Comparison of cellular mechanisms induced by pharmaceutical exposure to caffeine and its combination with salicylic acid in mussel Mytilus galloprovincialis

Afsa, Sabrine; Galati, Mariachiara; Billè, Barbara; Parrino, Vincenzo; Mansour, Hédi Ben; Cappello, Tiziana

doi:10.1016/j.etap.2022.103888

Cited by 13 publications

(3 citation statements)

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“…Being stained by Masson trichrome with aniline blue, the chitinous rod is made up of connective tissue, and its break could be related to mechanical damage of MPs since it has been demonstrated that they can reach this anatomical district (Von Moos et al, 2012). The hemocyte infiltration could be caused not only by the break of the chitinous rod but also as a response of defense from pollutants, indeed it is caused also by caffeine as demonstrated by our results and other experimentation (De Marco, Afsa, Galati, Billè, & Parrino, 2022; De Marco, Afsa, Galati, Guerriero, et al, 2022) and it is also provoked by other pollutants (De Marco et al, 2023; Speciale et al, 2018). Another defense mechanism could be the hemocytic aggregation in the gill filament after caffeine exposure which impairs hemolymph flow inside the filament.…”

Section: Discussionsupporting

confidence: 80%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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Section: Discussionsupporting

confidence: 80%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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“…The supernatants were collected and kept at −20 °C until the analysis of oxidative stress biomarkers [ 52 ] including lipid peroxidation (LPO) [ 56 ], glutathione (GSH) [ 57 ], and catalase (CAT) [ 58 ] by applying their respective protocols. Specifically, to estimate LPO, a measurement of malondialdehyde (MDA; nmol/mg protein) was performed by applying the thiobarbituric acid reactive (TBARS) method as described in detail elsewhere [ 59 , 60 ]. The reduced GSH was estimated using the colorimetric Ellman’s method [ 61 ], treating the tissue homogenate with trichloroacetic acid (TCA) 10%, and then adding 0.2 M Tris EDTA buffer in order to prevent GSH oxidation.…”

Section: Methodsmentioning

confidence: 99%

“…The reduced GSH was estimated using the colorimetric Ellman’s method [ 61 ], treating the tissue homogenate with trichloroacetic acid (TCA) 10%, and then adding 0.2 M Tris EDTA buffer in order to prevent GSH oxidation. In addition, the enzymatic activity of CAT (μmol/min/mg protein) was evaluated following the dismutation of H 2 O 2 at 240 nm for 90 s using a colorimetric technique [ 59 , 60 ].…”

Section: Methodsmentioning

confidence: 99%

Ameliorative Hematological and Histomorphological Effects of Dietary Trigonella foenum-graecum Seeds in Common Carp (Cyprinus carpio) Exposed to Copper Oxide Nanoparticles

Noureen

Rehman

Jabeen

et al. 2022

IJERPH

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Different types of metal oxide nanoparticles (NPs) are being used for wastewater treatment worldwide but concerns have been raised regarding their potential toxicities, especially toward non-targeted aquatic organisms including fishes. Therefore, the present study aimed to evaluate the toxicity of copper oxide (CuO) NPs (1.5 mg/L; positive control group) in a total of 130 common carp (Cyprinus carpio), as well as the potential ameliorative effects of fenugreek (Trigonella foenum-graecum) seed extracts (100 mg/L as G-1 group, 125 mg/L as G-2 group, and 150 mg/L as G-3 group) administered to fish for 28 days. Significant changes were observed in the morphometric parameters: the body weight and length of the CuO-NP-treated fish respectively decreased from 45.28 ± 0.34 g and 14.40 ± 0.56 cm at day one to 43.75 ± 0.41 g and 13.57 ± 0.67 cm at day 28. Conversely, fish treated with T. foenum-graecum seed extract showed significant improvements in body weight and length. After exposure to CuO NPs, a significant accumulation of Cu was recorded in the gills, livers, and kidneys (1.18 ± 0.006 µg/kg ww, 1.38 ± 0.006 µg/kg ww, and 0.05 ± 0.006 µg/kg ww, respectively) of the exposed common carp, and significant alterations in fish hematological parameters and oxidative stress biomarkers (lipid peroxidation (LPO), glutathione (GSH), and catalase (CAT)) were also observed. However, supplementing diets with fenugreek extracts modulated the blood parameters and the oxidative stress enzymes. Similarly, histological observations revealed that sub-lethal exposure to CuO NPs caused severe histomorphological changes in fish gills (i.e., degenerative epithelium, fused lamellae, necrotic lamellae, necrosis of primary lamellae, complete degeneration, and complete lamellar fusion), liver (i.e., degenerative hepatocytes, vacuolization, damaged central vein, dilated sinusoid, vacuolated degeneration, and complete degeneration), and kidney (i.e., necrosis and tubular degeneration, abnormal glomerulus, swollen tubules, and complete degeneration), while the treatment with the fenugreek extract significantly decreased tissue damage in a dose-dependent manner by lowering the accumulation of Cu in the selected fish tissues. Overall, this work demonstrated the ameliorative effects of dietary supplementation with T. foenum-graecum seed extract against the toxicity of NPs in aquatic organisms. The findings of this study therefore provided evidence of the promising nutraceutical value of fenugreek and enhanced its applicative potential in the sector of fish aquaculture, as it was shown to improve the growth performance and wellness of organisms.

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Time- and dose-dependent biological effects of a sub-chronic exposure to realistic doses of salicylic acid in the gills of mussel Mytilus galloprovincialis

Afsa

Galati

Guerriero

et al. 2022

Environ Sci Pollut Res

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Comparison of cellular mechanisms induced by pharmaceutical exposure to caffeine and its combination with salicylic acid in mussel Mytilus galloprovincialis

Cited by 13 publications

References 100 publications

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Ameliorative Hematological and Histomorphological Effects of Dietary Trigonella foenum-graecum Seeds in Common Carp (Cyprinus carpio) Exposed to Copper Oxide Nanoparticles

Time- and dose-dependent biological effects of a sub-chronic exposure to realistic doses of salicylic acid in the gills of mussel Mytilus galloprovincialis

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