Comparison of Culture Results between Specimens from Corneal Scraping with Microhomogenization and Corneal Swab in Moderate and Severe Bacterial Corneal Ulcers

Martha, Faraby; Edwar, Lukman; Karuniawati, Anis; Fuady, Ahmad; Tuasikal, Ramadhiana Maktazula

doi:10.1128/spectrum.03565-22

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…11 scalpel blade, and a thin smear of tissue was placed on two glass slides for microbiological examination. Additional scrapings were then taken and applied to culture media, including blood agar, chocolate agar, and Sabouraud dextrose agar [ 19 , 20 ] .…”

Section: Methodsmentioning

confidence: 99%

Frequency and visual outcomes of herpes simplex keratitis in a Kuwaiti tertiary referral center

Lotfy,

Aldhefeery,

Alenezi

et al. 2023

Med Hypothesis Discov Innov Ophthalmol

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Background: Herpes simplex virus (HSV), a cause of many ocular pathologies, may affect the corneal epithelium, stroma, or endothelium, with the epithelium as the most frequently affected location. We aimed to determine the frequency and visual outcomes of HSV keratitis over a 1-year period at Farwaniya Hospital, Kuwait. Methods: This prospective observational case series included patients diagnosed with HSV keratitis who presented to the outpatient or eye casualty department between September 2022 and September 2023. Full history, demographic profile, detailed ocular and slit-lamp examinations, and visual acuity assessments were recorded prior to and after treatment. Baseline corneal sensation was assessed in the four corneal quadrants using the cotton wisp test. Results: Of the 227 patients who presented with infectious keratitis, 72 (31.7%) were diagnosed with HSV keratitis. The mean (SD) age was 45.86 (16.06) years, and 48 (66.7%) patients were male. The subtypes of HSV keratitis were endotheliitis in 27 (37.5%) patients, dendritic ulcer in 22 (30.6%), stromal keratitis with ulceration in 9 (12.5%) and without ulceration in 8 (11.1%), and geographic ulcer in 6 (8.3%). The mean baseline best-corrected distance visual acuity (BCDVA) differed significantly between the HSV keratitis subtypes (P < 0.001). Most patients (n = 70, 97.2%) had unilateral eye involvement, were treated within 1 – 2 weeks, and demonstrated improved BCDVA. Despite the improvement in BCDVA in all HSV keratitis subtypes, a significant difference in the median BCDVA remained after treatment (P < 0.001). Pairwise comparisons revealed a significantly better BCDVA after treatment in eyes with dendritic epithelial ulcers than in eyes with HSV stromal keratitis with ulceration (P = 0.003) or geographic epithelial ulcers (P = 0.005). After treatment, corneal haze and neovascularization were detected in 54 (75.0%) and 24 (33.3%) patients, respectively. Conclusions: We detected a substantial frequency of HSV keratitis in one of the governorates of Kuwait, with endotheliitis as the most common manifestation. Timely standard treatment interventions based on valid guidelines resulted in BCDVA improvement in all subtypes of HSV keratitis in our series, indicating the importance of early treatment. However, the BCDVA in eyes with dendritic epithelial ulcers remained substantially better than that in eyes with geographic epithelial ulcers or HSV stromal keratitis with ulceration, signifying the effect of the initial HSV keratitis subtype on visual outcomes. To our knowledge, this is the first study to highlight the frequency of HSV keratitis in a Kuwaiti hospital, and larger-scale research in this region is needed to further understand and manage the condition.

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Section: Methodsmentioning

confidence: 99%

Frequency and visual outcomes of herpes simplex keratitis in a Kuwaiti tertiary referral center

Lotfy,

Aldhefeery,

Alenezi

et al. 2023

Med Hypothesis Discov Innov Ophthalmol

View full text Add to dashboard Cite

show abstract

Comparison of Culture Results between Specimens from Corneal Scraping with Microhomogenization and Corneal Swab in Moderate and Severe Bacterial Corneal Ulcers

Abstract: This study aimed to compare the culture results between scraping specimens with microhomogenization and corneal smears in patients with moderate to severe bacterial corneal ulcers. This study could be a guideline for treating bacterial corneal ulcers.

Cited by 1 publication

References 15 publications

Frequency and visual outcomes of herpes simplex keratitis in a Kuwaiti tertiary referral center

Frequency and visual outcomes of herpes simplex keratitis in a Kuwaiti tertiary referral center

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