Comparison of cryopreserved human sperm from solid surface vitrification and standard vapor freezing method: on motility, morphology, vitality and DNA integrity

Satirapod, Chonthicha; Treetampinich, Chatchai; Weerakiet, Sawaek; Wongkularb, A; Rattanasiri, Sasivimol; Choktanasiri, Wicharn

doi:10.1111/j.1439-0272.2011.01267.x

Cited by 35 publications

(30 citation statements)

References 25 publications

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“…In this regard, the best results reported in the literature stem from experiments with human sperm (Satirapod et al. ), inclusive studies when no CPAs were added (Saki et al. ), while some attempts have recently been carried out in animals (Sánchez et al.…”

Section: Discussionmentioning

confidence: 99%

Impact of Ultra‐Rapid Freezing on the Motility, Morphology, Viability and Acrosome Integrity of Epididymal Cat Sperm Diluted in Sucrose‐Based Extenders

Vizuete

Jiménez

Agüera

et al. 2013

Reprod Domestic Animals

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The objective of the present study was to investigate the influence of different sucrose-based extenders on the motility, morphology, viability and acrosomal integrity of epididymal cat spermatozoa cryopreserved by ultra-rapid freezing method. Nine cats were castrated, and collected semen was diluted 1 : 1 with Dulbecco`s phosphate-buffered saline-BSA1%-based extender supplemented with different sucrose concentrations (0, 0.25, 0.4 and 0.6 m). After ultra-rapid freezing, samples were thawed and sperm motility, morphology, viability and acrosome status were assessed. At thawing, the number of progressively motile (p < 0.01) and morphologically normal (p < 0.01) sperm was higher in the sucrose-supplemented groups than in the sucrose-free group. Viability of spermatozoa cryopreserved without sucrose was significantly reduced. In extender supplemented with 0.4 m sucrose, spermatozoa viability showed higher values (57.0 ± 4.7; p < 0.01). No significant differences were detected among groups for sperm acrosome integrity. Results support that cat sperm survive after ultra-rapid freezing using sucrose as a cryoprotectant, and the best results were achieved when 0.4 m of sucrose was used. This is the first report on sperm ultra-rapid freezing of cat sperm and further studies on extenders, sperm management or cryovials should be carried out to improve sperm cryosurvival.

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Section: Discussionmentioning

confidence: 99%

Impact of Ultra‐Rapid Freezing on the Motility, Morphology, Viability and Acrosome Integrity of Epididymal Cat Sperm Diluted in Sucrose‐Based Extenders

Vizuete

Jiménez

Agüera

et al. 2013

Reprod Domestic Animals

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show abstract

“…With a view to improving human sperm recovery and avoiding cryodamage, various methods of sperm cryopreservation have been developed and compared in terms of sperm viability and motility (for a review, see Royere et al, 1996; Oehninger et al, 2000; Di Santo et al, 2012). In 2011, no fewer than 7 publications presented and compared new cryopreservation methods (Creemers et al, 2011; Isachenko et al, 2011; Martinez‐Soto et al, 2011; Menegazzo et al, 2011; Peng et al, 2011; Rahana et al, 2011; Satirapod et al, 2012). Recently, a model of sperm cryodamage was developed (John Morris et al, 2012) in order to try to understand the effects of slow and rapid freezing or vitrification on spermatozoa cells.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Human Spermatozoa Decreases the Number of Motile Normal Spermatozoa, Induces Nuclear Vacuolization and Chromatin Decondensation

Boitrelle¹,

Albert²,

Theillac³

et al. 2012

Journal of Andrology

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Even though cryopreservation of human spermatozoa is known to alter sperm motility and viability, it may also induce nuclear damages. The present study set out to determine whether or not cryopreservation alters motile sperm morphology under high magnification and/or is associated with chromatin decondensation. For 25 infertile men, we used high-magnification microscopy to determine the proportions of various types of motile spermatozoa before and after freezing-thawing: morphometrically normal spermatozoa with no vacuole (grade I), #2 small vacuoles (grade II), at least 1 large vacuole or .2 small vacuoles (grade III), and morphometrically abnormal spermatozoa (grade IV). The spermatozoa's chromatin condensation and viability were also assessed before and after freezing-thawing. Cryopreservation induced sperm nuclear vacuolization. It decreased the proportion of grade I + II spermatozoa (P , .001). It induced a decrease in the sperm viability rate (P , .001) and increased the proportion of sperm with noncondensed chromatin (P , .001). The latter parameter was strongly correlated with sperm viability (r 5 0.71; P , .001). However, even motile sperm presented a failure of chromatin condensation after freezingthawing, because the proportion of sperm with noncondensed chromatin was correlated with high-magnification morphology (r 5 20.49 and 0.49 for the proportions of grade I + II and grades III + IV, respectively; P , .001). Cryopreservation alters the organelle morphology of motile human spermatozoa and induces sperm chromatin decondensation. High-magnification microscopy may be useful for evaluating frozen-thawed spermatozoa before use in assisted reproductive technology procedures (such as intrauterine insemination, in vitro fertilization, and intracytoplasmic sperm injection) and for performing research on cryopreservation methods. If frozen-thawed sperm is to be used for intracytoplasmic sperm injection, morphological selection under high magnification may be of particular value.

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“…Because of that, it is important to understand the effects of cryopreservation to preserve the better quality of the thawed sample. It has been shown that cryopreservation reduces sperm motility and sperm vitality (Thomson et al ., ; Lee et al ., ; Satirapod et al ., ). Recent studies have been focused on the effect of cryopreservation on sperm DNA damage, showing that the main effector of DNA damage during the process of freezing and thawing a semen sample are the reactive oxygen species (Lasso et al ., ; Thomson et al ., ; Said et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Human semen cryopreservation: a sperm DNA fragmentation study with alkaline and neutral Comet assay

et al. 2013

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SUMMARYSperm cryopreservation is widely used for both research and reproduction purposes, but its effect on sperm DNA damage remains controversial. Sperm DNA fragmentation (SDF) has become an important biomarker to assess male infertility. In particular, the differentiation between single-and double-stranded DNA fragmentation (ssSDF and dsSDF) has clinical implications for male infertility where ssSDF is associated with reduced fertility, whereas dsSDF is associated with increased risk of miscarriage. In this study, semen samples from 30 human males have been analysed in both fresh and cryopreserved using the alkaline and neutral Comet assays. Results show an increase of about 10% of ssSDF, assessed by the alkaline Comet assay, regardless of the male fertility status. Neutral Comet analysis of dsSDF does not show any statistical increase when comparing fresh and cryopreserved samples in any of the patient groups. Results support previous reports that oxidative stress is the major effector in DNA damage during sample cryopreservation, as, on one hand, ssSDF has previously been related to oxidative damage and, on the other hand, we have not found any effect on dsSDF. Therefore, there might be a slight risk of decreased fertility after using a freezed sample, but no evidence for increased miscarriage risk from cryopreserved spermatozoa should be expected.

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Comparison of cryopreserved human sperm from solid surface vitrification and standard vapor freezing method: on motility, morphology, vitality and DNA integrity

Cited by 35 publications

References 25 publications

Impact of Ultra‐Rapid Freezing on the Motility, Morphology, Viability and Acrosome Integrity of Epididymal Cat Sperm Diluted in Sucrose‐Based Extenders

Impact of Ultra‐Rapid Freezing on the Motility, Morphology, Viability and Acrosome Integrity of Epididymal Cat Sperm Diluted in Sucrose‐Based Extenders

Cryopreservation of Human Spermatozoa Decreases the Number of Motile Normal Spermatozoa, Induces Nuclear Vacuolization and Chromatin Decondensation

Human semen cryopreservation: a sperm DNA fragmentation study with alkaline and neutral Comet assay

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