prioritized as candidates based on the chemical structural and compositional similarity (CSCS) metric. Most of the selected species showed relatively higher richness and uniqueness of metabolites than those of the others. Cryptoporus volvatus, one of the prioritized species, was investigated further. The chemical profiles of the extracts of C. volvatus culture and fruiting bodies were compared, and it was shown that derivative-level diversity was higher in the fruiting bodies; meanwhile, scaffold-level diversity was similar. This showed that the compounds found from a cultured fungus can also be isolated in wild mushrooms. Targeted isolation of the fruiting body extract yielded three unknown (1−3) and six known (4−9) cryptoporic acid derivatives, which are drimane-type sesquiterpenes with isocitric acid moieties that have been reported in this species. Cryptoporic acid T (1) is a trimeric cryptoporic acid reported for the first time. Compounds 2 and 5 exhibited cytotoxicity against HCT-116 cell lines with IC 50 values of 4.3 and 3.6 μM, respectively.

show abstract

Comparison of cytotoxic extracts from fruiting bodies, infected insects and cultured mycelia of Cordyceps formosana

Cited by 12 publications

References 34 publications

Analysis of radical scavenging active components in the fermented mycelia of Ophiocordyceps formosana

Analysis of radical scavenging active components in the fermented mycelia of Ophiocordyceps formosana

Composition and Antitumor Activity of the Mycelia and Fruiting Bodies of <i>Cordyceps </i><i>m</i><i>ilitaris</i>

Species Prioritization Based on Spectral Dissimilarity: A Case Study of Polyporoid Fungal Species

Contact Info

Product

Resources

About