Comparison of Culture Media for <i>in vitro</i> Maturation of Oocytes of Indigenous Zebu Cows in Bangladesh

Singha, Joydev Kumar; Bhuiyan, Mohammad Musharraf Uddin; Rahman, Mohammad Moshiur; Bari, Farida Yeasmin

doi:10.12750/jet.2015.30.4.327

Cited by 2 publications

(4 citation statements)

References 16 publications

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“…A total of 2340 oocytes were collected from 585 ovaries and the mean number of oocytes collection from each ovary was 4.00. The present mean number of retrieved oocytes per ovary is higher than that of previous study (Morshed et al, 2014;Singha et al, 2015;Choudhury et al, 2017) and lower than that of an earlier study (Talukder et al, 2008). The reason for variations in oocyte retrieval rate among studies may be due to variation in skillness of follicle aspirators.…”

Section: Resultscontrasting

confidence: 80%

“…Further, similar result to earlier report has been demonstrated elsewhere (Schellander et al, 1990). There are different sources for supplemented sera such as FBS (Kobayeshi et al, 1994;Nandi, 1998;Mahmoud and Nawito, 2005;Das et al, 2006;Talukder et al, 2008), OCS (Schellander et al, 1990;Singha et al, 2015), steer serum (Roy et al, 1968;Nandi et al, 2001) and superovulated cow serum (Boediono et al, 1994). Although, there are reports to use different sera sources for supplementation in maturation medium, comparison was made within FBS, OCS and BSA only in the present study.…”

Section: Experiments 3 Determination Of An Effective Protein Supplemementioning

confidence: 87%

“…In vitro maturation (IVM) of oocytes is the first and most important step for any IVEP-ET programme in cattle. However, although several preliminary studies have been conducted on IVM of oocytes of zebu cows in Bangladesh (Das et al, 2006;Islam et al, 2007;Talukder et al, 2008;Morshed et al, 2014;Singha et al, 2015;Choudhury et al, 2017) still the IVM rate needs to be improved. The maturation medium and supplementation of protein and hormones in it may play an important role for IVM rate and subsequent development after in vitro fertilization (IVF) (Bavister et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Determination of an Effective Media and Its Hormone and Protein Supplemenntation for in Vitro Maturation of Oocytes of Indigenous Zebu Cows

Rahman¹,

Morshed²,

Juyeana³

et al. 2018

Bangl. J. Vet. Med.

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In vitro maturation (IVM) of oocytes is the first important step for successful in vitro embryo production of any mammalian species. The objectives of the present study were to determine an effective basic medium and its hormone and protein supplementation for IVM of oocytes of indigenous zebu cows. The oocytes were derived from ovaries of locally slaughtered cows after aspiration of follicle. The oocytes were cultured in medium for 24 hrs at 38.5ºC with 5% CO2 in humidified air for maturation. The maturation of oocytes was evaluated by examining the presence of first polar body extrusion in denuded oocytes under inverted microscope. To determine an effective basic medium, the oocytes were cultured in fetal bovine serum (FBS) supplemented tissue culture medium (TCM), modified synthetic oviduct fluid (mSOF) and Tyrodes albumin lactate pyruvate (TALP) medium. The maturation rate was significantly higher (74±4.2) in TCM medium than that of TALP medium (58.2±6.2). To determine an effective hormone supplementation for maturation medium, the oocytes were cultured in either in follicle stimulating hormone (FSH) or gonadotrophin supplemented TCM. The maturation rate of oocytes was significantly (p>0.05) higher (73.3±4.0) in FSH supplemented medium than that of gonadotrophin supplemented counterpart (60.2±6.6). To determine an effective protein supplementation, the oocytes were cultured in FBS, oestrus cow serum (OCS) and bovine serum albumin (BSA) supplemented TCM 199. The maturation rate of oocytes were 73.0±5.9, 71.1±2.8, and 62.5±9.4 in medium supplemented with FBS, OCS and BSA respectively (p>0.05). In conclusions, TCM supplemented with either FBS, OCS or BSA as protein and FSH as hormone may be used as medium for IVM of oocytes of indigenous zebu cows.

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Section: Resultscontrasting

confidence: 80%

Section: Experiments 3 Determination Of An Effective Protein Supplemementioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Determination of an Effective Media and Its Hormone and Protein Supplemenntation for in Vitro Maturation of Oocytes of Indigenous Zebu Cows

Rahman¹,

Morshed²,

Juyeana³

et al. 2018

Bangl. J. Vet. Med.

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“…Other studies reported that oocytes obtained in medium supplemented with growth hormone (GH), IGF-I, EGF, thyroxin (T4), and FSH had the maximum percentage of M-II stage oocytes (38.6%) when compared to the other treatments, revealing that in vitro development of goat PFs could be significantly improved through addition of GH, EGF, T4, and FSH in the best combination [52]. However, differential rates of maturation of cattle oocytes in vitro were found upon supplementation of FBS (81.7% ± 12.9%) and estrous calf serum (85.7 ± 12.7%) in TCM-199 containing 5 µg/mL FSH and 1 µg/mL estradiol [53].…”

Section: Ivm In 25 Ng/ml Leptin With Different Combinations Of Igf-i Egf Fbs and Bsamentioning

confidence: 96%

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Talukdar¹,

Biswas²,

Kadirvel³

et al. 2020

Turk J Vet Anim Sci

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The present study aimed to evaluate the effect of supplementing epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), fetal bovine serum (FBS), and bovine serum albumin (BSA) in different combinations in a maturation medium (MM) comprising TCM-199 + 5 µg/mL 17-β estradiol + 5 µg/mL ovine-luteinizing hormone (oLH) + 5 µg/mL pure follicle-stimulating hormone (pFSH) + 100 µM/mL cysteamine containing the best concentration of leptin among 10/20/25/30 ng/mL on in vitro maturation (IVM) and subsequent vitrification of matured goat oocytes. The IVM of oocytes was carried out in a CO 2 incubator for 27 h at 38.5 °C. The percentage of oocytes with cumulus expansion and polar body extrusion was found to be highest in 25 ng/mL leptin at 47.37% and 15.63%; rates of 66.67% and 28.57% were obtained when supplemented with 50 ng/mL IGF-I + 10 ng/mL EGF in medium containing 25 ng/mL leptin as compared to the other media. The in vitro matured oocytes were vitrified in 5 M ethylene glycol + 5 M propylene glycol with 0.5 M sucrose in TCM-199 + 20% FBS using liquid nitrogen. The highest percentage of morphologically intact in vitro matured oocytes following vitrification was obtained in medium containing 25 ng/mL leptin (89.29%). It was concluded that addition of IGF-I + EGF with 25 ng/mL leptin in MM yielded a higher rate of maturation of oocytes, and supplementation of 25 ng/mL leptin in MM resulted in a higher rate of morphologically intact vitrified oocytes, indicating the efficiency of leptin for cryotolerance.

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Comparison of Culture Media for in vitro Maturation of Oocytes of Indigenous Zebu Cows in Bangladesh

Cited by 2 publications

References 16 publications

Determination of an Effective Media and Its Hormone and Protein Supplemenntation for in Vitro Maturation of Oocytes of Indigenous Zebu Cows

Determination of an Effective Media and Its Hormone and Protein Supplemenntation for in Vitro Maturation of Oocytes of Indigenous Zebu Cows

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

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