Comparison of Culture With the Polymerase Chain Reaction for Detection of Gennital Mycoplasma

Peerayeh, Shahin Najar; Samimi, R

doi:10.29333/ejgm/82587

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…In this study, we compared culture with PCR for the detection of genital Mycoplasma. The results showed that PCR is a faster, more sensitive (93%), and easier method than culture, similar to previous studies (18,19).…”

Section: Discussionsupporting

confidence: 89%

“…The main technique for detection of Mycoplasma is culture, but the isolation of these microorganisms is difficult and requires a specific culture medium. PCR can detect many infectious diseases, particularly those caused by microorganisms that are difficult to cultivate (18). In this study, we compared culture with PCR for the detection of genital Mycoplasma.…”

Section: Discussionmentioning

confidence: 99%

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Investigating Chlamydia trachomatis and Genital Mycoplasma Prevalence and Apoptosis Markers in Infertile and Fertile Couples

Moosavian

Ghadiri

Amirzadeh³

et al. 2019

Jundishapur J Microbiol

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Background: Chlamydia trachomatis, Ureaplasma urealyticum, and Mycoplasma hominis are common sexually transmitted microorganisms. Objectives: The aim of the study was to determine the prevalence of these microorganisms in infertile couples and the effect of these infections on semen parameters. Methods: In this case-control study, samples were collected from 50 infertile couples and 50 fertile women and men. Specimens were examined for the presence of C. trachomatis, M. hominis, and U. urealyticum by culture and PCR. Semen specimens were analyzed for apoptotic markers using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and Western blot. Results: In this study, out of 50 semen specimens from infertile men, U. urealyticum and M. hominis were detected in 14 (28%) and 11 (22%) specimens by PCR, and five (10%) and one (2%) specimens by culture, respectively. C. trachomatis was found in five (10%) samples by PCR. In addition, out of 50 endocervical swabs from infertile women, C. trachomatis was found in seven (14%) specimens by PCR, U. urealyticum and M. hominis were detected in 25 (50%) and four (8%) swabs by PCR, and 13 (26%) and two (4%) by culture, respectively. The Western blot and TUNEL assay demonstrated a significant increase in caspase-3 activation and DNA fragmentation in the semen samples of the infected infertile men compared to uninfected men (6 vs. 1.5; P < 0.05). Conclusions: The results demonstrated that C. trachomatis and genital Mycoplasma are widespread among infertile couples, and these infections may lead to decreased semen quality.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Investigating Chlamydia trachomatis and Genital Mycoplasma Prevalence and Apoptosis Markers in Infertile and Fertile Couples

Moosavian

Ghadiri

Amirzadeh³

et al. 2019

Jundishapur J Microbiol

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Mycoplasma hominis profile in women: Culture, kit, molecular diagnosis, antimicrobial resistance, and treatment

ÖZTÜRK

Yıldız

Dursun

et al. 2019

Microbial Pathogenesis

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Survey on association between Mycoplasma hominis endocervical infection and spontaneous abortion using Polymerase Chain Reaction

Farhadifar

Khodabandehloo

Ramazanzadeh

et al. 2016

IJRM

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Background:Mycoplasma infections are suggested as etiology of adverse pregnancy outcomes. Objective:The aim of this study was to evaluate the association of Mycoplasma hominis (M. hominis) infection and spontaneous abortion among pregnant women.Materials and Methods:In this case-control study that was conducted from August 2012 to January 2013, totally, 109 women were included with spontaneous abortion with gestational ages of 10-20 weeks (Cases), and 109 women with normal pregnancy with gestational ages between 20-37 weeks (Controls) in Sanandaj, Iran. Using specific primers and extracted DNA from endocervical swabs, a PCR test was conducted for detection of M. hominis infection in women. For comparison of qualitative and quantitative variables, independent Fisher tests were used and p<0.05 was considered significant.Results:The total frequency of M. hominis infection was 6 (2.75%) in women. The frequency of M. hominis infection was 2 (1.83%) in the case group (spontaneous abortion) and 4 (3.66%) in the control group, respectively. In both case and control groups, no association was seen between M.hominis infection and spontaneous abortion (OR=0. 49, CI 95%: 0.08-2.73, p=0. 683). Conclusion:M. hominis was positive in the genital tract of some pregnant women, but it was not associated with spontaneous abortion. However, to prevent adverse pregnancy outcomes in women, foetus and neonate, routine screening and treatment for the genital Mycoplasma is recommended.

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Comparison of Culture With the Polymerase Chain Reaction for Detection of Gennital Mycoplasma

Cited by 6 publications

References 22 publications

Investigating Chlamydia trachomatis and Genital Mycoplasma Prevalence and Apoptosis Markers in Infertile and Fertile Couples

Investigating Chlamydia trachomatis and Genital Mycoplasma Prevalence and Apoptosis Markers in Infertile and Fertile Couples

Mycoplasma hominis profile in women: Culture, kit, molecular diagnosis, antimicrobial resistance, and treatment

Survey on association between Mycoplasma hominis endocervical infection and spontaneous abortion using Polymerase Chain Reaction

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