Comparison of convolution and pulse methods for line narrowing in protein NMR spectra

Gassner, M.; Jardetzky, Oleg; Conover, Woodrow W.

doi:10.1016/0022-2364(78)90234-2

Cited by 18 publications

(9 citation statements)

References 7 publications

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“…Since homonuclear decoupling was used to suppress the residual HDO line in this spectrum, the intensities are likely to be effected by cross-relaxation phenomena and thus unfortunately cannot be used for direct estimation of the amino acid composition. Multiplication of the free induction decays with a sinebell results in line narrowing for sharp resonances and removes broad resonances from the spectrum (Gassner et al, 1978). When this technique was applied to phosvitin, all resonances sharpened in the NMR spectrum.…”

Section: Resultsmentioning

confidence: 99%

Structure of hen phosvitin: a phosphorus-31 NMR, proton NMR, and laser photochemically induced dynamic nuclear polarization proton NMR study

Vogel¹

1983

Biochemistry

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Section: Resultsmentioning

confidence: 99%

Structure of hen phosvitin: a phosphorus-31 NMR, proton NMR, and laser photochemically induced dynamic nuclear polarization proton NMR study

Vogel¹

1983

Biochemistry

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“…For example, for Opa60, spectra from both spectrometers were sampled in the proton dimension to 9.1 ms for linewidth determination, and for nitrogen linewidth analysis, 12.5 ms nitrogen sampling was used at 1200 MHz and 15.75 ms for the 950 MHz spectra, ensuring a valid comparison in ppm, and >3 times T 2 for the narrowest signal identified. The 2D spectra were apodized with a cosine squared function (VDAC) or an 18 degree shifted cosine squared function (M2, Opa60, CitA) using the Qsine [ 63 , 64 ] function: sin [(π − π/SSB)t + π/SSB)] with SSB of 2 or 2.5 in order to minimally change the line shapes in 2D.…”

Section: Methodsmentioning

confidence: 99%

Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning

et al. 2021

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The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.

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“…The operation is a filtering function that weights later times in the FID [digital points containing information on resonances of narrower linewidths (longer TJ more heavily than earlier ones that contain more information about intrinsically broad components (short TJ]. This operation will result in distortion of relative intensities depending on differences in linewidths and degree of overlap with other resonances (Gassner et al, 1978). Unless large changes occur in individual resonance linewidths or in the degree of resonance overlap during an experiment, relative changes of a given resonance amplitude should not be significantly affected by this form of data processing.…”

Section: Materials and Methods Nmr Parameters For In Vivo Spectroscopymentioning

confidence: 99%

Effect of Hypoglycemic Encephalopathy upon Amino Acids, High‐Energy Phosphates, and pH_i in the Rat Brain In Vivo: Detection by Sequential ¹H and ³¹P NMR Spectroscopy

Behar¹,

Hollander²,

Petroff³

et al. 1985

Journal of Neurochemistry

156

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Metabolic alterations in amino acids, high-energy phosphates, and intracellular pH during and after insulin hypoglycemia in the rat brain was studied in vivo by 1H and 31P nuclear magnetic resonance (NMR) spectroscopy. Sequential accumulations of 1H and 31P spectra were obtained from a double-tuned surface coil positioned over the exposed skull of a rat while the electroencephalogram was recorded continuously. The transition to EEG silence was accompanied by rapid declines in phosphocreatine, nucleoside triphosphate, and an increase in inorganic orthophosphate in 31P spectra. In 1H spectra acquired during the same time interval, the resonances of glutamate and glutamine decreased in intensity while a progressive increase in aspartate was observed. Following glucose administration, glutamate and aspartate returned to control levels (recovery half-time, 8 min); recovery of glutamine was incomplete. An increase in lactate was detected in the 1H spectrum during recovery but it was not associated with any change in the intracellular pH as assessed in the corresponding 31P spectrum. Phosphocreatine returned to control levels following glucose administration, in contrast to nucleoside triphosphate and inorganic orthophosphate which recovered to only 80% and 200% of their control levels, respectively. These results show that the changes in cerebral amino acids and high-energy phosphates detected by alternating the collection of 1H and 31P spectra allow for a detailed assessment of the metabolic response of the hypoglycemic brain in vivo.

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Comparison of convolution and pulse methods for line narrowing in protein NMR spectra

Cited by 18 publications

References 7 publications

Structure of hen phosvitin: a phosphorus-31 NMR, proton NMR, and laser photochemically induced dynamic nuclear polarization proton NMR study

Structure of hen phosvitin: a phosphorus-31 NMR, proton NMR, and laser photochemically induced dynamic nuclear polarization proton NMR study

Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning

Effect of Hypoglycemic Encephalopathy upon Amino Acids, High‐Energy Phosphates, and pH_i in the Rat Brain In Vivo: Detection by Sequential ¹H and ³¹P NMR Spectroscopy

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Comparison of convolution and pulse methods for line narrowing in protein NMR spectra

Cited by 18 publications

References 7 publications

Structure of hen phosvitin: a phosphorus-31 NMR, proton NMR, and laser photochemically induced dynamic nuclear polarization proton NMR study

Structure of hen phosvitin: a phosphorus-31 NMR, proton NMR, and laser photochemically induced dynamic nuclear polarization proton NMR study

Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning

Effect of Hypoglycemic Encephalopathy upon Amino Acids, High‐Energy Phosphates, and pHi in the Rat Brain In Vivo: Detection by Sequential 1H and 31P NMR Spectroscopy

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Effect of Hypoglycemic Encephalopathy upon Amino Acids, High‐Energy Phosphates, and pH_i in the Rat Brain In Vivo: Detection by Sequential ¹H and ³¹P NMR Spectroscopy