Comparison of conventional and liquid‐based cytology using The Paris System for Reporting Urinary Cytology

Goutas, Dimitrios; Savvidou, Kyriaki; Vrettou, K.; Meletis, Emmanouel; Levis, Panagiotis; Constantinides, C.; Lazaris, Andreas C.; Mikou, Panagiota

doi:10.1111/cyt.13040

Cited by 3 publications

(5 citation statements)

References 24 publications

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“…The overall accuracy was 79.8%. [ 7 ] Our values show similar percentages to their identified concordant and discordant cases as well as other analytical parameters, except from sensitivity and negative predictive value, which are significantly lower than what we calculated.…”

Section: Discussionsupporting

confidence: 84%

Cytospin performance when using Paris system for reporting urinary cytology

et al. 2022

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Objectives: The Paris System (TPS) for Reporting Urine Cytology has significantly improved the approach to evaluating urine cytology. TPS criteria were defined mainly according to ThinPrep and SurePath preparations, as they are widely utilized. The objective of this study is to validate urine cytology interpretation according to the TPS classification using cytospin technique in relation to the gold slandered histology. Material and Methods: This retrospective study examined and analyzed 316 urine specimens from King Abdulaziz University Hospital between 2015 and 2020. Cytospin technique is performed for all cases. Slides were recategorized using TPS criteria, then compared with the original histology diagnosis. Results: According to the TPS, 108 cases were classified as 101 AUC (32%), 95 NEG (30%), 59 HGUC (18.7%), 31 SHGUC (9.8%), and 30 (9.5%) others. The computed sensitivity of cytospin in urine cytology was 94.7%, with 73.9% specificity, a positive predictive value of 85.6%, a negative predictive value of 89.5%, and overall accuracy of 86.8%. Conclusion: Urine cytology testing is considered to be a non-invasive and sensitive method to screen for urothelial carcinoma. TPS defined standards are reliable on cytospin prepared slides for reporting urine cytology.

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Section: Discussionsupporting

confidence: 84%

Cytospin performance when using Paris system for reporting urinary cytology

et al. 2022

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“…When the recommended five HGCs by TPS is strictly applied, the ROHM is well above 90% 9 . Studies conducted by Goutas et al 20 . and Yamazaki et al 21 .…”

Section: Discussionmentioning

confidence: 94%

“…More so, the strict application of TPS understandably comes with a low‐to‐moderate sensitivity value (38%–56%) as reported in studies analyzing the impact of TPS, with specificity (97%–100%), PPV (93%–100%), and a NPV as high as 90% 21–24 . We used ThinPrep, and studies have shown that the use of the liquid‐based preparations is reliable, with high ROHM and high concordance among interpreters in detecting HGUC by application of TPS in lower urinary; the rate of detection of HGUC is not affected by the method of specimen collection (voided or collected by bladder washings) 10,20,25,26 …”

Section: Discussionmentioning

confidence: 98%

“…When the recommended five HGCs by TPS is strictly applied, the ROHM is well above 90%. 9 Studies conducted by Goutas et al 20 and Yamazaki et al 21 both reported ROHM of 95% and 97.8%, respectively. 20,21 In our study, taking into consideration all cases reviewed in this study including the three HGCs, our calculated ROHM was 98%.…”

Section: Gendermentioning

confidence: 96%

“…[21][22][23][24] We used ThinPrep, and studies have shown that the use of the liquidbased preparations is reliable, with high ROHM and high concordance among interpreters in detecting HGUC by application of TPS in lower urinary; the rate of detection of HGUC is not affected by the method of specimen collection (voided or collected by bladder washings). 10,20,25,26 Following review of the 36 cases of HGUC (from 22 patients) with negative histology (Cohort 2) on concurrent biopsy, we found all cases meeting the criteria for diagnosing HGUC in lower urinary tract specimens as outlined by TPS. None of these cases showed less than five HGCs.…”

Section: Gendermentioning

confidence: 99%

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Are we on track for diagnosing high‐grade urothelial carcinoma with a minimum quantity of five malignant cells in lower tract specimens? Critical analysis of The Paris System Quantitation Criteria

Folarin,

Siddiqui

2023

Cancer Cytopathology

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BackgroundThe Paris System for Reporting Urinary Cytology (TPS) has gained universal acceptance as the standard for reporting urine cytology requiring at least 5–10 malignant cells to diagnose high‐grade urothelial carcinoma (HGUC) in lower and upper urinary tract specimens, respectively. These quantitation criteria are still subject to discussion, and this study specifically aims to validate the quantitation criterion of HGUC in lower urinary tract.DesignThe authors reviewed two cohorts of lower urinary tract cases. The first cohort consisted of 100 liquid‐based ThinPrep slides with the diagnosis of HGUC having positive histology on concurrent or follow‐up biopsies within 3 months. The second cohort was 36 HGUC cases with negative histology on concurrent biopsies and within 3 months. The number of high‐grade cells (HGCs) meeting the TPS qualitative criteria were counted under the light microscope driven in a grid‐like manner.ResultsThe first 100 urine samples showed five cases (5.0%) with three HGCs, three cases (3.0%) had four HGCs, five cases (5.0%) showed five HGCs, and 25 cases (25.0%) had between 6–10 HGCs. The risk of high‐grade malignancy (ROHM) in cases with five or more HGCs was 100%, whereas those with three HGCs was 60.0%. The second cohort of HGUC was considered “positive” despite a negative histology.ConclusionThis study confirms that quantitation is an essential key to diagnose HGUC. The current TPS criterion of a minimum of five malignant cells in lower tract is robust with a ROHM of 100%. Diagnosing HGUC with less than five HGCs runs the risk of lowering the ROHM.

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Enzyme-labeled liquid-based cytology (ELLBC): a new noninvasive diagnostic method for bladder cancers

Jiang,

Li,

Chen

et al. 2024

J Cancer Res Clin Oncol

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Background Based on liquid-based cytology, we performed an enzyme histochemical staining using acid phosphatase as a marker and termed it ELLBC. The aim of this study was to investigate the value of ELLBC in the diagnosis of bladder cancer. Methods Fifty patients who were initially diagnosed with suspected bladder cancers (hematuria or bladder irritation symptoms, urinary ultrasound suggestive of bladder mass) at the Second Affiliated Hospital of Anhui Medical University (Anhui, China) from January 2022 to December 2022 were selected as the study subjects, all of whom underwent ELLBC, CC, and histopathology Histopathology was used as the gold standard to calculate the diagnostic efficacy of ELLBC, CC and ELLBC combined with CC in bladder cancer. Results Histopathological examination revealed 35 positive cases in 50 patients, including 15 cases of high-grade uroepithelial carcinoma (HGUC) and 20 cases of low-grade uroepithelial carcinoma (LGUC.) The sensitivity of ELLBC was 82.86%, the specificity was 93.33%, the positive predictive value (PPV) was 96.67%, the negative predictive value (NPV) was 70.00%, and the accuracy was 86.00%; CC had a sensitivity of 37.14%, specificity of 80.00%, PPV of 81.25%, NPV of 35.29%, and accuracy of 50%; ELLBC combined with CC had a sensitivity of 88.57%, specificity of 73.33%, PPV of 88.57%, NPV of 73.33%, and accuracy of 84.00%. The sensitivity and specificity of ELLBC were higher than that of CC, and the difference was statistically significant (p < 0.05), ELLBC combined with CC achieved higher sensitivity, but the diagnostic accuracy decreased. For clinical staging, the diagnostic accuracy was 86.36% for ELLBC and 40.91% for CC in patients in Stage I, and 90.91% for ELLBC and 36.36% for CC in patients in Stage II. Conclusion ELLBC has high clinical application value for the diagnosis of bladder cancer and can provide new options and methods for the early screening of bladder cancer.

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Comparison of conventional and liquid‐based cytology using The Paris System for Reporting Urinary Cytology

Cited by 3 publications

References 24 publications

Cytospin performance when using Paris system for reporting urinary cytology

Cytospin performance when using Paris system for reporting urinary cytology

Are we on track for diagnosing high‐grade urothelial carcinoma with a minimum quantity of five malignant cells in lower tract specimens? Critical analysis of The Paris System Quantitation Criteria

Enzyme-labeled liquid-based cytology (ELLBC): a new noninvasive diagnostic method for bladder cancers

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