Comparison of Complete Blood Count Parameters between Venous and Capillary Blood in Oncology Patients

Chavan, Preeti; Bhat, Vivek; Tiwari, Manikchandra; Gavhane, Umakant; Pal, Sanjay

doi:10.4103/0974-2727.176238

Cited by 12 publications

(13 citation statements)

References 5 publications

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“…Capillary and venous blood compartments have been studied for differences in measurements of hematocrit, leukocyte populations, glucose, G6PD-deficiency status, and other analytes [5–15]. Results of these studies vary, for example from finding slightly higher leukocyte counts in capillary than venous samples to platelet counts being higher in venous than capillary blood [5, 6, 8–10]. Notably, certain species of another intraerythrocytic parasite, Babesia , have been found to exhibit higher parasite density levels in capillary than in venous compartments [16, 17].…”

Section: Introductionmentioning

confidence: 99%

Comparison on simultaneous capillary and venous parasite density and genotyping results from children and adults with uncomplicated malaria: a prospective observational study in Uganda

Lehane¹,

Were

Wade³

et al. 2019

BMC Infect Dis

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Background Blood smear microscopy remains the gold-standard method to diagnose and quantify malaria parasite density. In addition, parasite genotyping of select loci is the most utilized method for distinguishing recrudescent and new infections and to determine the number of strains per sample. In research settings, blood may be obtained from capillary or venous compartments, and results from these matrices have been used interchangeably. Our aim was to compare quantitative results for parasite density and strain complexity from both compartments. Methods In a prospective observational study, children and adults presenting with uncomplicated Plasmodium falciparum malaria, simultaneous capillary and venous blood smears and dried blood spots were collected over 42-days following treatment with artemether-lumefantrine. Blood smears were read by two microscopists, any discrepancies resolved by a third reader. Parasite DNA fingerprinting was conducted using six microsatellites. Bland Altman analysis and paired t-test/McNemar’s test were used to assess the difference in density readings and measurements. Results Two hundred twenty-three participants were included in the analysis (177 children (35 HIV-infected/142 HIV-uninfected), 21 HIV-uninfected pregnant women, and 25 HIV-uninfected non-pregnant adults). Parasite density measurements did not statistically differ between capillary and venous blood smears at the time of presentation, nor over the course of 42-day follow-up. Characterization of merozoite surface protein-2 (MSP-2) genetic polymorphism demonstrated a higher level of strain diversity at the time of presentation in venous samples, as compared with capillary specimens ( p = 0.02). There was a high degree of variability in genotype-corrected outcomes when pairs of samples from each compartment were compared using MSP-2 alone, although the variability was reduced with the use of multiple markers. Conclusions Parasite density measurements do not statistically differ between capillary and venous compartments in all studied demographic groups at the time of presentation with malaria, or over the course of follow-up. More strains were detected by MSP-2 genotyping in venous samples than in capillary samples at the time of malaria diagnosis. The use of multiple polymorphic markers reduces the impact of variability in strain detection on genotype-corrected outcomes. This study confirms that both capillary and venous compartments can be used for sampling with confidence in the clinical research setting. Trial registration The trial was registered at ClinicalTrials.gov under registration no. NCT01717885 . Electronic supplementary material The online version of this article (10.1186/s12879-019-4174-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Comparison on simultaneous capillary and venous parasite density and genotyping results from children and adults with uncomplicated malaria: a prospective observational study in Uganda

Lehane¹,

Were

Wade³

et al. 2019

BMC Infect Dis

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“…Differences in capillary and venous Haemoglobin counts is long established but the mechanism for this is unknown 23 . WCC and platelets have been similarly affected, with some studies describing non-significant differences 24 and some recognising that there are differences 25 with greater precision achievable with venous blood 23 . As in our study, good correlation has been observed in the literature with capillary and venous comparisons of WCC albeit in different patient populations such as oncology 24 or healthy athletes 26 .…”

Section: Discussionmentioning

confidence: 99%

Utilisation of Remote Capillary Blood Testing in an Outpatient Clinic Setting to improve shared decision making and patient and clinician experience: a validation and pilot study

Nwankwo

McLaren

Donovan

et al. 2020

Preprint

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Background In a tertiary respiratory centre, large cohorts of patients are managed in an outpatient setting and require blood tests to monitor disease activity and organ toxicity. This requires either visits to tertiary centres for phlebotomy and physician review or utilisation of primary care services. Objectives This study aims to validate remote capillary blood testing in an outpatient setting and analyse impact on clinical pathways. Methods A single-centre prospective cross-sectional validation and parallel observational study was performed. Remote finger prick capillary blood testing was validated compared to local standard venesection using comparative statistical analysis: paired t-test, correlation and Bland-Altman. Capillary was considered interchangeable with venous samples if all 3 criteria were met: non-significant paired t-test (i.e. p>0.05), Pearson's correlation coefficient (r) >0.8 and 95% of tests within 10% difference through Bland-Altman (Limits of agreement). In parallel, current clinical pathways including phlebotomy practice was analysed over 4 weeks to review test predictability. A subsequent pilot cohort study analysed potential impact of remote capillary blood sampling on shared decision making and outpatient clinical pathways. Results 117 paired capillary and venous blood samples were prospectively analysed. Interchangeability with venous blood was seen with HbA1c (%), total protein and CRP. Further tests, although not interchangeable, are likely useful to enable longitudinal remote monitoring (e.g. liver function, total IgE, and vitamin D). 65% of outpatient clinic blood tests were predictable with 16% of patients requiring further contact due to actions required. Pilot implementation of remote capillary sampling showed patient and clinician-reported improvement in shared decision-making given contemporaneous blood test results. Conclusions Remote capillary blood sampling can be used accurately for specific tests to monitor chronic disease, and when incorporated into an outpatient clinical pathway can improve shared decision making and patient experience. Further research is required to determine health-economic impact and applicability within telemedicine-based outpatient care.

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“…Of great importance is that this study evaluated bias across low to high concentrations of medical decisions for all the parameters, and, given that FBC is a crucial laboratory investigation for diagnosis, case management and in clinical trials [3], replacement or backup laboratory methods for this test should have high level of agreement and any observed bias should be clinically acceptable.…”

Section: Discussionmentioning

confidence: 99%

“…Full Blood Count (FBC) also referred to as Complete Blood Count (CBC) is an important laboratory investigation required for disease diagnosis, patient management, subject screening and safety testing for vaccine or drugs during clinical trials [1][2][3]. A recent assessment of all laboratory test requests made from general practice in Oxfordshire, UK, showed FBC as the second most commonly requested blood tests [4].…”

Section: Introductionmentioning

confidence: 99%

Full Blood Count Estimation Using Abbott Cell-Dyn Ruby 5-Parts in Comparison with Boule Medonic M-Series 3-Parts Differential Haematology Analyser

Lawal¹

2020

AJBSR

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Full Blood Count (FBC) is a significant laboratory investigation often requested in clinical care and trials; laboratories should have comparable alternative methods for this test to ensure uninterrupted services. This study evaluated to what extent the Abbott CELL-DYN Ruby and Boule Medonic M-series haematology analysers can be used interchangeably. Using 110 leftover EDTA blood samples to test twelve haematological parameters, correlations and bias between the two analysers for each blood parameter were determined by plotting Passing & Bablok and Bland-Altman's plots respectively. The two analysers were assessed comparable for a test when the bias with the 95% CI falls within the clinically acceptable limits which had been defined a priori. All the twelve FBC parameters showed strong positive correlations between the measurements from the two analysers. The measurements on both analysers however showed some bias for all the parameters evaluated except Red Blood Cells (RBC), whose bias was 0.00 x 10-12/L (-0.01, 0.02). The observed biases met the acceptance criteria, except Haematocrit (HCT), Mean Cell Volume (MCV) and Lymphocytes -% (Lym-%). Abbott CELL-DYN Ruby and Boule Medonic M-series haematology analysers are comparable for Haemoglobin (HGB), White Blood Cell Count (WBC), RBC, Platelets (PLT), Mean Cell Haemoglobin (MCH), Mean Cell Haemoglobin Concentration (MCHC), Red Cell Distribution Width (RDW), Mean Platelets Volume (MPV) and Lymphocytes -Absolute (Lym-Abs), but not for HCT, MCV, and LYM which will require interpreting results using equipment-specific reference intervals. The results of this study may help laboratories and clinics in selecting comparable methods for FBC. Moreover, the two study analysers have closed sampling facilities which make them safe for handling suspected infectious samples, as contact with personnel is minimal.

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Comparison of Complete Blood Count Parameters between Venous and Capillary Blood in Oncology Patients

Cited by 12 publications

References 5 publications

Comparison on simultaneous capillary and venous parasite density and genotyping results from children and adults with uncomplicated malaria: a prospective observational study in Uganda

Comparison on simultaneous capillary and venous parasite density and genotyping results from children and adults with uncomplicated malaria: a prospective observational study in Uganda

Utilisation of Remote Capillary Blood Testing in an Outpatient Clinic Setting to improve shared decision making and patient and clinician experience: a validation and pilot study

Full Blood Count Estimation Using Abbott Cell-Dyn Ruby 5-Parts in Comparison with Boule Medonic M-Series 3-Parts Differential Haematology Analyser

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