Comparison of clinical performance of antigen basedenzyme immunoassay (EIA) and major outer membrane protein (MOMP)-PCR for detection of genital Chlamydia trachomatis infection

Rostami, Mahmoud Nateghi; Rashidi, Batool Hossein; Aghsaghloo, Fatemeh; Nazari, Razieh

doi:10.29252/ijrm.14.6.411

Cited by 8 publications

(4 citation statements)

References 30 publications

(28 reference statements)

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“…However, due to disappointing low sensitivity it cannot be recommended for the diagnosis of an acute chlamydial infection. Our findings are in accordance with findings from other studies where low sensitivity (20-60%) of rapid tests was observed [18][19][20][21][22]. Moreover, the study of Nateghi Rostami et al suggest primary screening of chlamydial infection in women by the low-cost EIA, but confirmation of the negative results by a DNA amplification method is required because of low sensitivity of EIA assays [23].…”

Section: Discussionsupporting

confidence: 92%

Chlamydia trachomatis screening in resource-limited countries – Comparison of diagnostic accuracy of 3 different assays

Tošić-Pajić

Sazdanović

Sorak

et al. 2018

J Infect Dev Ctries

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Introduction: Commercially available assays were evaluated in order to determine diagnostic accuracy of Chlamydia trachomatis specific tests for screening. Methods: The study included 225 sexually active men and women, who were tested for genital chlamydial infection in Institute of Public Health Kragujevac. Three screening tests were used: direct immunofluorescence (DIF) and rapid lateral immunochromatographic test (RT) for qualitative detection of chlamydial antigens and immunoenzyme (ELISA) test for detection of serum levels of anti-chlamydial IgA and IgG antibodies. Diagnostic efficiency of these tests were determined in relation to results obtained by RT-PCR method. Results: Statistical significance between the results obtained by RT-PCR as a gold standard and DIF, RT and ELISA were analyzed using chi-square (χ2) test. Statistical analysis showed a significant difference between RT-PCR and analyzed screening tests: DIF (χ2 = 303; p < 0.001), RT (χ2 = 4.19; p = 0.041), serum IgA (χ2 = 4.19; p = 0.041) and serum IgG (χ2 = 67; p < 0.001) which indicates poor agreement between these tests. Large numbers of false positive (FP) and false negative (FN) results were observed for all tested assays. According to Youden’s index, serum IgG and DIF testing demonstrated the most-balanced sensitivity-specificity rate. RT assay exhibits the highest expanded Youden’s index, as well as the best overall diagnostic accuracy. Conclusions: None of evaluated screening tests can be recommended as individual method for the diagnosis of acute infection. We suppose that RT-PCR is unlikely to be a cost-effective screening strategy within the Serbian health system.

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Section: Discussionsupporting

confidence: 92%

Chlamydia trachomatis screening in resource-limited countries – Comparison of diagnostic accuracy of 3 different assays

Tošić-Pajić

Sazdanović

Sorak

et al. 2018

J Infect Dev Ctries

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“…In a previous study we found that antigen based enzyme immunoassay for C. trachomatis has 59.5% sensitivity [21]. In the present study, prevalence rate of C. trachomatis was 11.67% by PCR, while ienzume immunoassay demonstrates prevalence rate of 7.14% in women of the same region [21].…”

Section: Discussionsupporting

confidence: 49%

“…For molecular identification of C. trachomatis in genital specimens, ompI target gene encoding for chlamydial major outer membrane protein (MOMP) was amplified by nested PCR as previously described [21]. The sequence of external primers (Ex-p1/Ex-p2) and nested primers (In-p1/In-p2) are shown in Table 1 (CinnaGene, Tehran, Iran).…”

Section: Detection Of C Trachomatis Infectionmentioning

confidence: 99%

A multiplex assay of Trichomonas vaginalis, Chlamydia trachomatis and Neisseria gonorrhoeae infections in genital specimens

Rostami

Rashidi

Nazari

et al. 2017

J Infect Dev Ctries

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Introduction: A significant proportion of patients with Sexually Transmitted Infections (STIs) are coinfected with multiple pathogens. We report here development of a multiplex PCR for simultaneous detection of Chlamydia trachomatis (C.trachomatis), Neisseria gonorrhoeae (N.gonorrhoeae) and Trichomonas vaginalis (T.vaginalis) in genital specimens from women. Methodology: After detection of the organisms by routine techniques including PCR, culture and direct smear, multiplex-PCR was optimized to detect ompI gene of CT, parC of NG, ITS ribosomal RNA of TV as target genes. The limit of detection (LOD) was determined using serially diluted genomic DNA from known number of each pathogen. Results: Totally 300 volunteers with mean age of 36.5 ±7.03 years were included and 266 (88.7%) had genitourinary clinical manifestations. Of 300 women, 150 (50.0%) were infected. Of them, 17 (5.7%) had N. gonorrhoeae, 98 (32.7%) T. vaginalis and 35 (11.7%) C. trachomatis. Multiplex-PCR revealed a total of 10 coinfections (3.3%) including 2 specimens of C. trachomatis/ N. gonorrhoeae, 3 specimens of C .trachomatis/ T. vaginalis and 5 specimens of N. gonorrhoeae/T. vaginalis coinfections. The sensitivity and specificity of multiplex-PCR for detecting N. gonorrhoeae were 100% and 98.59% (279 of 283) respectively and, for C. trachomatis and T. vaginalis were 100%. The LOD was 0.1 pg of DNA for C. trachomatis and N. gonorrhoeae, and 1.5 pg for T. vaginalis. Conclusions: The performance of this multiplex-PCR makes it a sensitive, rapid and affordable technique in clinical laboratory for simultaneous detection of STIs.

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“…It also plays a role in pathogenesis and possibly adhesion. The MOMP along with the lipopolysaccharide, makes up the surface of the elementary body cell (Collar et al 2022;Rostami et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Detection of aac(3)-I gene in Chlamydia trachomatis isolated from conjunctivitis by using PCR technique in Al-Najaf province, Iraq

Al-Buhamrah,

Hussain,

Saadon

2024

MBJ

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Chlamydia trachomatis causes conjunctivitis to spread throughout the world, it infects people of all ages and can cause blindness if not treated properly. Therefore, the current study aimed to diagnose C. trachomatis that causes conjunctivitis and identify the detection of the aac(3)-I gene in C. trachomatis isolated from conjunctivitis by using Polymerase chain reaction (PCR ) technique. One hundred and seven swabs were collected from patients infected with conjunctivitis from different ages (1 month -70 years) and for both sexes (63 samples from females and 44 samples from males) for the period from 27 November till 19 May 2023 from Al-Najaf province in Iraq. Purulent material from the conjunctiva sac was collected on a sterile swab and placed in phosphate-buffered saline and incubated in a deep freezer after completing the collection. All samples were subjected to a PCR to detect the major outer membrane protein (MOMP) gene to diagnose the Chlamydia trachomatis. where the results showed that out of 107 samples, 26 (24.3%) were positive for C. trachomatis. The individuals infected with C. trachomatis were categorised into multiple age groups. The age group ranging from one month to 14 years exhibited the highest prevalence of conjunctivitis. The age group between 15-28 years was followed by the age group between 57-70 years, while the lowest group for conjunctivitis was the age group between 43-56. The existence of the aac(3)-I gene, which confers resistance to the antibiotic gentamicin routinely employed for the treatment of eye infections, was identified. Among the 26 isolates of C. trachomatis, 15 (57.69%) were found to have the aac(3)-I gene. The present investigation demonstrated the significant involvement of C. trachomatis in conjunctivitis, with a higher susceptibility observed among women. The prevalence of conjunctivitis was higher in the age range of 1-28 compared to other age groups. The present investigation exhibited the dissemination of the gentamicin resistant aac(3)-I gene among C. trachomatis bacteria.

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Comparison of clinical performance of antigen basedenzyme immunoassay (EIA) and major outer membrane protein (MOMP)-PCR for detection of genital Chlamydia trachomatis infection

Cited by 8 publications

References 30 publications

Chlamydia trachomatis screening in resource-limited countries – Comparison of diagnostic accuracy of 3 different assays

Chlamydia trachomatis screening in resource-limited countries – Comparison of diagnostic accuracy of 3 different assays

A multiplex assay of Trichomonas vaginalis, Chlamydia trachomatis and Neisseria gonorrhoeae infections in genital specimens

Detection of aac(3)-I gene in Chlamydia trachomatis isolated from conjunctivitis by using PCR technique in Al-Najaf province, Iraq

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