Comparison of CHROMagar Salmonella Medium and Xylose-Lysine-Desoxycholate and Salmonella-Shigella Agars for Isolation of
            <i>Salmonella</i>
            Strains from Stool Samples

Maddocks, Susan; Olma, Tom; Chen, Sharon

doi:10.1128/jcm.40.8.2999-3003.2002

Cited by 38 publications

(30 citation statements)

References 9 publications

(6 reference statements)

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“…Salmonella isolates in dairy cows were serotyped as S. Kentucky (8,20:i:z 6 ) (n = 3), S. Anatum (3,10, [15], [15,34]:e,h:1,6) (n = 1), and S. Typhimurium (1,4, [5],12:i:1,2) (n = 1). In addition, 1 isolate in buffalo was S. Kentucky (8,20:i:z 6 ) and the 1 isolate in camel was S. Abony (1,4, [5],12,27:b:e,n,x) ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Salmonella isolates in calves were serotyped as S. Kentucky (8,20:i:z 6 ) (n = 19), S. Muenchen (6,8:d:1,2) (n = 5), S. Gaminare (16:d:1,7) (n = 4), S. Anatum (3,10, [15], [15,34]:e,h:1,6) (n = 3), S. Enteritidis (1, 9,12: g,m:-) (n = 1), and S. Muenster (3,10[15] [15,34]:e,h:1,5) (n = 1). Salmonella isolates in dairy cows were serotyped as S. Kentucky (8,20:i:z 6 ) (n = 3), S. Anatum (3,10, [15], [15,34]:e,h:1,6) (n = 1), and S. Typhimurium (1,4, [5],12:i:1,2) (n = 1).…”

Section: Resultsmentioning

confidence: 99%

“…Infected animals may spread the agent through feces without showing any clinical symptoms (23). For Salmonella isolation, three cultural procedure stages are generally used: preenrichment, selective enrichment, and selective culture (15,16). In the preenrichment stage, the most commonly used media are buffered peptone water (BPW) and lactose broth (15).…”

Section: Discussionmentioning

confidence: 99%

“…For Salmonella isolation, three cultural procedure stages are generally used: preenrichment, selective enrichment, and selective culture (15,16). In the preenrichment stage, the most commonly used media are buffered peptone water (BPW) and lactose broth (15). In selective enrichment, media that support production of Salmonella and inhibit production of other bacteria are used.…”

Section: Discussionmentioning

confidence: 99%

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Serotypes of Salmonella isolated from feces of cattle, buffalo,and camel and sensitivities to antibiotics in Turkey

Hadimli¹,

Pinarkara²,

Sakmanoğlu³

et al. 2017

Turk J Vet Anim Sci

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IntroductionSalmonellosis is a common bacterial enteric infection with significant economic losses for the intensive production of cattle, sheep, and poultry (1,2). Salmonella species are zoonotic and are transmitted to humans via ingestion of contaminated milk, eggs, and meat (3,4). Although Salmonella infections may occur at any age in cattle, the associated clinical symptoms are more severe in calves from the first 2 weeks to 3 months of their life (5,6).Salmonella enterica subsp. enterica may cause infections associated with several clinical symptoms or systemic infections characterized by diarrhea and septicemia and may even lead to death in severe cases. It can be harbored by asymptomatic carriers (2,5). In the enteric form of salmonellosis, the stool is sticky and watery and has a putrid odor. It may contain flecks of mucus, shreds of the mucous membrane, and in some cases blood. Young calves and lambs frequently develop septicemia. Furthermore, marked depression, fever, symptoms of the central nervous system, pneumonia, and death within 2-3 days can also occur (7-9). Clinical symptoms and necropsy findings alone are not sufficient for a definitive diagnosis of Salmonella infection. It is imperative to isolate and identify the causative Salmonella species (10-12). While the isolation of Salmonella is relatively easy via bacterial culture from samples taken from animals with septicemia, carcasses, and the organs of aborted fetuses, enrichment is needed to increase the chance of isolating Salmonella from feed samples or from fecal cultures used to detect carriers (7,13,14).This study aimed to isolate and serotype Salmonella species from fecal samples of dairy cattle, calves with diarrhea, camels, and water buffaloes and to determine sensitivities of the isolates to antibiotics. Materials and methods SamplingA total of 869 fecal samples were collected from 21 herds in 13 provinces (Konya,

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Serotypes of Salmonella isolated from feces of cattle, buffalo,and camel and sensitivities to antibiotics in Turkey

Hadimli¹,

Pinarkara²,

Sakmanoğlu³

et al. 2017

Turk J Vet Anim Sci

View full text Add to dashboard Cite

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“…MLCB is used principally for Salmonella detection in food and environmental samples, due to its high selectivity (Nye et al, 2002;van Schothorst et al, 1987). On the other hands, XLD is the most commonly used medium for isolating S. enterica (Nye et al, 2002), and CAS is superior to other selective agars for isolating Salmonella from stool samples (Gailot et al, 1999;Maddocks et al, 2002;Perez et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Rapid Quantitative Detection of Salmonella enterica Using Fluorescence In Situ Hybridization with Filter-cultivation (FISHFC) Method

Shimizu

Aoi

Osanai

et al. 2013

FSTR

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Specific detection and enumeration of Salmonella enterica in food using conventional culture-based methods (CCBM) are time consuming and labor intensive. This study was conducted to develop a rapid S. enterica detection and enumeration method by combining fluorescence in situ hybridization (FISH) with micro-colony formation culture (FISHFC). Specificity tests of the SAL343 probe for S. enterica detection revealed that SAL343-associated fluorescent micro-colonies were observed specifically for S. enterica, but not for any other organisms. This finding suggests that SAL343 is highly specific for detecting S. enterica using FISHFC. For validation, FISHFC with SAL343 was compared to CCBM, with multiple selective agar, using spiked food samples; no significant differences in enumeration were found between FISHFC and CCBM (p > 0.05). The FISHFC method allowed enumeration of S. enterica within 10 h while CCBM allowed enumeration within 5 days. Therefore, the FISHFC method has potential application for more rapid and specific enumeration of S. enterica in food samples compared to other available methods.Keywords: Salmonella enterica, fluorescence in situ hybridization, FISHFC, rapid detection *To whom correspondence should be addressed. E-mail: yamasaki@fish.hokudai.ac.jp IntroductionSalmonella are Gram-negative, facultatively anaerobic, rod-shaped bacteria that exist ubiquitously in the environment and are commonly found in water, animal intestine, reptilian skin and food. Salmonella also causes salmonellosis in humans worldwide due to the consumption of contaminated food such as fresh fruits and vegetables (Matthews, 2006). The genus Salmonella currently consists of two species: S. enterica and S. bongori. S. enterica is comprised of six subspecies: enterica, salamae, arizonae, diarizonae, houtenae and indica, which are further subdivided into more than 2,500 serotypes (Agbaja et al., 2011;Su and Chiu, 2007). Most of the Salmonella responsible for disease in humans and animals are members of S. enterica. S. enterica serotype Enteritidis and Typhimurium are two of the main sources of human food poisoning. Although S. bongori has been reported to infect humans, it is generally associated with cold-blooded animals (Giammanco et al., 2002). Therefore, S. enterica are clinically important pathogens of humans.Routine methods for enumerating Salmonella are traditional microbiological methods based on plating using selective agar and biochemical identification. However, the biochemical characteristics of some isolates may differ from the common phenotypic properties of typical strains. Moreover, it is recommended that multiple selective agars be used for Salmonella detection in food samples (Nye et al., 2002). Thus, the conventional methods for detecting Salmonella are time-consuming and labor-intensive, taking at least several days to obtain the final results (Kim et al., 2007). To overcome technical problems associated with Salmonella detection, a number of molecular-based methods, such as PCR, quantitative PCR (qPCR), enzy...

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Determination of Pathogens in Greywater

Al‐Gheethi

Noman

Kassim

2018

Management of Greywater in Developing Countries

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Comparison of CHROMagar Salmonella Medium and Xylose-Lysine-Desoxycholate and Salmonella-Shigella Agars for Isolation of Salmonella Strains from Stool Samples

Cited by 38 publications

References 9 publications

Serotypes of Salmonella isolated from feces of cattle, buffalo,and camel and sensitivities to antibiotics in Turkey

Serotypes of Salmonella isolated from feces of cattle, buffalo,and camel and sensitivities to antibiotics in Turkey

Rapid Quantitative Detection of Salmonella enterica Using Fluorescence In Situ Hybridization with Filter-cultivation (FISHFC) Method

Determination of Pathogens in Greywater

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