Comparison of Cytokine Expression in Mesenchymal Stem Cells from Human Placenta, Cord Blood, and Bone Marrow

Abstract

Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROα(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.

Impact of Mesenchymal Stem Cell secreted PAI-1 on colon cancer cell migration and proliferation

“…1A) As previously reported in a number of studies, MSCs secreted a number of factors including Interleukin-6, MIF and PAI-1 [18]. HT29 and HCT-116 ( Conversely, MSCs were found to secrete highest levels of PAI-1 (10.6 ng/mL, 3.68-14.65 ng/mL).…”

“…Since MSCs are known to be present in colonic tumours and are known to produce pro-inflammatory cytokines, including PAI-1 and MIF [18], this study aimed to investigate cytokine secretion and functional effects of MSCs on colon cancer cells.…”

Mitochondrial Function and Energy Metabolism in Umbilical Cord Blood- and Bone Marrow-Derived Mesenchymal Stem Cells

“…Previous comparative studies on proliferation rate, gene expression profile, differentiation potential, and efficiency of UCBMSC, fetal BMMSC, adult BMMSC, and adiposederived hMSCs have produced conflicting results, although all hMSC types have been demonstrated to show systematically similar cell surface antigen expression [16][17][18][19][20][21]. In this study, we focused on comparison of UCBMSCs and BMMSCs from different age groups.…”

“…Several comparative studies on differentiation potency, proliferation rate, immunophenotype, and gene expression of hMSCs from different sources have been previously conducted [16][17][18][19][20][21]. In general, cell surface antigen characteristics are similar for hMSCs derived from umbilical cord, fetal bone marrow, adult bone marrow, and adipose tissue; but inconsistencies in differentiation potential and proliferation rate have been reported.…”

Secretion of immunoregulatory cytokines by mesenchymal stem cells

“…Limited data demonstrate that some but not all MSCs are a source of sICAM. Profiles of cytokine arrays revealed high expression of sICAM from human MSCs derived from umbilical cord and deciduas [221,222] and null expression from bone marrow-derived MSCs [221] . The exact physiological role of sICAM in health and pathology is still not completely revealed but reports demonstrate its potential to stimulate endothelial cell differentiation in conditions with angiogenic growth in tumorigenesis [223] .…”