Comparison of Clot-Based vs Chromogenic Factor Xa Procoagulant Phospholipid Activity Assays

Bohling, Sandra D.; Pagano, Monica B.; Stitzel, Maria R.; Ferrell, Chris; Yeung, W.; Chandler, Wayne L.

doi:10.1309/ajcpgsj4nhfqmx9w

Cited by 14 publications

(12 citation statements)

References 18 publications

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“…The contribution of PS to procoagulant activity can be measured in clot-based or chromogenic assays that provide functional measurements of procoagulant phospholipids [178]. …”

Section: Quality Of Platelet Concentratesmentioning

confidence: 99%

Quality Assessment of Established and Emerging Blood Components for Transfusion

Acker

Marks

Sheffield

2016

Journal of Blood Transfusion

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Blood is donated either as whole blood, with subsequent component processing, or through the use of apheresis devices that extract one or more components and return the rest of the donation to the donor. Blood component therapy supplanted whole blood transfusion in industrialized countries in the middle of the twentieth century and remains the standard of care for the majority of patients receiving a transfusion. Traditionally, blood has been processed into three main blood products: red blood cell concentrates; platelet concentrates; and transfusable plasma. Ensuring that these products are of high quality and that they deliver their intended benefits to patients throughout their shelf-life is a complex task. Further complexity has been added with the development of products stored under nonstandard conditions or subjected to additional manufacturing steps (e.g., cryopreserved platelets, irradiated red cells, and lyophilized plasma). Here we review established and emerging methodologies for assessing blood product quality and address controversies and uncertainties in this thriving and active field of investigation.

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“…The contribution of PS to procoagulant activity can be measured in clot-based or chromogenic assays that provide functional measurements of procoagulant phospholipids [178]. …”

Section: Quality Of Platelet Concentratesmentioning

confidence: 99%

Quality Assessment of Established and Emerging Blood Components for Transfusion

Acker

Marks

Sheffield

2016

Journal of Blood Transfusion

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“…For example, a recent study has reported that larger microparticles show higher levels of procoagulant phospholipid activity than smaller microparticles, probably due to their greater surface area [31]. The size of the microparticles may have an effect on their function.…”

Section: Definition Of Microparticlesmentioning

confidence: 99%

Microparticle counts in platelet-rich and platelet-free plasma, effect of centrifugation and sample-processing protocols

Chandler

2013

Blood Coagulation &Amp; Fibrinolysis

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This study provides the first estimates of microparticle numbers in platelet-rich plasma (PRP) from normal individuals, closer to in-vivo levels, using higher-resolution flow cytometry. We measured platelet (CD41+) and annexin V+ microparticles in fresh and frozen aliquots of PRP, platelet-poor plasma, platelet-free plasma (PFP), and microparticles isolated by high-speed centrifugation. PRP from healthy individuals contained 730,000/μl total microparticles based on light-scattering measurements. A median of 27,000/μl microparticles in PRP were of platelet origin and 120,000/μl annexin V+, and of these, 24,000/μl were dual-positive procoagulant platelet microparticles. Double centrifugation of PRP removed 99% of platelets, but also 80% of annexin V+ CD41+, 93% of annexin V+ CD41-, and 58% of annexin V- CD41+ microparticles. Loss of microparticles with centrifugation varied from individual to individual. Microparticle counts after isolation by centrifugation and double washing were not significantly different than counts in the original PFP sample, but lower than in PRP. Freeze-thawing of PFP had no effect on platelet microparticle counts, but slightly increased annexin V+, CD41- counts. Freeze-thawing of isolated washed microparticles resulted in a 30-50% increase in annexin V+ microparticles. PRP contains large numbers of cellular microparticles, including platelet and annexin V+ microparticles, which are lost to varying degrees when PRP is double centrifuged to remove platelets. Microparticles remaining in PFP can be recovered by high-speed centrifugation without loss compared to the original PFP sample. Freeze-thawing has variable effects on microparticle counts depending on the sample preparation used.

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“…[4,5]. Therefore, detection and measurement of MPs have a potential application in the study of different pathologies, as well as a prognostic role and could serve as a tool in monitoring effectiveness of therapy in the setting of major diseases.…”

mentioning

confidence: 99%

“…Increased procoagulant phospholipid activity (PPA) of MPs is associated with many pathological states, including cancer, cardiovascular disease, infection and inflammatory diseases, as well as other clinical states associated with platelet activation. [4,5]. Therefore, detection and measurement of MPs have a potential application in the study of different pathologies, as well as a prognostic role and could serve as a tool in monitoring effectiveness of therapy in the setting of major diseases.…”

mentioning

confidence: 99%