Comparison of Chondrogensis in Static and Perfused Bioreactor Culture

Pazzano, David; Mercier, Kathi A.; Moran, John M.; Fong, Stephen S.; DiBiasio, David; Rulfs, Jill; Kohles, Sean S.; Bonassar, Lawrence J.

doi:10.1021/bp000082v

Cited by 246 publications

(194 citation statements)

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“…Shear stress of up to 1 dyn cm −2 (average velocity of up to 640 μm s −1 ) increased deposition of mineralized matrix by marrow stromal osteoblasts of a tissue engineered bone in a dose-dependent manner 26,27 . Similarly, perfusion cin the range from 1 to 170 μm s −1 increased the content of DNA, glycosaminoglycans and hydroxyproline of a tissue-engineered cartilage compared to the static controls [28][29][30] . The appropriate average velocities also depend on the cell type being cultivated 23,25 .…”

Section: Selecting Culture Parametersmentioning

confidence: 99%

Cardiac tissue engineering using perfusion bioreactor systems

et al. 2008

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This protocol describes tissue engineering of synchronously contractile cardiac constructs by culturing cardiac cell populations on porous scaffolds (in some cases with an array of channels) and bioreactors with perfusion of culture medium (in some cases supplemented with an oxygen carrier). The overall approach is 'biomimetic' in nature as it tends to provide in vivo-like oxygen supply to cultured cells and thereby overcome inherent limitations of diffusional transport in conventional culture systems. In order to mimic the capillary network, cells are cultured on channeled elastomer scaffolds that are perfused with culture medium that can contain oxygen carriers. The overall protocol takes 2-4 weeks, including assembly of the perfusion systems, preparation of scaffolds, cell seeding and cultivation, and on-line and end-point assessment methods. This model is well suited for a wide range of cardiac tissue engineering applications, including the use of human stem cells, and highfidelity models for biological research.

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Section: Selecting Culture Parametersmentioning

confidence: 99%

Cardiac tissue engineering using perfusion bioreactor systems

et al. 2008

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“…It has been shown that the functional development of a tissue engineered cartilage construct is governed to a large extent by the bioreactor environment to which it is exposed. The main types of bioreactors that have been employed in cartilage TE studies include static or orbitally shaken Petri dishes, mixed culture in spinner flasks, concentric cylinder bioreactors, rotating wall vessel (RWV) bioreactors, and perfusion bioreactors (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). Static culture tends to produce constructs with an inhomogeneous matrix distribution, with enhanced deposition in the periphery.…”

Section: Introductionmentioning

confidence: 99%

“…This resulted in the lowest permeability and the highest equilibrium, which are among the most important parameters that determine the suitability of a tissue engineered construct. In general perfusion through or around constructs enhances matrix synthesis (5,6,10), although it has also been reported that matrix production can be inhibited (7). In addition, bioreactors have been specifically designed to incorporate some form of mechanical stimulation, such as fluid shear stress, hydrostatic pressure, and dynamic compression, which have been found to be beneficial for cartilage matrix biosynthesis (12)(13)(14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Computational Study of Culture Conditions and Nutrient Supply in Cartilage Tissue Engineering

Sengers

Donkelaar

Oomens

et al. 2008

Biotechnol Progress

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Different culture conditions for cartilage tissue engineering were evaluated with respect to the supply of oxygen and glucose and the accumulation of lactate. A computational approach was adopted in which the culture configurations were modeled as a batch process and transport was considered within constructs seeded at high cell concentrations and of clinically relevant dimensions. To assess the extent to which mass transfer can be influenced theoretically, extreme cases were distinguished in which the culture medium surrounding the construct was assumed either completely static or well mixed and fully oxygenated. It can be concluded that severe oxygen depletion and lactate accumulation can occur within constructs for cartilage tissue engineering. However, the results also indicate that transport restrictions are not insurmountable, providing that the medium is well homogenized and oxygenated and the construct's surfaces are sufficiently exposed to the medium. The large variation in uptake rates of chondrocytes indicates that for any specific application the quantification of cellular utilization rates, depending on the cell source and culture conditions, is an essential starting point for optimizing culture protocols.

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“…9,10 However, the conventional threedimensional culture system that is essential for chondrogenic induction has specific problems including the effects of gravitational stress on the cells and nutrition and excretion deficiencies. 11 A rotating wall vessel (RWV) bioreactor ( Fig. 1), which was developed in a NASA space experiment, is an effective culture system that utilizes a circular vessel with a gas-exchange membrane and rotates vertically to provide culture medium flow and to balance gravity.…”

mentioning

confidence: 99%

Rotating three‐dimensional dynamic culture of adult human bone marrow‐derived cells for tissue engineering of hyaline cartilage

Sakai

Mishima

Ishii

et al. 2009

Journal Orthopaedic Research

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The method of constructing cartilage tissue from bone marrow-derived cells in vitro is considered a valuable technique for hyaline cartilage regenerative medicine. Using a rotating wall vessel (RWV) bioreactor developed in a NASA space experiment, we attempted to efficiently construct hyaline cartilage tissue from human bone marrow-derived cells without using a scaffold. Bone marrow aspirates were obtained from the iliac crest of nine patients during orthopedic operation. After their proliferation in monolayer culture, the adherent cells were cultured in the RWV bioreactor with chondrogenic medium for 2 weeks. Cells from the same source were cultured in pellet culture as controls. Histological and immunohistological evaluations (collagen type I and II) and quantification of glycosaminoglycan were performed on formed tissues and compared. The engineered constructs obtained using the RWV bioreactor showed strong features of hyaline cartilage in terms of their morphology as determined by histological and immunohistological evaluations. The glycosaminoglycan contents per mg DNA of the tissues were 10.01 AE 3.49 mg/mg DNA in the case of the RWV bioreactor and 6.27 AE 3.41 mg/mg DNA in the case of the pellet culture, and their difference was significant. The RWV bioreactor could provide an excellent environment for three-dimensional cartilage tissue architecture that can promote the chondrogenic differentiation of adult human bone marrow-derived cells. ß

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Comparison of Chondrogensis in Static and Perfused Bioreactor Culture

Cited by 246 publications

References 0 publications

Cardiac tissue engineering using perfusion bioreactor systems

Cardiac tissue engineering using perfusion bioreactor systems

Computational Study of Culture Conditions and Nutrient Supply in Cartilage Tissue Engineering

Rotating three‐dimensional dynamic culture of adult human bone marrow‐derived cells for tissue engineering of hyaline cartilage

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