Comparison of Cervical Levels of Interleukins-6 and -8 in Patients with and without Cervical Intraepithelial Neoplasia

Abstract

Introduction: Interleukins-6 and -8 are two pro-inflammatory cytokines increasing in serum and local levels under malignant conditions. There are limited evidences on the association between cervical level of these two factors and cervical intraepithelial neoplasia (CIN). So, this study aimed to explore the association between cervical levels of IL-6 and IL-8 with cervical premalignant lesions. Methods: The present case-control study was conducted on married women undergone Pap smear for routine screening in two groups as the group with CIN (n=100) and the healthy control group (n=100). Cervical secretions were collected using sterile swab and the levels of IL-8 and IL-6 were measured by enzymelinked immunosorbent assay (ELISA). The obtained data were analyzed by SPSS software. Results: The mean cervical IL-6 level was 568.66±594.62 pg/ml in the patients with CIN and 212.7±213.9 pg/ml in the controls (P <0.001). The cervical IL-8 levels in the case and control groups were measured to be 1320.43±876.5 pg/ml and 1053.59±747.64 pg/ ml, respectively (p=0.02). By modifying the confounding size effect of the age and marital duration, it was determined that cervical levels of IL-6 and IL-8 were both associated with CIN. Conclusion: Our results showed that the cervical levels of IL-6 and IL-8 are associated with CIN independent of age and marital duration.

Piperine Reduces Neoplastic Progression in Cervical Cancer Cells by Downregulating the Cyclooxygenase 2 Pathway

“…Other molecular mediators associated with cell proliferation are the cytokines IL-1β and IL-8, which are excessively secreted in cervical cancer and contribute to metastasis, angiogenesis, and cell survival [ 37 , 38 , 39 , 40 , 41 , 42 ]. Furthermore, HPV16 E6 and E7 oncogenes are associated with these mechanisms as they degrade mediators involved in the control of cell proliferation and apoptosis [ 5 ].…”

“…When excessively secreted, the inflammatory cytokines IL-1β, IL-8, and MCP-1 signal the synthesis of metalloproteinases and subsequently increase migration [ 37 , 38 , 39 , 42 , 61 , 62 ] Therefore, while piperine negatively modulated MMPs, it also reduced the secretion of these cytokines, exerting a dual response over metastatic control.…”

Randomized, Double-Blind, Placebo-Controlled Trial of MUC1 Peptide Vaccine for Prevention of Recurrent Colorectal Adenoma

“…They have been shown previously to be elevated in the sera of cancer patients (32, 33) and to modulate response to immunotherapy (34, 35). More recently they have also been found in premalignant disease, such as in individuals with cervical epithelial neoplasms (36), oral premalignant lesions (37, 38), and Barrett’s esophagus (39). The increased levels of these cytokines in non-responders compared to responders suggests that even in the setting of premalignancy, vaccines may need to be combined with other non-toxic approaches to modulating immunosuppression.…”

Piperine Reduces Neoplastic Progression in Cervical Cancer Cells by Downregulating the Cyclooxygenase 2 Pathway

“…Other molecular mediators associated with cell proliferation are the cytokines IL-1β and IL-8, which are excessively secreted in cervical cancer and contribute to metastasis, angiogenesis, and cell survival [37][38][39][40][41][42]. Furthermore, HPV16 E6 and E7 oncogenes are associated with these mechanisms as they degrade mediators involved in the control of cell proliferation and apoptosis [5].…”

“…When excessively secreted, the inflammatory cytokines IL-1β, IL-8, and MCP-1 signal the synthesis of metalloproteinases and subsequently increase migration [37][38][39]42,61,62] Therefore, while piperine negatively modulated MMPs, it also reduced the secretion of these cytokines, exerting a dual response over metastatic control.…”