Comparison of cellular strain with applied substrate strain in vitro

Wall, Michelle E.; Weinhold, Paul S.; Siu, Tung; Brown, Thomas; Banes, Albert J.

doi:10.1016/j.jbiomech.2005.10.032

Cited by 55 publications

(51 citation statements)

References 24 publications

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“…This system can have a better control of waveforms at low and high amplitudes through a computer-regulated bioreactor. Although the Flexcell system does have some limitations (Vande Geest et al, 2004;Wall et al, 2007;Milne et al, 2009;Saminathan et al, 2012), this system still remains the most effective way to screen cells for the expression of mechano-responsive gene in vitro methodology.…”

Section: Discussionmentioning

confidence: 99%

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Han

Sheng

et al. 2013

DNA and Cell Biology

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Physiological occlusal force constitutively exists in the oral environment and is important for periodontal homeostasis and remodeling. Cyclic tensile stress (CTS) triggers the biological response of periodontal ligament (PDL). However, a few reports have studied the correlation between CTS during physiological occlusal force and PDL cell activities such as osteogenic differentiation. In the present study, human PDL cells (hPDLCs) were subjected to 10% elongation CTS loading at 0.5 Hz for 24 h, which represents the physiological conditions of occlusal force. Gene expression microarray was used to investigate the mechano-induced differential gene profile and pathway analysis in vitro. The osteogenic relative factors, that is, SPP1, RUNX2, and SP7, were assessed by real-time PCR and Western blot. The involvement of mitogen-activated protein kinase (MAPK) signaling pathways was investigated by Western blot with a specific inhibitor. The expressions of SPP1, RUNX2, SP7, p-ERK1/2, and p-Elk1 were up-regulated after 10% CTS exposure. However, these up-regulated expressions were prevented by ERK1/2 inhibitor U0126 in the physiological occlusal force-applied hPDLCs. These results showed that 10% CTS could enhance osteogenic differentiation of hPDLCs via ERK1/2-Elk1 MAPK pathway, indicating that CTS during physiological occlusal force is a potent agent for PDL remodeling.

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Section: Discussionmentioning

confidence: 99%

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Han

Sheng

et al. 2013

DNA and Cell Biology

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“…These include the advent of two-dimensional (2D) substrates for cell culture that spans a range of physiologic stiffnesses 3,7 that can be used to apply force to cells, and whose deformations can be used to report cellular forces. [8][9][10][11] However, despite their utility, these tools are not well suited to address the broader field of tissue remodeling and morphogenesis because the complex reorganization and deformations that occur in 3D are not captured in these 2D settings.…”

Section: Introductionmentioning

confidence: 99%

Magnetic approaches to study collective three-dimensional cell mechanics in long-term cultures (invited)

Zhao

Boudou

Wang

et al. 2014

Journal of Applied Physics

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Contractile forces generated by cells and the stiffness of the surrounding extracellular matrix are two central mechanical factors that regulate cell function. To characterize the dynamic evolution of these two mechanical parameters during tissue morphogenesis, we developed a magnetically actuated micro-mechanical testing system in which fibroblast-populated collagen microtissues formed spontaneously in arrays of microwells that each contains a pair of elastomeric microcantilevers. We characterized the magnetic actuation performance of this system and evaluated its capacity to support long-term cell culture. We showed that cells in the microtissues remained viable during prolonged culture periods of up to 15 days, and that the mechanical properties of the microtissues reached and maintained at a stable state after a fast initial increase stage. Together, these findings demonstrate the utility of this microfabricated bio-magneto-mechanical system in extended mechanobiological studies in a physiologically relevant 3D environment. V C 2014 AIP Publishing LLC.

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“…Given the small footprint beneath the cell, tensile stretching of individual adherent cells is technically demanding, and schemes that allow larger cell substrates to be actuated while retaining the cell within a microscopic field of view have begun to emerge (Serrell et al 2007;Wall et al 2007;Gerstmair et al 2009). However, until now these approaches have involved the application of nonhomogenous substrate strains, and more importantly have failed to yield viable measures of cell stiffness.…”

Section: Introductionmentioning

confidence: 99%

A novel method for assessing adherent single-cell stiffness in tension: design and testing of a substrate-based live cell functional imaging device

Bartalena

Grieder

Sharma

et al. 2010

Biomed Microdevices

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Various micro-devices have been used to assess single cell mechanical properties. Here, we designed and implemented a novel, mechanically actuated, two dimensional cell culture system that enables a measure of cell stiffness based on quantitative functional imaging of cellsubstrate interaction. Based on parametric finite element design analysis, we fabricated a soft (5 kPa) polydimethylsiloxane (PDMS) cell substrate coated with collagen-I and fluorescent micro-beads, thus providing a favorable terrain for cell adhesion and for substrate deformation quantification, respectively. We employed a real-time tracking system that analyzes high magnification images of living cells under stretch, and compensates for gross substrate motions by dynamic adjustment of the microscope stage. Digital image correlation (DIC) was used to quantify substrate deformation beneath and surrounding the cell, leading to an estimate of cell stiffness based upon the ability of the cell to resist the applied substrate deformation. Sensitivity of the system was tested using chemical treatments to both "soften" and "stiffen" the cell cytoskeleton with either 0.5 μg/ml Cytochalasin-D or 3% Glutaraldehyde, respectively. Results indicate that untreated osteosarcoma cells (SAOS-2) exhibit a 1.5±0.7% difference in strain from an applied target substrate strain of 8%. Compared to untreated cells, those treated with Cyochalasin-D passively followed the substrate (0.5±0.5%, p<0.001), whereas Glutaraldehyde enhanced cellular stiffness and the ability to resist the substrate deformation (2.9 ± 1.6%, p < 0.001). Nanoindentation testing showed differences in cell stiffness based on culture treatment, consistent with DIC findings. Our results indicate that mechanics and image analysis approaches do hold promise as a method to quantitatively assess tensile cell constitutive properties.

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Comparison of cellular strain with applied substrate strain in vitro

Cited by 55 publications

References 24 publications

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Magnetic approaches to study collective three-dimensional cell mechanics in long-term cultures (invited)

A novel method for assessing adherent single-cell stiffness in tension: design and testing of a substrate-based live cell functional imaging device

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