Comparison of Chondrocytes in Knee Osteoarthritis and Regulation by Scaffold Pore Size and Stiffness

Yu, Zhiwen; You, Zhifeng; Xing, Dan; Li, Jiao Jiao; Zhang, Qingxi; Huang, Hesuyuan; Li, Zhikun; Jiang, Shumeng; Wu, Zhaozhao; Zhang, Yuying; Li, Wenjing; Zhang, Lin; Du, Yanan; Lin, Jianhao

doi:10.1089/ten.tea.2020.0085

Cited by 9 publications

(11 citation statements)

References 40 publications

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“…Our results revealed that collagen biosynthetic process was up‐regulated while oxidative phosphorylation was down‐regulated in the SF‐MMT group compared to the SF group (Figure S5A,B, Supporting Information), which was shown to facilitate the maintenance of chondrocyte phenotype. [ 9c,53b ] Besides, we also observed that some biological processes and pathways related to lipid metabolism were suppressed in the SF‐MMT group compared to the SF group, including fatty acid beta oxidation, fatty acid beta oxidation using acyl CoA dehydrogenase, fatty acid metabolism, and lipid oxidation (Figure S5C, Supporting Information). The increased fatty acid beta oxidation was found in OA chondrocytes with enhanced catabolic processes.…”

Section: Resultsmentioning

confidence: 91%

“…Consistent with our results, previous studies also found that these signaling pathways were associated with the maintenance of the chondrocyte phenotype. [ 9c,53 ] Ling et al. investigated the transcriptome changes in the chondrocytes cultured on different platforms by RNA‐seq and found that the differentiated chondrocytes in monolayer culture had down‐regulated WNT, Notch, and TGF‐ β signaling pathways, indicating their potential roles in maintaining chondrocyte phenotype.…”

Section: Resultsmentioning

confidence: 99%

“…investigated the transcriptome changes in the chondrocytes cultured on different platforms by RNA‐seq and found that the differentiated chondrocytes in monolayer culture had down‐regulated WNT, Notch, and TGF‐ β signaling pathways, indicating their potential roles in maintaining chondrocyte phenotype. [ 53b ] Zhao et al. examined the RNA and protein expression of chondrocytes in lesions and remote areas of OA patients by RNA‐seq and proteomic analysis, and the PI3K‐AKT signaling pathway was enriched by the differentially expressed genes and proteins of chondrocytes in different areas, indicating their possible role in inducing hypertrophic phenotype of chondrocytes in OA.…”

Section: Resultsmentioning

confidence: 99%

“…examined the RNA and protein expression of chondrocytes in lesions and remote areas of OA patients by RNA‐seq and proteomic analysis, and the PI3K‐AKT signaling pathway was enriched by the differentially expressed genes and proteins of chondrocytes in different areas, indicating their possible role in inducing hypertrophic phenotype of chondrocytes in OA. [ 53a ] Besides, previous studies revealed that silicate‐based bioceramics maintained the phenotype of chondrocytes, which could be mediated by TGF‐ β signaling pathway. [ 9c ] Thus, SF‐MMT hydrogels could maintain chondrocyte phenotype by regulating these signaling pathways, including WNT, Notch, TGF‐ β , and PI3K‐AKT signaling pathways.…”

Section: Resultsmentioning

confidence: 99%

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Nanosilicate‐Reinforced Silk Fibroin Hydrogel for Endogenous Regeneration of Both Cartilage and Subchondral Bone

Sheng

Chen

Wang

et al. 2022

Adv Healthcare Materials

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Osteochondral defects are characterized by injuries to both cartilage and subchondral bone, which is a result of trauma, inflammation, or inappropriate loading. Due to the unique biological properties of subchondral bone and cartilage, developing a tissue engineering scaffold that can promote dual-lineage regeneration of cartilage and bone simultaneously remains a great challenge. In this study, a microporous nanosilicate-reinforced enzymatically crosslinked silk fibroin (SF) hydrogel is fabricated by introducing montmorillonite (MMT) nanoparticles via intercalation chemistry. In vitro studies show that SF-MMT nanocomposite hydrogel has improved mechanical properties and hydrophilicity, as well as the bioactivities to promote the osteogenic differentiation of bone marrow mesenchymal stem cells and maintain chondrocyte phenotype compared with SF hydrogel. Global proteomic analysis verifies the dual-lineage bioactivities of SF-MMT nanocomposite hydrogel, which are probably regulated by multiple signaling pathways. Furthermore, it is observed that the biophysical interaction of cells and SF-MMT nanocomposite hydrogel is partially mediated by clathrin-mediated endocytosis and its downstream processes. In vivo, the SF-MMT nanocomposite hydrogel effectively promotes osteochondral regeneration as evidenced by macroscopic, micro-CT, and histological evaluation. In conclusion, a functionalized SF-MMT nanocomposite hydrogel is developed with dual-lineage bioactivity for osteochondral regeneration, indicating its potential in osteochondral tissue engineering.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Nanosilicate‐Reinforced Silk Fibroin Hydrogel for Endogenous Regeneration of Both Cartilage and Subchondral Bone

Sheng

Chen

Wang

et al. 2022

Adv Healthcare Materials

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“…We previously found that five minutes of dipping was adequate for PEGDA penetration in a chip of 5 mm diameter and 2 mm height [ 10 ], and herein we demonstrated the stable form of PEG-infiltrated gels with greater mechanical stiffness constructs. In addition, chondrocyte culture in stiffer matrix material was found to be positively impact to chondrogenesis regarding the nature of articular cartilage as a weight-bearing structure [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

PEG Reinforced Scaffold Promotes Uniform Distribution of Human MSC-Created Cartilage Matrix

Riewruja

Aguglia

Hines

et al. 2022

Gels

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Previously, we used a gelatin/hyaluronic acid (GH)-based scaffold to induce chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSC). The results showed that hBMSCs underwent robust chondrogenesis and facilitated in vivo cartilage regeneration. However, it was noticed that the GH scaffolds display a compressive modulus that is markedly lower than native cartilage. In this study, we aimed to enhance the mechanical strength of GH scaffolds without significantly impairing their chondrosupportive property. Specifically, polyethylene glycol diacrylate (PEGDA) and photoinitiators were infiltrated into pre-formed hBMSC-laden GH scaffolds and then photo-crosslinked. Results showed that infiltration of PEG at the beginning of chondrogenesis significantly increased the deposition of glycosaminoglycans (GAGs) in the central area of the scaffold. To explore the mechanism, we compared the cell migration and proliferation in the margin and central areas of GH and PEG-infiltrated GH scaffolds (GH+PEG). Limited cell migration was noticed in both groups, but more proliferating cells were observed in GH than in GH+PEG. Lastly, the in vitro repairing study with bovine cartilage explants showed that PEG- impregnated scaffolds integrated well with host tissues. These results indicate that PEG-GH hybrid scaffolds, created through infiltrating PEG into pre-formed GH scaffolds, display good integration capacity and represent a new tool for the repair of chondral injury.

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Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts

Ma,

Lin,

Wang

et al. 2024

Cell Tissue Bank

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Osteochondral allograft (OCA) transplantation involves grafting of natural hyaline cartilage and supporting subchondral bone into the cartilage defect area to restore its biomechanical and tissue structure. However, differences in biomechanical properties and donor-host matching may impair the integration of articular cartilage (AC). This study analyzed the biomechanical properties of the AC in different regions of different sites of the knee joint and provided a novel approach to OCA transplantation. Intact stifle joints from skeletally mature pigs were collected from a local abattoir less than 8 h after slaughter. OCAs were collected from different regions of the joints. The patella and the tibial plateau were divided into medial and lateral regions, while the trochlea and femoral condyle were divided into six regions. The OCAs were analyzed and compared for Young’s modulus, the compressive modulus, and cartilage thickness. Young’s modulus, cartilage thickness, and compressive modulus of OCA were significantly different in different regions of the joints. A negative correlation was observed between Young's modulus and the proportion of the subchondral bone (r = − 0.4241, P < 0.0001). Cartilage thickness was positively correlated with Young’s modulus (r = 0.4473, P < 0.0001) and the compressive modulus (r = 0.3678, P < 0.0001). During OCA transplantation, OCAs should be transplanted in the same regions, or at the closest possible regions to maintain consistency of the biomechanical properties and cartilage thickness of the donor and recipient, to ensure smooth integration with the surrounding tissue. A 7 mm depth achieved a higher Young's modulus, and may represent the ideal length.

show abstract

Comparison of Chondrocytes in Knee Osteoarthritis and Regulation by Scaffold Pore Size and Stiffness

Cited by 9 publications

References 40 publications

Nanosilicate‐Reinforced Silk Fibroin Hydrogel for Endogenous Regeneration of Both Cartilage and Subchondral Bone

Nanosilicate‐Reinforced Silk Fibroin Hydrogel for Endogenous Regeneration of Both Cartilage and Subchondral Bone

PEG Reinforced Scaffold Promotes Uniform Distribution of Human MSC-Created Cartilage Matrix

Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts

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