Comparison of Characteristics of Hexavalent Chromium-tolerant Bacterium,<i>Pseudomonas ambigua</i>G-1, and Its Hexavalent Chromium-sensitive Mutant

Horitsu, Hiroyuki; Futo, Satoshi; Ozawa, Kazuhiro; Kawai, Keiichi

doi:10.1080/00021369.1983.10866051

Search citation statements

Order By: Relevance

Paper Sections

Select...

Enzymatic Reduction Of Hexavalent Chromium By Hexavalent1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1987

Publication Types

Select...

Article1

Relationship

Self Cite1

Independent0

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 ) We reported previously that Cr 6 + toleraht Pseudomonas ambigua G-I had a thick envelope which prevented the permeation of Cr 6 + into the cells. 7 ) In this paper, we describe another mechanism of Cr 6 + tolerance in this bacterum, that is, the direct reduction of Cr 6 + to Cr 3 + by a cell-free extract of P. ambigua G-l.…”

Section: Enzymatic Reduction Of Hexavalent Chromium By Hexavalentmentioning

confidence: 99%

Enzymatic Reduction of Hexavalent Chromium by Hexavalent Chromium TolerantPseudomonas ambiguaG-1

Horitsu

Futo

Miyazawa

et al. 1987

Agricultural and Biological Chemistry

Self Cite

View full text Add to dashboard Cite

When hexavalent chromium (Cr 6 +) tolerant Pseudomonas ambigua G-I was cultivated in nutrient broth containing 150 ppm Cr 6 +, the Cr 6 + content of the broth rapidly decreased. The Cr 6 + reducing enzyme found in a cell-free extract of P. ambigua G-I required NADH but not NADPH as a hydrogen donor for the reduction of Cr 6 +. The specific activities of cell-free extracts of several C~ + sensitive mutants derived from P. ambigua G-l showed decreases to one fourth to one tenth of that of P. ambigua G-1. Glucose protected the Cr 6 + reducing enzyme against inactivation on dialysis.

show abstract