Comparison of conventional Papanicolaou smears and a fluid-based, thin-layer system for cervical cancer screening

Lee, Kenneth R.; Ashfaq, Raheela; Birdsong, George G.; Corkill, Mary; McIntosh, Karen M.; Inhorn, Stanley L.

doi:10.1016/s0029-7844(97)00228-7

Cited by 248 publications

(116 citation statements)

References 14 publications

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“…It is very well known that up to 90% of the Pap material collected by health providers remains unused by pathologists for diagnosis [26,27,28,29,30]. In fact, one of the attractive points for switching from conventional to liquid-based technology was the improved ability of capturing more material for a more accurate diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Whole Slide Imaging of Pap Cell Block Preparations versus Liquid-Based Thin-Layer Cervical Cytology: A Comparative Study Evaluating the Detection of Organisms and Nonneoplastic Findings

Tawfik¹,

Davis²,

Dillon³

et al. 2014

Acta Cytologica

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Objective: Cervical cancer is one of the most common malignancies worldwide, yet it is preventable by population screening. In a previous study, we confirmed the feasibility of utilizing whole slide imaging (WSI) of cell block (CB) preparations to overcome the limitations of digitizing cytologic samples. In this study, we evaluated the accuracy of WSI in identifying various organisms and nonneoplastic findings. Study Design: A total of 335 WS images from Pap CB preparations were analyzed using the Aperio system. The test performance characteristics of ThinPrep (TP) and WSI samples were compared for adequacy, for the presence of bacterial vaginosis (BV), fungi, Trichomonas vaginalis (TV) and herpes simplex virus (HSV) and for nonneoplastic findings. Results: The WSI samples contained optimal material from all preparations. BV was diagnosed in 33 WSI versus 36 TP samples. Budding yeasts and/or pseudohyphal forms were noted in 18 WSI versus 19 TP samples. TV organisms (10 of 11 samples) and 1 HSV case were accurately identified in the WSI and TP samples. Squamous metaplasia, keratosis and reactive/reparative and inflammatory changes were easily identified by WSI. Conclusions: The concept of WSI from Pap CB preparations is potentially feasible for adoption. Digital remote web-based technology eliminates the need for an individual on site, saving time and resources.

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Section: Discussionmentioning

confidence: 99%

Whole Slide Imaging of Pap Cell Block Preparations versus Liquid-Based Thin-Layer Cervical Cytology: A Comparative Study Evaluating the Detection of Organisms and Nonneoplastic Findings

Tawfik¹,

Davis²,

Dillon³

et al. 2014

Acta Cytologica

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“…Cells processed with LBP seem to be over-stained compared with those processed conventionally. It is said [10 ]that the cells processed with SurePath [1,2] demonstrate a tendency towards shrinkage due to ethanol fixation, whereas those with ThinPrep do not [3,4,5]. The shrinkage of cells with the TACAS procedure may be in the range between these two methods.…”

Section: Discussionmentioning

confidence: 99%

“…Liquid-based cytological procedures [1,2,3,4,5,6] have become more widely used for cervical cancer screening, which may be due to the clear cytological specimens in the limited field of the glass slide that are produced with an automated smearing system. A new liquid-based procedure (LBP), the Thinlayer Advanced Cytology Assay System (TACAS™; MBL, Nagano, Japan), is now commercially available.…”

Section: Introductionmentioning

confidence: 99%

Application of a New Liquid-Based Procedure (TACAS) for the Screening of Cervical Cancer: A Preliminary Study

Kuramoto¹,

Iwami

Sugimoto

et al. 2012

Acta Cytologica

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Objective: To evaluate the usefulness of a new liquid-based cytological procedure in a population screening program for cervix cancer. Subjects and Methods: Subjects were 1,000 women who underwent primary screening at the Kanagawa Health Service Association. The cytological specimens obtained by either cotton stick and Cytobrush® or Cervex-Brush® were processed using the Thinlayer Advanced Cytology Assay System (TACAS™), following the manufacturer’s instructions. Results: (1) Cells were evenly distributed on specimens and stained evenly; (2) shrinkage of cells was 5% based on measurement of the nuclear diameters of granulocytes in comparison with those of the conventional procedure; (3) incidences of cells that occupied the whole area, 1/20≤, 1/4≤, 1/4> of the observation fields were 58.8, 26.2, 12.0 and 3.0%, respectively; (4) number of the squamous cells in cases with 1/4> was <5,000, in which specimen cells were correctly obtained from the squamocolumnar junction except in 3 cases (0.3%); (5) bleeding at cellular sampling was 5%, but did not disturb cell analysis; (6) inflammation caused by organisms was easily diagnosed; (7) detection rate of abnormal cytology was 4.3%, including ASC-US in 2.8% and ASC-H in 0.1%. Conclusion: TACAS is a feasible and useful cytological procedure.

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“…[13][14][15][16][17][18][19][20][21] In each trial both conventional and ThinPrep slides were prepared. This involved the clinician first making a conventional smear on a microscope slide and then rinsing the instruments containing the remaining cellular material into a vial containing PreservCyt solution to make a ThinPrep slide as outlined above.…”

Section: Introductionmentioning

confidence: 99%

“…ThinPrep was found to be superior to the conventional technique in the detection of low-grade epithelial abnormalities. 13,14,18,19 In a study comparing the ThinPrep technique with histology (gold standard), ThinPrep was shown to be significantly more sensitive than the conventional method for the detection of lowgrade squamous intraepithelial lesion (SIL) and more severe diseases. 22 The ThinPrep method showed no significant difference in specificity from the conventional method.…”

Section: Introductionmentioning

confidence: 99%

Removal of blood components from cervical smears: Implications for cancer diagnosis using FTIR spectroscopy

2002

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Red and white cell lysis buffers were investigated to determine their ability to remove blood components from cervical smears prior to IR microspectroscopy. A white cell lysis buffer was effective in increasing the spectral reproducibility and sample homogeneity and in reducing the presence of inflammatory exudate, particularly leukocytes, from cervical smears. The reduction of leukocytes appeared to cause abnormal samples to be grouped with normal samples, resulting in poor discrimination. Despite differences between the cytological and histological diagnoses of cervical abnormalities, the results indicate that the differences seen in the spectra of exfoliated malignant and nonmalignant cervical cells could be due to the presence of inflammation.

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Comparison of conventional Papanicolaou smears and a fluid-based, thin-layer system for cervical cancer screening

Abstract: The fluid-based, ThinPrep method of sample preparation resulted in a statistically significant increase in cytologic diagnosis of cervical cancer precursors and in specimen adequacy compared with the conventional Papanicolaou testing method.

Cited by 248 publications

References 14 publications

Whole Slide Imaging of Pap Cell Block Preparations versus Liquid-Based Thin-Layer Cervical Cytology: A Comparative Study Evaluating the Detection of Organisms and Nonneoplastic Findings

Whole Slide Imaging of Pap Cell Block Preparations versus Liquid-Based Thin-Layer Cervical Cytology: A Comparative Study Evaluating the Detection of Organisms and Nonneoplastic Findings

Application of a New Liquid-Based Procedure (TACAS) for the Screening of Cervical Cancer: A Preliminary Study

Removal of blood components from cervical smears: Implications for cancer diagnosis using FTIR spectroscopy

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