Comparison of chlorogenic acid and rutin contents and antioxidant activity of Dendropanax morbiferus extracts according to ethanol concentration

Abstract

This study investigated the phenolics (TP) and phytochemical contents and radical-scavenging activity of ethanol extracts of Dendropanax morbiferus parts (leaf, stem, and root). The contents of chlorogenic acid (CA) and rutin (RT) and radical-scavenging activity of all extracts by parts were in the order of leaf, stem and root. In the case of leaf, the TP contents were 2.15-2.52 mg/g when extracted with hot water and ethanol concentration (30-70%), and there was no difference depending on extraction solvents and times. However, 95% alcohol extracts showed the lowest content of TP (extraction time: 2-4 h). Particularly, CA and RT contents of 70% ethanolic extract for 2 h were the highest as 12.33 mg/g and 14.01 mg/g, respectively, in which DPPH and ABTS radical scavenging activities were also highest (85% and 110%), respectively.

Antihyperuricemic Effect of Dendropanax morbifera Leaf Extract in Rodent Models

“…Evidence-Based Complementary and Alternative Medicine as high as those of Hwang et al [41] (Table 1). is showed that the difference in CGA content and rutin depends on the harvesting location, season, and extraction conditions of DML [42].…”

“…Rutin is an essential flavonoid in herbal foods, which significantly reduced SUA levels in mice with hyperuricemia [39] and increased urinary UA concentration in rats with renal dysfunction [40]. In a previous study [41], the CGA and rutin contents from DML extracts in 70% ethanol for 2 h were the highest at 12.33 and 14.09 mg/g, respectively. In another study, the CGA concentration in the 30% DML ethanol extract collected in November was the highest at 36.55 mg/g, and the rutin content in the 60% DML ethanol extract collected in May was the highest at 116.71 mg/g [42].…”

Anti-Periodontitis Effects of Dendropanax morbiferus H.Lév Leaf Extract on Ligature-Induced Periodontitis in Rats

“…Extracts were prepared from DMLs as previously described [ 12 ]. Briefly, after washing and drying fresh DMLs, these were extracted using hot-water.…”

“…Total polyphenol content was quantitated, following a previously published protocol [ 12 ]. Briefly, 1 mL of the DML extracts was diluted with 9 mL water and oxidized with 1 mL of diluted Folin-Ciocalteu reagent (Sigma-Aldrich) for 8 min at room temperature followed by neutralization with 4 mL sodium carbonate solution (7.5%, w / v ).…”

“…Nitric oxide (NO) and prostaglandins (PGs) are important mediators of inflammatory responses [ 12 ]. Cytokines, prostaglandin E 2 (PGE 2 ), lysosomal enzymes, and free radicals have been implicated in inflammatory reactions.…”